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FISH method for identification of microbes in wastewater distribution systems

Domańska M., Kuhn R., Łomotowski J., Stańczyk E.

Environment Protection Engineering

2014

Vol. 40, nr 3

151--160

The fluorescence in situ hybridization (FISH) method is widely used to identify various types of cells. In comparison to cultivation dependent methods, identification of microbes by FISH is easier and generally takes several hours. A review of improvements to the FISH method has been presented, its advantages and disadvantages, as well as examples of application. Particular consideration was given to the efficiency of identification of microbes in samples taken from sewerage. The effectiveness of the method was confirmed by the results obtained in samples from the membrane bioreactor.

A brief overview of the applicability of FISH methods in environmental engineering

Piórczyk J., Jasionowski B., Stańczyk E.

Structure and Environment

2013

Vol. 5, no. 1

42--45

Fluorescent in situ hybridization (FISH) is a commonly used method for the detection of microorganisms in heterogeneous environment. It is primarily used for quantitative analysis of microorganisms. FISH gives possibilities of evaluating activities of some bacteria and their physiological state in the environment. In the recent years, ftuorescent in situ hybridization has been improved and adapted for the research on environmental samples.