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1
Content available Characteristic of fish collagen films cross-linked with glutaraldehyde
Kulka-Kamińska Karolina, Brudzyńska Patrycja, Sionkowska Alina, Lewandowska Katarzyna, Piwowarski Łukasz
Engineering of Biomaterials
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2023
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vol. 26, no. 168
25--32
EN
Collagen is a valuable biopolymer in many fields, especially in biomedical sciences. Thanks to its biodegradability and high biocompatibility, it is a desirable material for applications that require contact with the human body. There are many sources of collagen, of which marine-origin collagen has become an important one in recent times. Pure collagen has poor stability and is sensitive to the effects of heat and other external factors. The cross-linking process can improve the properties of collagen materials. Many different methods of cross-linking can be distinguished, including chemical ones. In this study, we were concerned to obtain collagen films modified with glutaraldehyde (GTA). The influence of this additive on the chemical, mechanical, swelling, and hydrophilic properties of the biopolymeric matrix was evaluated. Two different concentrations of collagen were used, as well as three different concentrations of GTA. Results of the analysis showed that the properties of the obtained films were affected by the addition of even a small amount of cross-linker. Spectroscopic measurements indicated minor changes that reflect interactions between GTA and the collagen matrix. Mechanical tests showed changes for modified samples in values of tensile strength, breaking force, and elongation at break. The hydrophilicity decreased slightly for films with GTA. The durability of the modified samples in the swelling test increased. Differences between 1% and 2% collagen films with additives were also observed. The GTA-obtained fish collagen films can be promising materials for biomedical applications.
2
Content available Mechanical properties of the porcine pericardium extracellular matrix cross-linked with glutaraldehyde and tannic acid
Dąbrowska Karolina Zofia, Turek Artur, Grzejda Rafał, Kobielarz Magdalena
Acta of Bioengineering and Biomechanics
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2022
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Vol. 24, no. 3
21--31
EN
The aim of this study was to determine the influence of factors such as temperature and glutaraldehyde (GA) concentration on the mechanical properties of porcine pericardia, in order to propose the recommended optimal conditions of a cross-linking process. It was also to verify whether tannic acid (TA), a natural cross-linking agent that stabilizes collagenous tissues by a different mechanism than GA, may positively influence the strength of pericardium. Methods: The samples were incubated at various temperatures (4, 22, and 37 °C) and GA concentration solutions (0.6, 1.5 and 3%) for 7 days. Three series were selected and additionally cross-linked with 0.3% TA for another 7 days. Mechanical properties of cross-linked pericardium samples, i.e., ultimate tensile strength (UTS) and elastic modulus (E) were measured in uniaxial tensile testing. The hyperelastic model for incompressible materials – isotropic by Ogden [24] and anisotropic by Fung [7] were utilized to describe the mechanical behaviour of treated pericardium. Results: The temperature has an influence on cross-linking effects; the lowest values of UTS were reported for specimens cross-linked at 22 °C, while the mechanical properties of series treated at 4 °C or 37 °C were comparable. At a particular temperature of incubation, the GA concentrations have not affected the mechanical properties of tissues. The dependence between mechanical parameters and agent concentration was only observed for specimens treated with GA at 37 °C. Conclusions: The conditions of the cross-linking process affect the mechanical properties of the porcine pericardium. Room temperature (22 °C) and the concentration of 1.5% GA occurred to be ineffective. The mechanical properties of GA-treated pericardium were improved by an additional TA cross-linking.
3
Content available Morphological studies of tissues stabilized by glutaraldehyde and tannic acid
Turek B., Marcinkowski A., Cwalina B., Nożyński J., Dzierżewicz Z.
Engineering of Biomaterials
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2009
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Vol. 12, no. 89-91
32--34
EN
Despite the disadvantages of glutaraldehyde (GA)-stabilization of tissues, it is the method most often used for xenogeneic tissues preparation. Nowadays, partial elimination of drawbacks of this method is achieved by using GA in the mixture with other crosslinking reagents, which completes the stabilization effects and acts synergistically. The aim of this work was to determine microstructure and nanostructure of porcine pericardium stabilized by GA and tannic acid (TA). The microstructure was examined by optical microscopy and the nanostructure by atomic force microscopy (AFM). Different results on the level of micro- and nanostructure were observed. No essential changes in the tissue morphology after crosslinking with GA and TA were observed under optical microscope, but significant morphological differences were revealed in AFM studies.
