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Comparative study of phenol degradation with a wild-type and genetically modified P. vesicularis (pBR322). plasmid stability and fame profiling

Mrozik A., Swędzioł Ż., Miga S.

Environment Protection Engineering

2015

Vol. 41, nr 1

137--155

It was evidenced that P. vesicularis is an efficient degrader of phenol but does not have a reporter system for monitoring bacterial survival in the environment. Therefore, P. vesicularis (pBR322) has been constructed. In this study we experimentally confirmed that introduction of plasmid pBR322 into P. vesicularis did not change its ability to degrade phenol in liquid media and after its inoculation into sterile soil. Moreover, it has been shown that plasmid pBR322 was stable in P. vesicularis during all experiments. Additionally, the pattern of fatty acid methyl esters for P. vesicularis (pBR322) looked similar to that of P. vesicularis under phenol exposure. Some fatty acids, especially branched and cyclopropane ones were sensitive markers of phenol utilization. These findings indicate that P. vesicularis (pBR322) due to the presence of plasmid could be used instead of P. vesicularis in bioaugmentation of phenol-contaminated areas.