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Rapid capture and exemplary detection of clinical pathogen using surface modified fluorescent silica coated iron oxide nanoparticles

Chitra K., Annadurai G.

Biocybernetics and Biomedical Engineering

2014

Vol. 34, no. 4

230--237

Rapid, sensitive and selective detection of pathogenic bacteria is very important for treat- ment of diseases like foodborne illness, sepsis and bioterrorism. The silica coated iron oxide nanoparticles (SIO) were synthesized using simple, cost-effective method and used for the rapid capture and detection of clinical pathogen. The surface modification of nanoparticles was carried out using 3-aminopropyltriethoxy silane. The scanning electron microscopy image results showed the slightly agglomerated spherical shaped nanoparticles. Transmis-sion electron microscope result showed the polydispersed particles in the size ranges from 5 to 12 nm. The EDAX results confirmed the coating of silica with iron oxide particles. The SAED pattern confirmed the crystalline nature of iron oxide nanoparticles and also indicated the presence of silica. The FTIR spectrum of the nanoparticles confirmed the functional groups of the iron oxide and surface modified fluorescent silica coated iron oxide nano-particles (SFSIO). This work provides a very effective method for controlling the growth, capture and detection of pathogenic bacteria.

Variation in structure of needles of silver fir (Abies alba Mill.) saplings groowing under the canopies of diverse tree species

Robakowski P., Samardakiewicz S., Kierzkowski D.

Polish Journal of Ecology

2004

Vol. 52, nr 4

563-568

Morphological and anatomical traits of needles of Abies alba saplings growing under the canopies of Picea abies, Fagus sylvatica, Betula pendula, Pinus sylvestris and Larix decidua were studied. An acclimation of the needles to different light conditions created by the principal species of the canopy was assessed using different microscopic methods. Length, width, needle area, stomatal density, parenchyma thickness, resin ducts diameter, central cylinder size and other traits of needles allowed to determine their structural plasticity in response to irradiance level, Leaf mass to area ratio, palisadeparenchyma thickness, diameter of resin ducts increased, whereas width/thickness ratio and needle thickness/parenchyma thickness ratio decreased with increasing canopy openness. Variation in the investigated patterns of the needles reflected their structural plasticity and also an ability of silver fir saplings to acclimate to irradiance under the canopies of diverse tree species.