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1
Content available Development of a new production method of foam-like wound dressings for skin regeneration
Vivcharenko Vladyslav, Kazimierczak Paulina, Wójcik Michał, Przekora Agata
Engineering of Biomaterials
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2019
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Vol. 22, no. 152
16--20
EN
Chitosan is widely used to prepare films, hydro-gels, cryogels, sponges, fibers and other various biomaterials used in the tissue engineering field. It is one of the best processable polysaccharides used in biomedicine. However, its stability is generally lower as compared with others, due to its pH sensitivity and hydrophilic character. Using chitosan in combination with agarose may not only improve chemical and mechanical properties of the resultant material (by the formation of a biocomposite), but also lead to the formation of a gel imitating physical attributes of the extracellular matrix. Moreover, the combination of these two polysaccharides has a promising ability to improve the stability of chitosan and to increase fibroblasts’ affinity to agarose. Characteristic advan-tageous features of these natural polymers raise a wide interest in tissue engineering. The aim of this study was to develop and optimize a new method to produce a highly biocompatible foam-like chitosan/agarose wound dressing for skin healing applications. The production process optimization helped to obtain the absorbent foam-like biomaterial which is non-toxic to skin fibroblasts and does not conduce their adhesion. Employing sodium bicarbonate as the main agent in the foaming reaction not only led to obtaining the foam-like structure but also neutralized the acidic pH, making the material non-toxic and non-irritating to the skin. In conclusion, the new foam-like biomaterial has great potential for biomedical applications as the wound dressing accelerating the healing process of the damaged tissues.
2
Content available Ocena właściwości użytkowych rusztowań komórkowych o strukturze gąbczastej oraz wzrostu na nich fibroblastów
Kruk A., Gadomska-Gajadhur A., Dulnik J., Rykaczewska I., Ruśkowski P., Sebai A., Synoradzki L.
Polimery
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2018
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T. 63, nr 4
270--274
PL
Zbadano wpływ dodatku ciekłych prekursorów porów na morfologię, porowatość i właściwości mechaniczne polilaktydowych rusztowań komórkowych. Rusztowania otrzymano metodą mokrej inwersji faz w wariancie freeze extraction. Oceniono cytotoksyczność wybranych rusztowań w stosunku do fibroblastów mysich oraz ich przydatność do hodowli komórkowych. Wykazano, że dodatek prekursora porów dopolilaktydu korzystnie zmienia morfologię wytworzonych rusztowań, jednocześnie pogarszając ich wytrzymałość mechaniczną. Stwierdzono, że polilaktydowe rusztowania komórkowe z powodzeniem mogą być wykorzystywane do hodowli komórkowych.
EN
The effect of liquid pore precursor addition on the morphology, porosity and mechanical properties of polylactide scaffolds was investigated. The scaffolds were obtained by inversion phase method in freeze extraction mode. Selected scaffolds were subjected to a cytotoxicity test using mouse fibroblastcells. It has been shown that the addition of pore precursors favorably changes the morphology of scaffolds at the cost of decreased mechanical strength. It has been found that polylactide cellular scaffolds can be successfully used forcell culture.
3
Content available Fabrication, properties and cytotoxicity evaluation of degradable poly(trimethylene carbonate-co-lactide) for the use as nerve guidance channels
Bednarz P.
