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An integrated gas removing system for microfluidic application. Design and evaluation

Hofman I. M., Ziółkowska K., Dybko A., Brzózka Z.

Challenges of Modern Technology

2013

Vol. 4, no. 3

10--15

Microfluidic systems are used in a wide range of applications, including medical diagnostics, cell engineering and bioanalytics. In this work we focused on “Lab-on-a-chip” microsystems for cell cultivation. A troublesome problem of gas bubbles entering microdevices causing signal interferences and cells damage was emphasized. A novel, integrated debubbler in the form of cylindrical traps covered with thin PDMS membrane was designed and manufactured. Demonstrated debubbler was successfully applied in a long-lasting culture of HT-29 cell aggregates.