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EN
This study aimed to produce gellan gum-based hydrogels with the addition of zinc oxide as a potential dressing material. Hydrogels with ZnO concentrations of 0.01%, 0.02% and 0.04% were prepared, micrometric and nanometric ZnO particles were used, and a CaCl2 crosslinker was added to one part of the samples. All samples (14 types) produced by the freeze drying method were characterized with high swelling properties (>2000%), what is important to ensure the absorption of exudates from wounds. Samples with ZnO particles cross-linked with CaCl2 lost less mass after incubation in aqueous media and were characterized by better dimensional stability than those without crosslinking. The pH of the extracts of the samples containing ZnO particles was more neutral (pH 7.0-7.6) than that of the control gellan gum samples (pH of 5.5-6.1). The zinc release from cross-linked samples was twice as high for those containing nanometric particles than for micrometric particles (1.94 ± 0.04 mg/l and 0.93 ± 0.02, respectively). Relatively large amounts of released zinc species in the case of samples containing ZnO nanoparticles are promising in the context of the antibacterial properties and treatment of infected wounds. A lower amount of zinc released from samples with ZnO microparticles could be sufficient to prevent the development of the infection. Furthermore, both materials show satisfactory cytocompatibility with L929 fibroblasts, as shown by Alamar blue and live/dead viability tests, making them prospective candidates for wound healing
PL
Zbadano wpływ dodatku ciekłych prekursorów porów na morfologię, porowatość i właściwości mechaniczne polilaktydowych rusztowań komórkowych. Rusztowania otrzymano metodą mokrej inwersji faz w wariancie freeze extraction. Oceniono cytotoksyczność wybranych rusztowań w stosunku do fibroblastów mysich oraz ich przydatność do hodowli komórkowych. Wykazano, że dodatek prekursora porów dopolilaktydu korzystnie zmienia morfologię wytworzonych rusztowań, jednocześnie pogarszając ich wytrzymałość mechaniczną. Stwierdzono, że polilaktydowe rusztowania komórkowe z powodzeniem mogą być wykorzystywane do hodowli komórkowych.
EN
The effect of liquid pore precursor addition on the morphology, porosity and mechanical properties of polylactide scaffolds was investigated. The scaffolds were obtained by inversion phase method in freeze extraction mode. Selected scaffolds were subjected to a cytotoxicity test using mouse fibroblastcells. It has been shown that the addition of pore precursors favorably changes the morphology of scaffolds at the cost of decreased mechanical strength. It has been found that polylactide cellular scaffolds can be successfully used forcell culture.
EN
Strategies to improve healing of damaged nerves include the application of specialized nerve guides, which hold the promise for allowing reanastomosis of the severed or damaged fibers. Studies have demonstrated that the use of a slowly degradable polymeric nerve guide can improve the nature and rate of nerve regeneration across a short gap in small nerves. The objective of this study was to characterize a biodegradable nerve guide based on poly(trimethylene carbonate-co-lactide) for peripheral nerve regeneration and to evaluate its cytotoxicity. The obtained copolymer films were incubated in two different media (distilled water and simulated body fluid), and while the degradation process appeared, pH and ion conductivity changes of solutions were monitored as well as mass loss of the samples. Additionally, mechanical tests (tensile strength, elongation at break and Young’s modulus parameters) before and after different time points were carried out. To evaluate cytotoxicity biological test were done on fibroblasts cells (NIH 3T3). Cell metabolic activity was determined using Alamar Blue reagent and their morphology was observed under fluorescence microscopy. The growth of pH in both media were mostly caused by steadily degradation of carbonate units into alkaline diols. The growth of ion conductivity value at the beginning of the incubation process was associated with the releasing of free ions to the solution. The mechanical parameters decreased with the progress of degradation process. Ringer’s fluid, as more aggressive, caused higher decrease in mechanical properties. The measured contact angles showed good surface wettability. Both surfaces, the top and the bottom, had similar hydrophilicity. Moreover, activity of fibroblasts cells were similar on both sides as well as on the reference TCPS. Good adhesion of NIH 3T3 cells to the surface suggests that the hydrophilic polymers promote colonization of fibroblasts cells on their surface. Biological studies have shown that used cells are very sensitive to surface topography which they colonize and cell viability was higher at the bottom surface, which has a slightly higher average roughness Ra. Thus, fibroblasts cell preferred colonizing rougher than smoother surfaces. Fabricated films does not affect negatively, namely, toxic on cell cultures and forms substrate with favourable surface properties. This was confirmed by the Alamar Blue tests and microscopic observations.
PL
Karetka pogotowia transportująca pacjentów z chorobami zakaźnymi często może być źródłem kolejnych infekcji. Powodem może być m.in jej specjalna konstrukcja - wąska przestrzeń wewnątrz pojazdu, zbyt słaby system wentylacji oraz specyfika znajdujących się tam urządzeń. Problem ten jest dosyć dobrze znany, ale niewiele danych dotyczy wpływu stanu powierzchni wewnętrznych karetki. Badania były zogniskowane na toksyczności i biozgodności stalowych powierzchni pokrytych różnymi powłokami, które mogą być wykorzystane jako materiał wewnętrznego poszycia pojazdu medycznego.
EN
Ambulance transporting patients with infectious diseases can be also the source of the next infections. Special construction - the narrow space inside, pure quality ventilation system and specific of devices applied inside can be a reason of mentioned problems. This situation is well known, but there is practically no data regarding the influence of internal ambulance surface on observed problem. In our study we focused on toxicity and biocompatibility of steel surface coated with different layers, that can be used as internal material of ambulance.
PL
Metodą dającą duże nadzieje na ograniczenie zjawiska restenozy, jako najczęstszego odległego powikłania zabiegów przezskórnej angioplastyki wewnątrznaczyniowej, jest terapia fotodynamiczna (PDT). Obserwowano hamujący wpływ PDT na komórki ściany naczyniowej uczestniczące w procesie restenozy. W poniższym eksperymencie badano oddziaływanie terapii fotodynamicznej z użyciem chlorinu E6 na fibroblasty linii NIH3T3.
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