Tarnowskie Colloquia Naukowe. Nauki Techniczne i Ścisłe
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2017
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T. 4, nr 3
39--48
EN
Strategies to improve healing of damaged nerves include the application of specialized nerve guides, which hold the promise for allowing reanastomosis of the severed or damaged fibers. Studies have demonstrated that the use of a slowly degradable polymeric nerve guide can improve the nature and rate of nerve regeneration across a short gap in small nerves. The objective of this study was to characterize a biodegradable nerve guide based on poly(trimethylene carbonate-co-lactide) for peripheral nerve regeneration and to evaluate its cytotoxicity. The obtained copolymer films were incubated in two different media (distilled water and simulated body fluid), and while the degradation process appeared, pH and ion conductivity changes of solutions were monitored as well as mass loss of the samples. Additionally, mechanical tests (tensile strength, elongation at break and Young’s modulus parameters) before and after different time points were carried out. To evaluate cytotoxicity biological test were done on fibroblasts cells (NIH 3T3). Cell metabolic activity was determined using Alamar Blue reagent and their morphology was observed under fluorescence microscopy. The growth of pH in both media were mostly caused by steadily degradation of carbonate units into alkaline diols. The growth of ion conductivity value at the beginning of the incubation process was associated with the releasing of free ions to the solution. The mechanical parameters decreased with the progress of degradation process. Ringer’s fluid, as more aggressive, caused higher decrease in mechanical properties. The measured contact angles showed good surface wettability. Both surfaces, the top and the bottom, had similar hydrophilicity. Moreover, activity of fibroblasts cells were similar on both sides as well as on the reference TCPS. Good adhesion of NIH 3T3 cells to the surface suggests that the hydrophilic polymers promote colonization of fibroblasts cells on their surface. Biological studies have shown that used cells are very sensitive to surface topography which they colonize and cell viability was higher at the bottom surface, which has a slightly higher average roughness Ra. Thus, fibroblasts cell preferred colonizing rougher than smoother surfaces. Fabricated films does not affect negatively, namely, toxic on cell cultures and forms substrate with favourable surface properties. This was confirmed by the Alamar Blue tests and microscopic observations.
4
Content available Effect of fibrin-nanocoating of nanofibrous polymer membranes on the adhesion and proliferation of human dermal fibroblasts
Pajorova J., Bacakova M., Musilkova J., Stranska D., Riedel T., Bacakova L.
Engineering of Biomaterials
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2016
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Vol. 19, no. 138 spec. iss.
48
5
Content available Toksyczność i biokompatybilność wybranych powłok lakierniczych
Hajduga M. A., Hajduga M., Sołek D., Jędrzejczyk D.
Aktualne Problemy Biomechaniki
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2014
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z. 8
45--50
PL
Karetka pogotowia transportująca pacjentów z chorobami zakaźnymi często może być źródłem kolejnych infekcji. Powodem może być m.in jej specjalna konstrukcja - wąska przestrzeń wewnątrz pojazdu, zbyt słaby system wentylacji oraz specyfika znajdujących się tam urządzeń. Problem ten jest dosyć dobrze znany, ale niewiele danych dotyczy wpływu stanu powierzchni wewnętrznych karetki. Badania były zogniskowane na toksyczności i biozgodności stalowych powierzchni pokrytych różnymi powłokami, które mogą być wykorzystane jako materiał wewnętrznego poszycia pojazdu medycznego.
EN
Ambulance transporting patients with infectious diseases can be also the source of the next infections. Special construction - the narrow space inside, pure quality ventilation system and specific of devices applied inside can be a reason of mentioned problems. This situation is well known, but there is practically no data regarding the influence of internal ambulance surface on observed problem. In our study we focused on toxicity and biocompatibility of steel surface coated with different layers, that can be used as internal material of ambulance.
6
Content available Influence of surface topography and morphology of PU/PLA films on fibroblasts performance
Bednarz P., Krok M., Laska J.
Engineering of Biomaterials
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2012
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Vol. 15, no. 116-117 spec. iss.
120--122
7
Content available Polymer carriers modified by plasma and functionalized with Au nanoparticles as substrates for mouse 3T3 fibroblasts
Bacakova M., Makajova Z., Slepickova-Kasalkova N., Svorcik V., Bacakova L.
Engineering of Biomaterials
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2011
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Vol. 14, no. 109-111 spec. iss.
