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Application of solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) and comparison of both detection techniques (DAD and FLD) to analyse nesfatin-1 in fetal bovine serum samples

Tuzimski Tomasz, Szubartowski Michał

Acta Chromatographica

2023

Vol. 35, no. 3

286--292

In this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography with both detection techniques such as diode-array detection and fluorescence detection (HPLC-DAD-FLD) for the determination of nesfatin-1 in fetal bovine serum samples. The limit of detection (LOD) and limit of quantification (LOQ) for nesfatin-1 were set at satisfactory values in the range 0.22–0.35 mg mL⁻¹ and in the range 0.67–1.05 mg mL⁻¹, respectively (at two different wavelengths (DAD) and at four different wavelengths (FLD)). Analyte concentrations were determined as the average value from fetal bovine serum matrix samples. The preliminary results show that the SPE procedure on Isolute Si-TsOH (SCX-3) could be used for further nesfatin-1 analyses in human serum samples. Both the SPE technique, chromatographic analysis with gradient elution mode and detection technique are fast and convenient.

Optical layout of the fluorescence detector of the Pierre Auger Observatory

Palatka M., Schovanek P., Hrabovsky M., Vlcek M., Ridky J., Grygar J., Soukup L., Prouza M., Bohacova M.

Optica Applicata

2003

Vol. 33, nr 2-3

445-456

We describe an unusual optical layout of the fluorescence detector telescope which is a part of the Pierre Auger Observatory. The fluorescence detector is a very fast telescope with the relative aperture fno ~ 0.77 and the large field of view 30×30 degrees. The primary objective for the Auger fluorescence telescope is to measure accurately the longitudinal development profiles of air showers of the cosmic rays above 10 EeV energy.