Wyniki wyszukiwania - BazTech

1

Physicochemical Characterization of Pomegranate (Punica Granatum L.) Native to Jordan During Different Maturity Stages: Color Evaluation Using the Cielab and Cielch Systems

Pérez Soraya Mercedes

Journal of Ecological Engineering

|

2021

|

Vol. 22, nr 6

214--221

EN

Punica granatum L. study is interesting for the functional properties associated to its Phenolic compounds and high volumes of production of this fruit in Jordan. The pomegranate type "pale arils" from Abu Ziyad valley were evaluated during different stages between 20 to 140 days after full bloom, with techniques of chelation, colorimetry and spectrophotometry. The results show that the increasing of sphericity (0.79 to 0.91), size diameter (65.58 to 90.41 mm), juice yield (46.03 to 55.67%), arils mass (31.70 to 61.23%), TSS total soluble solids (11.01 to 16.80), pH (2.7 to 3.6) and anthocyanins (7.43 to 12.41 Cyannidine 3 glucoside/100 g) were associated with the decreasing of thickness of the rind (4.9 to 2.77 mm), TTA Titrable total acidity (3.5 to 1.1%), TPC Total phenolics (389.61 to 251.52 mg Gallic acid/100 g), flavonoids (0.73 to 0.40 mg Catechine/g), tannins (8940.11 to 3324.79 ppm), AA Antioxidant Activity (63.41 to 48.84%) and Ascorbic acid content (28.91 to 9.48 mg/100 g); presenting significant differences p≥0,5. The paleness of rind and arils is positively related to the anthocyanin content expressed by "a" and "C" color coordinates. The actual investigation demonstrates that high values TSS-pH, low content of tannins and TTA could be successfully indicators of maturity stages rather than the concentration of anthocyanins.

2

Optical sensor of flavonoids based on liquid crystal

Sushynskyi Orest, Petrina Romana, Gubriy Zoriana, Khomyak Semen, Mykytyuk Zinoviy, Novikov Volodymyr

Informatyka, Automatyka, Pomiary w Gospodarce i Ochronie Środowiska

|

2019

|

T. 9, nr 1

61--64

EN

In order to solve the problems associated with the express registration of the presence of biologically active substances in callus biomass, we propose the use of cholesteric liquid crystal (CLC) with a spiral structure as a material of a sensitive element of optical sensor of flavonoids. Cholesteric liquid crystal BLO62 is proposed to use as a sensitive element. Spectral characteristics of alcohol solutions of flavonoids (20%, 40%, 70% and 90%) were obtained. The interaction of CLC with quercetin was analyzed. The scheme of the primary information signal converter is developed in which the current from the photodetector is transmitted to the developed signal converter. The device is implemented with the common use Atmega 328 microcontroller manufactured by Atmel. Speciality of this structural scheme is its flexibility and versatility, which makes it easy to reconfigure the parameters of the firmware for the registration of various types of investigated mediums.

PL

Aby rozwiązać problemy związane z szybką analizą obecności substancji biologicznie czynnych w biomasie kalusa, proponujemy zastosowanie materiałów ciekłokrystalicznych o spiralnej strukturze supramolekularnej jako wrażliwego elementu czujnika optycznego flawonoidów. Proponuje się stosowanie cholesterycznego ciekłego kryształu BLO62 jako wrażliwego elementu. Uzyskano charakterystykę spektralną roztworów alkoholowych flawonoidów (20%, 40%, 70% i 90%). Przeanalizowano interakcję cholesterycznego ciekłego kryształu z kwercetyną. Opracowano schemat pierwotnego przetwornika sygnału, w którym prąd z fotodetektora jest przesyłany do opracowanego przetwornika sygnałów. Urządzenie jest realizowane na bazie mikrokontrolera Atmega32 Atmega 328. Cechą tego schematu strukturalnego jest jego elastyczność i wszechstronność, co ułatwia rekonfigurację parametrów oprogramowania do rejestracji różnych typów badanych substancji.

3

Natural flavonoids as potential photosensitizers for dye-sensitized solar cells

Zdyb Agata, Krawczyk Stanisław

Ecological Chemistry and Engineering. S

|

2019

|

Vol. 26, nr 1

29--36

EN

Natural flavonoids quercetin, morin, fisetin and luteolin were studied as potential photosensitizers for dye-sensitized solar cells (DSSC). Spectroscopic methods were used to investigate the formation of dye/TiO2 nanoparticles assemblies and the development of their absorption spectra. The results show that the flavonoids adsorb well on TiO2 nanoparticles and this process causes the shift of absorption spectra from the near UV into the visible range of solar light. The mode of binding of the dye molecules on TiO2 surface is analyzed by comparison of spectral absorption properties and with the use of structural differences introduced by fisetin and luteolin for discrimination between several possibilities.