5
EN
Polymers have been o f ten applied in biology and medicine for construction of tissue replacements. However, the inert surface o f the most polymers is not able to support and control cell adhesion, migration, proliferation, differentiation and other cell functions. Hence, the modification of polymer surface led to achieve appropriate properties. The polymer surface can be modified by plasma discharge by which the polymer surface chemistry and morphology is changed. Plasma treatment leads to creation of radicals, unsaturated bonds and new chemical groups, mainly oxygen containing groups. Oxidized groups increase the wettability of polymers, which supports adsorption of cell adhesion-mediating extracellular matrix (ECM) molecules in appropriate spatial conformation increasing accessibility of specific sites in these molecules by cell adhesion receptors. In addition, other surface properties of polymers are altered by plasma etching which strongly influence cell-material interaction. Radicals and unsaturated chemical bonds which are created by plasma can be utilized for grafting new chemical groups, biomolecules and nanoparticles. The biomolecules grafted on the polymer surface, such as amino acids, RGD-containing oligopeptides (i.e., ligands for integrin receptors), ECM molecules, enazymes, hormones, and also carbon and gold nanoparticles, not only have specific biological effects on cells but also change physical and chemical properties of the polymer surface, and by this way they support its bioactivity. This study is focused on physiochemical properties and biocompatibility of modified polymers. The studied materials were poly(L-lactide) (PLLA) foils, nanofibrous PLLA meshes and polyethylene terephtalate (PTFE) foils. PLLA and PTFE foils were modified in plasma with Ar + ions for time intervals of 50, 10 0 and 300 s with power 8 W, and then grafted with Au nanoparticles. Changes in the surface wettability were determined by reflection goniometry . The presence an d concentration o f Au nanoparticles were examine d by X-ray Photoelectron Spectroscopy (XPS). For the biocompatibility testing, the polymers were seeded by mouse embryonic fibroblasts of the line 3T3, i.e., t he cells of ten utilized as a feeder for keratinocytes. The cell adhesion and growth was evaluated by the number of cells, their morphology and the size of cell adhesion area in the 1st, 3rd and 6th day after seeding. The results indicate that the water drop contact angle increases with the time of exposure to plasma, which means that the vettability decreases. However, the following exposure of plasma-irradiated polymers to a sodium citrate solution (i.e., a storage solution for Au nanoparticles) and grafting with Au nanoparticles decrease the contact angle, i.e., increase the material surface wettability. Our tests of biocompatibility indicate that the modification of the polymer surface in fluences positively the cell behavior. The cells adhered at higher numbers and by a larger cell adhesion area on modified polymers; it was mainly manifested on PTFE.
8
Content available Fibroblast biological activity on poly(l-lactide) and poly(l-lactide-co-trimethylene carbonate)
Ścisłowska-Czarnecka A., Pamuła E., Kołaczkowska E.
Engineering of Biomaterials
|
2011
|
Vol. 14, no. 102
7--10
EN
Poly-L-lactide (PLLA) is acknowledged biocompatible polyester. However, it possesses high crystallinity/brittleness/stiffness and requires long time for complete degradation. In the current study we present data on PLTMC, a copolymer of L-lactide and trimethylene carbonate (TMC). Poly(trimethylene carbonate) (PTMC) is characterised by good mechanical properties and rapid degradation rate and for this it might possess new desired features for medical applications. During the experiments, adhesion and activity of fibroblasts cultured on PLLA and PLTMC were studied and compared during two time points of 3 and 5 days. On day 3, the number of adherent fibroblasts was compromised when fibroblasts were cultured in the presence of PLTMC but the proper adherence was recovered by day 5. The same pat-tern was observed when we evaluated some activity parameters of fibroblasts. In particular, the release of proteins and nitric oxide was studied as the increased levels of the mediators might indicate unwanted inflammatory-like condition. Overall, the results suggest that the synthesized PLTMC initially shows unwanted effects on fibroblasts but with the time these effects are abolished. Therefore PLTMC seems to represent a new material that is non-cytotoxic and compatible with the living cells.
9
Content available Wpływ biomateriałów na przyleganie i aktywność mysich fibroblastów L929
Ścisłowska-Czarnecka A., Pamuła E., Płytycz B., Kołaczkowska E.
Engineering of Biomaterials
|
2008
|
Vol. 11, no. 81-84
83--86
10
Content available Ocena biozgodności bioresorbowalnych materiałów polimerowych wobec ludzkich fibroblastów
Orchel A., Jelonek K., Kasperczyk J., Dobrzyński P., Orchel J., Dzierżewicz Z.
Engineering of Biomaterials
|
2008
|
Vol. 11, no. 81-84
5--8
11
Content available Modyfikacja chemiczna kopolimeru glikolidu z epsylon-kaprolaktonem i jej wpływ na adhezję i żywotność fibroblastów in vitro
Pamuła E., Ścisłowska-Czarnecka A., Szlęk A., Chadzińska M., Dobrzyński P., Płytycz B.