4
Content available Wpływ jonów glinu na strukturę tkanki osierdzia świni, badanej z użyciem EPR - metody znaczników spinowych
Cwalina B., Dul L., Jastrzębska M.
Inżynieria Biomateriałów
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2005
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R. 8, nr 47-53
30--33
PL
Celem pracy była ocena zmian struktury tkanek osierdzia świni, poddanych działaniu jonów glinu (w postaci siarczanu AI2(SO4)3 oraz wodorotlenku AI2O3xnH2O w preparacie ALUSAL), z zastosowaniem spektroskopii EPR - metody znaczników spinowych (znacznik spinowy: 4-izotiocyjaniano-TEMPO; 4ITCTO). Wykazano, że traktowanie tkanki osierdzia świni jonami glinu prowadzi do zwiększenia sztywności struktury wewnętrznej tkanki. Ujawniły to zmiany kształtu widma EPR znacznika 4ITCTO związanego z tkanką. Jakkolwiek efekty stabilizacji tkanki jonami AI3+ i aldehydem glutarowym są podobne, jednak mechanizmy sieciowania białek tkankowych są różne.
EN
The aim of this work was to elucidate changes in the structure of porcine pericardium tissues treated with the aluminium ions (in the form of sulphate salt AI2(SO4)3 or hydroxide AI2O3xnH2O in the ALUSAL preparation), using the EPR spectroscopy - spin labeling method (spin tabel: 4-lsothiocyanato-TEMPO; 4ITCTO). It has been demonstrated that the treatment of the porcine pericardium tissue with aluminium ions leads to enlargement of stiffness of the tissue intemal structure. It was visible by the shape changes in the EPR spectra of 4ITCTO label attached with the tissue. Although results of the tissue stabilization by Al3+ ions and glutaraldehyde are similar, however mechanisms of the tissue proteins cross-linking are different.
5
Content available Stabilność biochemiczna tkanek osierdzia modyfikowanych aldehydem glutarowym lub mrówkowym
Cwalina B., Turek A., Miśkowiec M., Nawrat Z., Domal-Kwiatkowska D.
Inżynieria Biomateriałów
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2002
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R. 5, nr 23-25
64-67
PL
Porównywano stabilność biochemiczną tkanek osierdzia świni: natywnej i modyfikowanych za pomocą aldehydu glutarowego (GA) lub mrówkowego (FA). Analizowano profile elektroforetyczne białek uwalnianych z badanych tkanek, zarówno trawionych i nie poddawanych działaniu pankreatyny. Tkanki modyfikowane były bardziej odporne na trawienie enzymatyczne, niż tkanka natywna. Mała liczba prążków reprezentujących białka niskocząsteczkowe w ekstraktach uzyskanych z tkanek modyfikowanych świadczy o większej stabilności tych tkanek spowodowanej ich silniejszym usieciowaniem. Wydłużenie czasu modyfikacji powodowało zwiększenie efektu stabilizacji tkanek.
EN
Biochemical stability of pericardial tissues native and modified using glutaraldehyde (GA) and formaldehyde (FA) have been compared. The electrophoretic profiles of the proteins released from investigated tissues, both digested and untreated with pancreatin, have been studied. The modified tissues were more resistant to enzymatic digestion as compared with native one. A small number of lines representing low-molecular proteins in extracts obtained from the modified tissues point to these tissues higher stability due to their stronger crosslinking. Prolongation of modification time caused increase in the tissues stabilization effect.
6
Content available Efekty sieciowania osierdzia świni badane za pomocą spektroskopii EPR - metody znakowania spinowego
Cwalina B., Dul L.
Inżynieria Biomateriałów
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2002
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R. 5, nr 23-25
61-64
PL
Celem pracy była ocena udziału eta-aminowych grup lizyny w elastynie, jak również eta-aminowych grup lizyny i hydroksylizyny w kolagenie w modyfikacji tkanki osierdziowej za pomocą glutaraldehydu (GA). Materiałami badanymi były: nierozpuszczalny kolagen typu I, elastyna i osierdzie włókniste świni, zarówno natywne i usieciowane glutaraldehydem. Do badania efektu sieciowania wykorzystano spektroskopię elektronowego rezonansu paramagnetycznego (EPR) - metodę znakowania spinowego (znacznik spinowy: izotiocyjaniano-Tempo; ITCTO). Można stwierdzić, że w procesie sieciowania tkanki kolagenowej przez GA uczestniczą grupy eta-aminowe zarówno lizyny i hydroksylizyny. W czystym kolagenie GA reaguje zarówno z grupami eta-aminowymi hydroksylizyny.
