In this study, the collected fish species were identified based on morphological observations and then evaluated by DNA barcoding. The COI gene has been recognized as a biological marker for fish species identification and the objective of this study is to analyze the variable region of the COI gene in subunit I. The mitochondrial cytochrome (COI) oxidase subunit I gene was analyzed as a suitable molecular maker for the identification of three specimens of the fish species Hipposcarus harid, widely distributed in the Red Sea. The COI gene sequences in the variable region revealed variations among the fish species. The COI gene sequences in the variable region were similar to the variable region of Hipposcarus harid collected from the Northern Red Sea and all three were named: H. harid H13, H. harid H2c, and H. harid H12. The identification of the fish species collected from the Red Sea in Saudi Arabia would help ichthyologists improve the management, conservation and monitoring of economically important long-nose parrotfish species in Saudi Arabia.
Hydroacoustical measurements of fish population in the shallow Malta reservoir (mean depth <3 m) were performed in September and October 2008 using mobile horizontal beam. During the different time of day and night the same transects were covered in two directions, from the dam towards the upper part of the reservoir and back. During the day the number of fish registered on the returned way was lower than on the way forward in 83% of cases, and during the night in 67% of cases, however these differences were not statistically significant. Observation of fish at different distances from the boat have shown small avoidance reaction, especially for the small fish, that was similar during the day and at night, thus suggesting that mainly noise was responsible for the fish avoidance. Nevertheless, in shallow waters boat avoidance by fishes is of much less importance than in marine environment.
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