Inżynieria Biomateriałów
|
2006
|
R. 9, nr 58-60
24-28
PL
W celu poprawienia biozgodności kopolimeru glikolidu z epsylon-kaprolaktonem (PGCap) został on zmodyfikowany poprzez inkubację przez różne okresy czasu w roztworze wodnym zasady sodowej. Zastosowana modyfikacja nie wpłynęła na chropowatość powierzchni lecz spowodowała wytworzenie tlenowych grup funkcyjnych, które nadały powierzchni charakter polarny i spowodowały obniżenie hydrofobowości. Badania in vitro wykazały lepszą adhezję i żywotność fibroblastów (L929) hodowanych na próbkach modyfikowanych powierzchniowo.
EN
Copolymer of glycolide and epsylon-caprolactone was modified by incubation in aqueous solution of sodium hydroxide for different periods of time in order to improve its biocompatibility. Applied modification did not influence surface roughness but created oxygenated functions, which enhance surface polarity and diminish hydrophobicity. In vitro studies showed better adherence and viability of fibroblasts (L929) cultivated on surface-modified samples.
12
Content available remote Terapia fotodynamiczna z zastosowaniem chlorinu E6 indukuje proces apoptozy fibroblastów
Biały D., Wawrzyńska M., Arkowski J., Nowosad H., Mazurek W., Zioło E., Strządała L.
Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
|
2006
|
Vol. 12, nr 2
117-119
PL
Metodą dającą duże nadzieje na ograniczenie zjawiska restenozy, jako najczęstszego odległego powikłania zabiegów przezskórnej angioplastyki wewnątrznaczyniowej, jest terapia fotodynamiczna (PDT). Obserwowano hamujący wpływ PDT na komórki ściany naczyniowej uczestniczące w procesie restenozy. W poniższym eksperymencie badano oddziaływanie terapii fotodynamicznej z użyciem chlorinu E6 na fibroblasty linii NIH3T3.
13
Content available The influence of fractionated radiation on proliferation, cell cycle and apoptosis of normal human dermal fibroblasts
Adamczyk A., Gasińska A.
Nukleonika
|
2005
|
Vol. 50,suppl.2
9-12
EN
The aim of the study was to investigate the changes in proliferation rate, cell cycle and apoptosis of normal skin fibroblasts during fractionated irradiation with a fraction dose of 2 Gy. Fibroblasts were irradiated 5 days per week for 12 days using gamma irradiation. Twenty four hours after each fraction, and for three days after finishing experiment the cells were harvested, fixed, and BrdUrd labelling index (BrdUrdLI), cell cycle and level of apoptosis and debris were assessed. It was found that fractionated irradiation caused disturbances in the proliferation rate and the cell cycle. Irradiation caused also constant, statistically significant increase in the number of G2M cells and level of apoptosis and debris, which was observed even during 3 days after irradiation. Data indicate non equal biological effect of each fraction dose. Block at G2/M phase suggests accumulation of sublethal damage and increased radiosensitivity, which was manifested by elevated level of cell death (apoptosis and debris).
14
Content available Zmiany pojemności antyoksydacyjnej fibroblastów po kontakcie z wybranymi biomateriałami
Majak J., Jakubowski W., Balcerczyk A., Niedzielski P., Walkowiak B.
Inżynieria Biomateriałów
|
2005
|
R. 8, nr 43-44
63--66
15
Content available Ocena wpływu wybranych materiałów ceramicznych na fibroblasty i osteoblasty w hodowli in vitro
Chróścicka A., Woźniak P., Olkowski R., Lewandowska-Szumieł M., Michałowski S., Jaegermann Z., Karaś J.
Inżynieria Biomateriałów
|
2004
|
R. 7, nr 38-42
39-41
16
Content available Wpływ sterylizacji na adhezję ludzkich fibroblastów do biomateriałów
Sowińska A., Cukrowska B., Czarnowska E.
Inżynieria Biomateriałów
|
2002
|
R. 5, nr 23-25
15-17
17
Content available Oddziaływanie fibroblastów, osteoblastów i makrofagów z chirurgicznymi siatkami polipropylenowymi
Czajkowska B., Kowal J., Ptak M., Bobek M., Sobek C.
Inżynieria Biomateriałów
|
2002
|
R. 5, nr 23-25
42-43
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