4

Comparison between conventional solid phase extraction and its simplified method for HPLC determination of five flavonoids in orange, tangerine, and lime juice samples

Asghari A., Barfi B., Barfi A., Saeidi I., Ghollasi-Moud F., Peyrovi M., Beig-Babaei A.

Acta Chromatographica

|

2014

|

Vol. 26, no. 1

157--175

EN

Although conventional solid phase extraction (SPE) is an applicable routine method for extraction of different analytes from various matrices, it requires more time and volume of solvent and sample rather than other routine laboratory extraction methods. In this study, a rapid and simplified sample preparation method based on SPE was studied by eliminating some steps, such as conditioning and washing, for extraction of diosmin, eriocitrin, narirutin, naringin, and hesperidin in orange, tangerine, and lime juice samples. The separation of these flavonoids was achieved by using a C8 column with a mobile phase comprised of water-acetonitrile-acetic acid (78:21:1, v/v/v) at a flow rate of 0.85 mL min-1 and UV detection at 280 nm. To examine the applicability of this method, effective parameters such as type of adsorbent, type and volume of elution solvent, ionic strength, and pH of the sample were studied and applied to the comparison between the conventional SPE and the simplified method. The best recoveries were obtained, using the proposed method, with a small volume of citrus fruit juice (0.5 mL), silica gel (0.5 g) as adsorbent, and 3 mL of methanol as elution solvent. Limits of detection, limits of quantification, intra-day and inter-day precision of the method for the analytes were 0.0244–0.0587 μg mL-1, 0.0739–0.178 μg mL-1, 2.5 3.1%, and 3.1–4.8%, respectively.

5

Quality evaluation of Yanghuo Sanqi tablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

Zhu F. X., Jia X. B., Cai Y., Chen Y.

Acta Chromatographica

|

2013

|

Vol. 25, no. 1

147--157

EN

Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography-diode array detector-evaporative light-scattering detector (HPLC-DAD-ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile-water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min-1. The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC-DAD-ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

6

Simultaneous determination of nine flavonoids in beagle dog by HPLC with DAD and application of Ginkgo biloba extracts on the pharmacokinetic

Wu J., Xing H, Tang D., Gao Y., Yin X, Du Q., Jiang X., Yang D.

Acta Chromatographica

|

2012

|

Vol. 24, no. 4

627--642

EN

The method of high-performance liquid chromatography (HPLC) with diode array detector (DAD) was used and validated for the simultaneous determination of nine flavonoids (rutin, myricetin, quercitrin, quercetin, luteolin, genistein, kaempferol, apigenin, and isorhamnetin) in beagle dog plasma. Plasma sample was pre-treated with acetonitrile (containing 0.05% formic acid). Chromatographic separation was performed on a kromasil C18 column (250 × 4.6 mm, 5 µm) maintained at 35 °C. The mobile phase was a mixture of methanol and 0.2% formic acid with a step linear gradient. At 1.0 mL min-1 flow rate, the eluent of other eight flavonoids was detected simultaneously at 360 nm with good separation except genistein (detected at 254 nm). Under optimum conditions, the correlation coefficient between the peak area and the concentrations for each analyte was all above 0.999. The intra-day and inter-day precisions were less than 10% for all analytes. The limit of detection and the limit of quantification for the selected nine flavonoids were 0.006–0.03 and 0.02–0.12 g mL, -1 respectively. The extracted recoveries of selected nine flavonoids were 74.02%–99.37%. The assay has been successfully applied to determine concentrations of nine flavonoids in plasma from beagle dog after being intravenously administrated Ginkgo biloba extract.

7

Quality evaluation of Yanghuo Sanqi tablet through a simultaneous determination of five major active Flavonoids and three main saponins by HPLC-DAD-ELSD

Zhu F. X, Jia X. B, Cai Y., Chen Y.

