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Human endothelium on vascular prostheses modified by extracellular matrix proteins in a flow experiment

Chlupac J., Filova E., Riedel T., Brynda E., Remy-Zolghadri M., Bareille R., Fernandez P., Daculsi R., Bordenave L., Bacakova L.

Inżynieria Biomateriałów

2006

R. 9, nr 58-60

10-13

Artificial vascular prostheses are used for bypass surgery. Thrombogenicity often cause graft occlusion. Targeted surface alterations including cell seeding may improve the haemocompatibility. Knitted commercial tubular PET (polyethylene terephtalate) vascular prostheses with collagen impregnation were modified by adsorption of laminin (LM) or coating with fibrin network (FB) on the luminal surface. Human endothelial cells were harvested, cultured and seeded at the density of 150x10\3 cells/cm square on all grafts. The cell lining was continuously visualized and quantified. The retention was 21%, 37% and only 2% of the seeding density on the unmodified (UM), LM- and FB-coated grafts, respectively. These seeded prostheses were exposed to a laminar shear stress (SS) 15 dynes/cm square for 40 minutes (UM, LM, FB) and 120 min (UM, LM) in a chamber simulating blood circulation. The SS was excluded in static (ST) control grafts. After 40 min-SS the cell numbers were78%, 27% and 72% for the UM, LM and FB prosthesis compared to the ST. The cell densities were 61% and 57% on the UM and LM after 120 min-SS. To conclude, the endothelium formed a confluent layer whereas laminin immobilization improved endothelial adhesion but not the flow resistance. Reverse effect was observed on fibrin coating.

Niskocząsteczkowe inhibitory plazminy

Midura-Nowaczek K.

Wiadomości Chemiczne

1998

[Z] 52, 11-12

871--887

Plasmin, serine protease with trypsin specifity, plays a key role in the process of fibrinolysis. Analogs of lysine like e-aminocaproic acid and trans-4-aminomethylclohexanecarboxylic acid are employed clinically as plasmin inhibitors and exhibit an inhibitory effect by blocking lysine binding sites of the enzyme. Their inhibitory activity on plasmin with the respect to fibrinogen and other proteins is much weaker than towards fibrin. Because of this reason investigations on the synthesis of an active centre directed inhibitors of plasmin were also undertaken. A great number of compounds was obtained including derivatives of w-aminoacids or lysine. Next important group were short peptides with C-terminal lysine or arginine derivatives such as aldehydes, chloromethylketones, fluoromethylketone, methylketones, amides or esters. This paper presents literature data on structure-activity relationship for low molecular inhibitors of plasmin with a structure of aminoacids or peptides derivatives.