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Manufacturing and characterization of gellan gum – zinc oxide composites as potential biomaterials for wound treatment

Macyk Alicja, Kusibab Anna, Pamuła Elżbieta

Engineering of Biomaterials

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2023

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vol. 26, no. 168

2--8

EN

This study aimed to produce gellan gum-based hydrogels with the addition of zinc oxide as a potential dressing material. Hydrogels with ZnO concentrations of 0.01%, 0.02% and 0.04% were prepared, micrometric and nanometric ZnO particles were used, and a CaCl2 crosslinker was added to one part of the samples. All samples (14 types) produced by the freeze drying method were characterized with high swelling properties (>2000%), what is important to ensure the absorption of exudates from wounds. Samples with ZnO particles cross-linked with CaCl2 lost less mass after incubation in aqueous media and were characterized by better dimensional stability than those without crosslinking. The pH of the extracts of the samples containing ZnO particles was more neutral (pH 7.0-7.6) than that of the control gellan gum samples (pH of 5.5-6.1). The zinc release from cross-linked samples was twice as high for those containing nanometric particles than for micrometric particles (1.94 ± 0.04 mg/l and 0.93 ± 0.02, respectively). Relatively large amounts of released zinc species in the case of samples containing ZnO nanoparticles are promising in the context of the antibacterial properties and treatment of infected wounds. A lower amount of zinc released from samples with ZnO microparticles could be sufficient to prevent the development of the infection. Furthermore, both materials show satisfactory cytocompatibility with L929 fibroblasts, as shown by Alamar blue and live/dead viability tests, making them prospective candidates for wound healing

2

Composite scaffolds enriched with calcium carbonate microparticles loaded with epigallocatechin gallate for bone tissue regeneration

Pietryga Krzysztof, Panaite Anca-Alexandra, Pamuła Elżbieta

Engineering of Biomaterials

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2022

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vol. 25, no. 166

12--21

EN

There is a need to develop advanced multifunctional scaffolds for the treatment of bone tissue lesions, which apart from providing support for infiltrating cells could assure the delivery of drugs or biologically active molecules enhancing bone formation. We developed composite scaffolds for bone tissue engineering based on gellan gum (GG) and gelatin (Gel) hydrogel enriched with epigallocatechin gallate (EGCG) loaded CaCO3 microparticles and subjected to enzymatic mineralization with calcium phosphate (CaP). The method of manufacturing CaCO3 microparticles was optimized. The EGCG-loaded microparticles were smaller than those unloaded, and the release of EGCG was prolonged for up to 14 days, as shown by the Folin-Ciocalteu test. The particles reduced the viability of the MG-63 cells as compared to the control. However, when they were loaded with EGCG, their cytotoxicity was reduced. The particles were suspended in a GG/Gel hydrogel containing alkaline phosphatase (ALP), soaked in calcium glycerophosphate (CaGP) solution to create CaP deposits, and submitted to freeze-drying, in order to produce a porous scaffold. The microstructure of the scaffolds was characterized by optical and scanning electron microscopy and showed that the size of the pores corresponds to that of the spongy bone. In vitro tests with MG-63 cells confirmed that mineralized scaffolds support cell adhesion and growth to a higher extent than nonmineralized ones.

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Phenolic plant extract enrichment of enzymatically mineralized hydrogels

Douglas Timothy E.L, Lopez-Heredia Marco A., Pułczyńska Aleksandra, Łapa Agata, Pietryga Krzysztof, Schaubroeck David, Santos Sónia A.O., Pais Adriana, Brackman Gilles, Schamphelaere Karel de, Samal Sangram Keshari, Keppler Julia K., Bauer Jonas L, Chai Feng, Blanchemain Nicolas, Coenye Tom, Pamuła Elżbieta, Skirtach Andre G

Engineering of Biomaterials

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2019

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Vol. 22, no. 149

2--9

EN

Hydrogel mineralization with calcium phosphate (CaP) and antibacterial activity are desirable for applications in bone regeneration. Mineralization with CaP can be induced using the enzyme alkaline phosphatase (ALP), responsible for CaP formation in bone tissue. Incorporation of polyphenols, plant-derived bactericidal molecules, was hypothesized to provide antibacterial activity and enhance ALP-induced mineralization. Three phenolic rich plant extracts from: (i) green tea, rich in epigallocatechin gallate (EGCG) (herafter referred to as EGCG-rich extract); (ii) pine bark and (iii) rosemary were added to gellan gum (GG) hydrogels and subsequently mineralized using ALP. The phenolic composition of the three extracts used were analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MSn). EGCG-rich extract showed the highest content of phenolic compounds and promoted the highest CaP formation as corroborated by dry mass percentage meassurements and ICP-OES de-termination of mass of elemental Ca and P. All three extracts alone exhibited antibacterial activity in the following order EGCG-rich > PI > RO, respectively. However, extract-loaded and mineralized GG hydro-gels did not exhibit appreciable antibacterial activity by diffusion test. In conclusion, only the EGCG-rich extract promotes ALP-mediated mineralization.

4

Wykorzystanie biodegradowalnych polimerów w rozmnażaniu ozdobnych roślin cebulowych

Salachna P.