Acta Chromatographica

|

2012

|

Vol. 24, no. 4

667--677

EN

Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of Herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography-diode array detector-evaporative light scattering detector (HPLC-DAD-ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile-water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min-1. The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC-DAD-ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

8

Analysis of Saussurea species from tibet using HPLC-DAD-ESI-MS n

Dawa Z, Zhou Y., Bai Y., Gesang S, Liang J., Ding L.

Acta Chromatographica

|

2010

|

Vol. 22, no. 1

141--152

EN

An HPLC-DAD-ESI-MS n method has been developed for simultaneous quantification of eight major compounds in eight Saussurea species which have long been used as the traditional Tibetan medicines. The method was validated for sensitivity, precision, and accuracy. LODs were from 0.11 to 5.01 μg mL -1, overall intra-day and inter-day variation was less than 2.70%, and overall recovery was over 98.0%. The correlation coefficients ( r 2) of the calibration plots were >0.991. This newly established method was successfully used to reveal difference among the chemical profiles and analytes contents of eight Saussurea species collected in Tibet. In addition, by comparison of UV and mass spectra with those of authentic compounds, a total of fifteen peaks were identified. It can be concluded that this is an effective method for quantification and evaluation of the flavonoids and coumarins in the eight species of the genus Saussurea . It can be used as an efficient reference method for development and use of the eight traditional Tibetan medicines by comparing their different characteristics.

9

Hollow fiber-based liquid phase microextraction combined with high performance liquid chromatography for determination of flavonoids in Ginkgo biloba and urine

Lu H., Wang X., Liu X., Jiang S.

Chemia Analityczna

|

2009

|

Vol. 54, No. 3

309-319

EN

A hollow fibre membrane-based liquid phase microextraction (HF-LPME) method for determination of flavonoids in Ginkgo biloba leaves was developed. Three flavonoids: quercetin, kaempferol, and isorhamnetin were extracted and then analysed by high performance •-:; liquid chromatography. The factors affecting HF-LPME, including concentration of donor ,; and acceptor phases, concentration of NaCl, extraction time, and stirring speed were systematically investigated. Under the optimum conditions, calibration plots of reasonable linearity were constructed in the analyte's concentration range of 10-100 μg L-1. Up to 200-fold enrichment factor for quercetin and up to tens of folds for kaempferol and isorhamnetin ' were obtained. The method was applied to determine the concentrations of flavonoids in urine and was proven to be an convenient sample preparation technique.

PL

Opracowano metodę oznaczania flawonoidów w liściach Ginkgo biloba za pomocą ^ mikroekstrakcji z fazy ciekłej przy użyciu membranowego wydrążonego włókna. Ekstra-howano i analizowano trzy flawonoidy: kwercetynę, kempferol i izorhamnetynę stosując wysokosprawną chromatografię cieczową. Systematycznie zbadano następujące czynniki wpływające na mikroekstrakcję: stężenie donora i akceptora, stężenie NaCl, czas ekstrakcji oraz szybkość mieszania. W optymalnych warunkach uzyskano liniowy wykres krzywej kalibracji w zakresie stężenia analitu od l O do 100 μg L-1. Współczynnik zatężenia wynosił do 200 razy w przypadku kwercetyny i do dziesiątek razy w przypadku kempferolu oraz izorhamnetyny. Opracowaną metodę zastosowano do oznaczania zawartości flawonoidów w moczu. Stwierdzono, że metoda jest odpowiednia do przygotowywania próbek.

10

Lignans and Flavonoids from the Seeds of Centaurea bornmuelleri Hausskn. Ex. Bornm and Centaurea huber-morathiiWagenitz.

Shoeb A., MacManus S., Jaspars M., Nahar L., Kong-Thoo-Lin P., Celik S., Sarker S.

Polish Journal of Chemistry

|

2007

|

Vol. 81, nr 1

39-44

EN

The reversed-phase preparative HPLC purification of the methanol extracts of the seeds of two Turkish endemic species Centaurea bornmuelleri and Centaurea huber-morathii afforded several dibenzylbutyrolactone-type lignans and flavonoids. The lignans arctiin, matairesinol and matairesinoside, and the flavonoid, afzalin, were found in both species. While arctigenin and astragalin were only present in C. bornmuelleri, kaempferol was isolated from huber-morathii. Aplant sterol, stigmast-4-en-3gamma-ol, was also isolated from the dichloromethane extract of C. bornmuelleri. The structures of the isolated compounds were determined by spectroscopic means. The chemotaxonomic significance of these compounds within the genus Centaurea has been discussed.