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1 2006

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Carbon-source-dependent synthesis and composition of biosurfactant synthesized by Pseudozyma antarctica

Bednarski W., Narwojsz M., Adamczak M., Nawotka R.

Environmental Biotechnology

2006

Vol. 2, No. 1

31-36

Pseudozyma antarctica (formerly Candida antarctica) ATCC 28323 was cultivated in preselected conditions in a medium containing glucose and products of enzymatically hydrolyzed lactose in milk permeate (after ultrafiltration) supplemented with the waste fats (fish, pork and post-refining fatty acids) to induce and intensify biosurfactant synthesis. The yield (12.0-26.7*g/dm3) of biosurfactant synthesis and the fatty acid composition of its hydrophobic fractions depended on carbon sources, i.e. waste fats listed above which have been added to the medium. The obtained results indicate that the fatty acids from the waste fats used seemed to be directly incorporated in the mannosylerithritol lipids. The main fatty acids present in biosurfactant were C16:0, C18:0, C18:1,C18:2.