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1 2009

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A simple and reliable preparative high-performance liquid chromatographic technique for isolation of a bioactive flavone diglycoside from and extract of Cuminum cyminum seeds

Satti N. K, Amina M, Dutt P, Sharma V. K., Sharma P., Khan I., Gupta B. D, Suri K. A, Sharma S. C., Johri R. K, Sharma S. N

Acta Chromatographica

2009

Vol. 21, no. 3

499--512

In this paper we describe a sensitive and reproducible reversed-phase high-performance liquid chromatography (HPLC) method with photodiode-array detection for isolation and quantification of the bioactive hydrophilic constituent 7-(1- O -β-D -galacturonide-4′-(1- O -β-D -glucopyranosyl)-3′,4′,5,7-tetrahydroxyflavone, 1 , from the seeds of Cuminum cyminum . Compound 1 was separated isocratically on a C 18 preparative column, in high purity, after removal of solvents. The purity and identity of the compound were established by use of LC-mass spectrometry and by spectroscopic techniques ( 1 H and 13 C NMR). The purity of 1 was also confirmed by HPTLC.