Inżynieria Ekologiczna

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2016

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Nr 46

143--148

PL

Syntetyczne regulatory wzrostu mają negatywny wpływ na środowisko stąd coraz częściej w ogrodnictwie wykorzystuje się naturalne biostymulatory. Niektóre naturalne polimery wykazują stymulujący wpływ na wzrost i rozwój roślin. Związki te mogą być stosowane do tworzenia hydrożelowych otoczek na powierzchni organów roślinnych w celu ochrony przed niekorzystnym wpływem czynników zewnętrznych. Gatunki eukomis są powszechnie stosowane w tradycyjnej medycynie Afryki Południowej i znajdują szerokie zastosowanie jako ozdobne rośliny cebulowe. Celem badań było określenie wpływu otoczkowania w biopolimerach sadzonek dwułuskowych dwóch odmian eukomis czubatej (‘Sparkling Burgundy’ i ‘Twinkly Stars’) na plon i jakość uzyskanych cebul przybyszowych. Sadzonki otoczkowano w 1% roztworze gumy gellanowej (Phytagel) lub 0,5% roztworze oligochitozanu. Stwierdzono, że otoczkowanie sadzonek w gumie gellanowej miało stymulujący wpływ na liczbę i masę cebul przybyszowych. Najsilniejszy system korzeniowy wytworzyły cebule uformowane na sadzonkach otoczkowanych w oligochitozanie. Porównując odmiany wykazano, że ‘Sparkling Burgundy’ wytworzyła więcej cebul, które jednocześnie miały większą masę i dłuższe korzenie niż ‘Twinkly Stars’.

EN

Synthesized growth regulators may cause a negative impact on the environment so the use of natural bio-stimulators in horticulture is becoming more popular. Some biopolymers can have a stimulating influence on the growth and development of plants. In addition, polymers can be used to form hydrogel coatings on the surface of plant organs to protect them against the adverse influence of external factors. Eucomis species are widely used in southern African traditional medicine and have a horticultural potential. This study was conducted in order to determine the effect of biopolymer coating of twin-scale cuttings on the yield of Eucomis comosa ‘Sparkling Burgundy’ and ‘Twinkly Stars’ bulblets. For coating of twin-scale cuttings, 1% solution of gellan gum (Phytagel) or 0.5% solution of oligochitosan were used. Coating of twin-scale cuttings in gellan gum had a positive impact on the number and weight of the bulblets. The highest number of roots and maximum root length were observed in bulblets derived from the twin scale cuttings coating in oligochitosan. A comparison of cultivars showed that ‘Sparkling Burgundy’ produced more bulblets with longer roots than ‘Twinkly Stars’.

5

Wpływ gumy gellan na właściwości reologiczne roztworów skrobi o różnym pochodzeniu botanicznym

Orczykowska M., Budzyński P., Dziubiński M.

Inżynieria i Aparatura Chemiczna

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2011

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Nr 1

31-32

PL

Przedstawiono wyniki badań dotyczących wzajemnych interakcji wodnych roztworów skrobi o różnym pochodzeniu botanicznym z hydrokoloidem po-lisacharydowym, jakim jest jeden z dodatków do żywności - guma gellano-wa. W prezentowanej pracy przeanalizowano uzyskane w trakcie pomiarów reometrycznych właściwości reologiczne badanych roztworów, przedyskutowano efekt synergistyczny, a także oszacowano wartość stałej czasowej X odpowiadającej za podatność struktury na zniszczenie ścinaniem.

EN

Investigation results dealing with the structure of aqueous solution of starch from different botanical sources with presence or without gellan gum are presented in the paper. This gum is generally applied as food additive. The authors present an analysis of rheological properties of starch - hydrocol-loid blends determined during rheometric measurements. The synergistic effect and a value of time constant responsible for the structure of blends are described.

6

Ocena stabilności zawiesiny tiksotropowej w obecności środka zagęszczającego

Orczykowska M., Budzyński P., Dziubiński M.

Inżynieria i Aparatura Chemiczna

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2010

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Nr 2

93-94

PL

Przedstawiono wyniki badań dotyczących stabilności wodnych roztworów skrobi ziemniaczanej w obecności lub nie hydrokoloidu polisacharydowe-go, jakim jest jeden z dodatków do żywności - guma gellanowa. W prezentowanej pracy przeanalizowano uzyskane w trakcie pomiarów reometrycz-nych właściwości reologiczne badanych roztworów, oszacowano wartość statycznej i dynamicznej granicy płynięcia oraz sprężystość utworzonej struktury. Przedyskutowano własności tiksotropowe roztworów poprzez i określenie wielkości pola powierzchni pętli histerezy.

EN

Results of investigations on the effect of stability of aqueous solution of potato starch with a presence or without gellan gum are presented in the paper. This gum is generally applied as food additives. An analysis of rheological properties of starch - hydrocolloid blends determined during rheo-metric measurements is shown. The statical and dynamic yield stress and elasticity of structure of solutions' blends are described. The mechanism of thixotropic phenomenon and thixotropic hysteresis loop (i.e. area of the loop) is discussed.