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1
Content available remote Advances in the application of biosynthesized carbon dots as fluorescent probes for bioimaging
Li Xuechan, He Jiefang
Materials Science Poland
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2024
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Vol. 42, No. 1
62--91
EN
Carbon dots (CDs) are emerging as versatile fluorescent nanoprobes for bioimaging applications due to advantages like tunable emissions, excellent biocompatibility, facile surface functionalization, and ease of synthesis. This review summarizes recent advances in applying biosynthesized CDs for sensitive bioimaging. CDs derived from sustainable biomass sources through green techniques like hydrothermal and microwave synthesis demonstrate bright, excitation-tunable photoluminescence spanning visible to near-infrared spectra. Careful control of synthesis parameters and surface passivation strategies enhance quantum yields above 50% comparable to toxic semiconductor dots. Conjugation with polymers, peptides, and recognition elements like antibodies impart solubility and selectivity towards cancer cells and biomarkers. In vitro validation in standard lines shows targeted organelle imaging abilities. In vivo administration reveals renal clearance pharmacokinetics with preferential tumor accumulation via enhanced permeability effects. Average tumor growth inhibition around 50-80% was achieved in mouse xenografts using CDs-drug formulations through combined therapeutic effects of chemotherapy and photothermal ablation under imaging guidance. However, concerns regarding toxicity from chronic exposures, large-scale reproducible manufacturing, and multimodal imaging capabilities need redressal prior to further clinical translation.
2
Układy diagnostyczne i źródła światła w fotodynamicznej metodzie terapii
Kwaśny M., Mierczyk Z., Łapiński M., Gietka A.
Elektronika : konstrukcje, technologie, zastosowania
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2014
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Vol. 55, nr 12
31-37
PL
Referat dotyczy metody fotodiagnostyki (PDD) i leczenia fotodynamicznego (PDT) i koncentruje się na terapeutycznych źródłach światła i systemach do wykrywania w organizmach fluoroforów oraz monitorowania parametrów metody PDD/PDT. Optoelektroniczne urządzenia stosowane w tej metodzie są wciąż ulepszane i adaptowane do pracy w warunkach klinicznych. W pracy zaprezentowano obecnie dostępne źródła światła do leczenia różnych ludzkich organów i nowe projekty aparatury dla fotodiagnostyki. Zaprezentowano również własne rozwiązania układów obrazowania i światłowodowych fluorescencyjnych analizatorów.
EN
The work on photodiagnosis (PDD) and photodynamic therapy (PDT) focuces on therapeutic light sources and diagnostic systems applicable to detection of fluorophores in an organism as well as to the monitoring of PDT parameters. Optoelectronics devices employed in this method are being still improved and adapted for applications in clinics. The paper presents currently available light sources used of various humans organs and new designs of photodiagnostic apparatus. Also, our new designs of imaging systems and fibre fluorescence analysers are showns.
3
Content available remote Time-resolved imaging of fluorescent inclusions in optically turbid medium : phantom study
Kacprzak M., Liebert A., Sawosz P., Żołek N., Milej D., Maniewski R.
Opto-Electronics Review
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2010
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Vol. 18, No. 1
37-47
EN
We present results of application of a time-resolved optical system for imaging of fluorescence excited in an inclusion containing indocyanine green (ICG), and located in optically turbid medium. The developed imaging system enabled simultaneous acquisition of fluorescence and diffusive reflectance. Eight independent time-resolved measurement channels based on time-correlated single photon counting technique were applied. In four of these channels, used for the fluorescence detection, sets of filters were applied in order to block the excitation light. Fast optomechanical switches allowed us to illuminate sequentially nine different spots on the surface of the studied object and finally 4×4 pixels maps at excitation and emission wavelengths were obtained. A liquid phantom used in this study consists of the fish tank filed with a solution ofmilk and water with black ink added to obtain optical properties in the range of the optical properties typical for the living tissue. A gel ball of a diameter of 5 mm with precisely controlled concentration of ICG was immersed in the liquid. The measurements were performed for inclusion located at different depths and for various ICG concentrations in the gel ball and in the surrounding liquid. The recorded distributions of times of arrival (DTA) of fluorescence photons and times of flight (DTOF) of diffusely reflected photons were analyzed by calculation of their statistical moments. We observed specific changes in moments of the measured DTAs as a function of depth of immersion of the fluorescent inclusion in the medium. We noted also that the changes of moments depend significantly on concentration of the dye in the fluorescence inclusion as well as in the surrounding liquid.
4
Content available remote Optical visualization of the heart conduction system
Venius J., Żurauskas E., Bagdonas S., Żurauskiene E., Rotomskis R.
Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
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2005
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Vol. 11, nr 3-4
6-9
EN
Optical diagnostic techniques nowadays are widely employed in many areas of medicine due to noninvasive approach and instant performance. Majority of these methods are based on the fluorescence measurements, which reflect molecular, biochemical and structural composition of the tissue. To meet the rising requirements of the heart surgery the up-to-date visualization of heart conduction system is needed. Surgeons confront with the problem such as how to do not harm the conduction system (HCS) during heart operations, which may result in dangerous heartbeat disturbances. In this study visualization experiments based on the tissue autofluorescence were performed ex vivo on various parts of the heart.
PL
Obecnie stosowane optyczne techniki diagnostyczne znajdują szerokie zastosowanie w wielu dziedzinach medycyny jako nieinwazyjne, przynoszące natychmiastowe rezultaty metody obrazowania medycznego. Większość z nich bazuje na pomiarach fluorescencji, które odzwierciedlają molekularny, biochemiczny oraz strukturalny skład tkanki. Aby sprostać wciąż wzrastającym wymaganiom stawianym podczas zabiegów operacyjnych serca, należy stosować nowoczesne systemy obrazowania układu przewodzenia w sercu. Chirurdzy wciąż napotykają problemy związane z uszkodzeniem układu przewodzenia (HCS) podczas przeprowadzanych operacji, co może prowadzić do poważnych zaburzeń pracy serca. W pracy przeprowadzono eksperymenty wizualizacji ex vivo różnych części serca, bazując na zjawisku autof luore-scencji tkanek.
5
Content available remote Optical visualization of the heart conduction system
Venius J., Żurauskas E., Bagdonas S., Żurauskiene E., Rotomskis R.
Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
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2004
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Vol. 10, nr 1-2
31-31
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