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Analysis of Polymorphic DNA Sequences in the Identfication of Individuals and its Possible Use in Biometric Systems

Kartasińska E., Brągoszewska A.

Journal of Telecommunications and Information Technology

2015

nr 2

11--17

The article presents the achievements in DNA forensic science practice, the latest developments as well as future trends. The article concerns also other than forensic DNA applications as well as expectations, concerns and obstacles. DNA identification technology unlike other biometric techniques requires the collection of biological material and the identification is not performed in real time. DNA utilized in most of forensic identification, is present almost in every living cell in the body. What is more, each cell of the same body has the same DNA molecule which means that it is possible to compare the DNA sampled from different sources, for example saliva with blood or semen from the same person. Rapid development and reliability of DNA technology contribute to the fact that the analysis of polymorphic DNA sequences constitutes a very important evidence used in the court. The unique properties of DNA and rapid development of DNA analytical devices allow to claim that DNA may assume a more important position amongst biometric data in the future.

A novel polymorphic mtDNA marker for population studies of the pink shrimp, Farfantepenaeus duorarum (Crustacea, Penaeidae)

Grabowski M., Grater W. D., Stuck K. C.

Oceanologia

2004

No. 46 (1)

147-151

The pink shrimp, Farfantepenaeus duorarum, is one of the most important shrimp species commercially harvested along the Gulf and Atlantic coasts of the US. In this study we developed a mitochondrial marker suitable for population studies of the species. A 611-617 bp hypervariable portion of the AT-rich region of the mt genome was amplified and sequenced. The 617 bp long consensus sequence contained 15 polymorphic insertion/deletion sites and 165 polymorphic substitution sites. Kimura 2-parameter distances ranged from 0.00 to 0.06 with a mean of 0.02. Among the 104 sequences, 100 haplotypes were counted if all mutations were included. If transitions were omitted, 34 haplotypes were observed. The results indicate that the hypervariable portion of the AT-rich region may be an effective marker for revealing the genetic structure of the pink shrimp off the southeast US.