EN
The aim of his work was to elucidate contribution of the etha-amino groups of lysine in elastin as well as of the etha-amino groups of lysine and hydroxylysine in collagen to the pericardial tissue modification by means of glutaraldehyde (GA). The investigated materials were: insoluble collagen type I, elastin and porcine fibrous pericardium, both native and crosslinked with glutaraldehyde. The electron paramagnetic resonance (EPR) spectroscopy - method of spin labeling (spin label: isothiocyanato-Tempo; ITCTO) - has been used for study of the crosslinking-effects. It may be stated that in process of collagenous tissue crosslinking by GA participate etha-amino groups of both lysine and hydroxylysine. In pure collagen, GA reacts mainly with etha-amino groups of hydroxylysine.
7
Content available Wpływ stabilizacji tkanki na profile elektroforetyczne białek ekstrahowanych z osiedzia świni
Cwalina B., Turek A., Sliupkas-Dyrda E., Nawrat Z.
Inżynieria Biomateriałów
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2001
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R. 4, nr 17-19
89-91
PL
Badano profile elektroferyczne białek uwalnianych z tkanek - zarówno natywnych, jak i modyfikowanych, trawionych i nie poddawanych działaniu pankreatyny. Utrwalanie tkanki osierdziowej zarówno dimetylo-imidosuberynianem (DMS), jak i aldehydem glutarowym (GA) skutkowało zwiększeniem masy cząsteczkowej białek ekstrahowanych z modyfikowanych tkanek, w porównaniu z tkankami natywnymi (kontrolnymi). Wiele niskocząsteczkowych białek nie występowało w ekstraktach uzyskanych z tkanek najbardziej utrwalonych. Modyfikowane tkanki były bardziej odporne na trawienie enzymatyczne.
EN
The electrophoretic profiles of the proteins released from tissues - both native and modifies, digested and untreated with pancreatin, have been studied. Fixation of pericardial tissue with dimethyl suberimidate (DMS) as well as with glutaraldehyde (GA) resulted in a higher molecular weight of proteins extracted from the modified tissues as compared with the native (control) tissues. Many low-molecular proteins were not present in extracts obtained from the most fixed tissues. The modified tissues were more resistant to enzymatic digestion.
8
Content available remote Szybki test do oznaczania aktywności aldehydu glutarowego w roztworach dezynfekcyjnych
Łukowska E., Chwojnowski A.
Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
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2000
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Vol. 6, nr 3-4
67-71
PL
Przedstawiono szybko działający i prosty w użyciu test do oznaczania aldehydu glutarowego w roztworach dezynfekcyjnych. Opracowany test wykorzystuje specjalnie zaadaptowaną reakcję aldehydów z chlorowodorkiem hydroksyloaminy. Umożliwia on odróżnienie stężenia aldehydu glutarowego w płynie aktywnym od jego stężenia w płynie wyczerpanym. Wyniki odczytuje się wizualnie przez porównanie barwy testu ze skalą barw. Zapropono-. wano metodę kontroli jakości testu, zweryfikowaną m.in. przez badania przeprowadzone w niezależnym ośrodku. Wnioski wskazują na pełną przydatność opracowanego suchego testu w przewidywanych zastosowaniach.
EN
A quick and easy dry test for glutaraldehyde determination in disinfection liquids has been presented. Reaction between aldehydes and hydroxylamine hydrochloride has been adapted for test. The test enables distinguish between glutaraldehyde concentrations in the active and inactive disinfection liquids. The reading out of the test is done visually by comparison of the test colour to the colour scale. A method of test quality control has been proposed and verified. In conclusion, it was stated that the test can be used for the control of the disinfection liquid based on glutaradehyde.
9
Content available remote Glutaraldehyd
Rolecki R.
Podstawy i Metody Oceny Środowiska Pracy
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1999
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Nr 21
61--100
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