Wyniki wyszukiwania - BazTech

1

Immobilizacja enzymów na nośnikach sposobem na ukierunkowaną modyfikację właściwości biokatalizatorów

Bolibok P., Gembala J., Wujak M., Roszek K., Terzyk A. P., Wiśniewski M.

Przemysł Chemiczny

|

2016

|

T. 95, nr 11

2254--2258

PL

Przedstawiono problemy i ograniczenia stosowania immobilizowanych enzymów w procesach katalitycznych. Omówiono proces immobilizacji enzymów jako metodę poprawy ich stabilności w warunkach przemysłowych oraz wybrane techniki immobilizacji i nośniki enzymów.

EN

A review, with 42 refs., of polymeric enzyme carriers and nanocarriers (diamond, C tubes, graphene, graphene oxide) and practical uses of the immobilized enzymes.

2

Immobilizacja enzymów na nośnikach heterogenicznych. Cz. 1. Nośniki organiczne

Lasoń E., Ogonowski J.

LAB Laboratoria, Aparatura, Badania

|

2009

|

R. 14, nr 3/4

14-20

3

Immobilizacja enzymów. Część 2: Reaktory membranowe

Bryjdak J.

Wiadomości Chemiczne

|

2004

|

[Z] 58, 9-10

747--780

EN

Biocatalysis involves the enzyme-promoted transformations of a substrate into useful product in either a homogeneous or heterogenous system. The separation of reactants from products and the recovery and reuse of the catalyst from the reaction mixture is an important step that significantly decreases the cost of the process. Membrane reactors constitute an attempt to integrate catalytic conversion, product separation and/or concentration, and catalyst recovery into a single operation unit that lowers the overall cost of final product. In an enzyme membrane reactor biocatalyst is immobilized within the reaction vessel or on/in a membrane (catalytic membrane). The paper shows two possibilities for immobilization of enzymes: 1 - localization of the soluble biocatalyst in a certain defined region of space of a membrane reactor; e.g. a volume of a reactor and separation unit or in lumen/shell side of ultrafiltration unit; 2 - adsorbed, deposited or bound (physically, via affinity ligand or by covalent attachment) to the surface of a membrane or entrapped within pores or material of the membrane. Arguments counting for membrane supported or soluble enzymes are also presented. Reactors with soluble biocatalyst in a volume of a membrane reactor seem be suited to carry out complex enzymatic transformations, involving several enzymes and cofactor regeneration. Membrane reactors with catalytic membranes are particularly appropriate for nonconventional media such as organic-aqueous two-phase systems and for production of biosensing elements of enzyme electrodes. It is therefore the intention of the paper to outline the common immobilization methods and technologies to facilitate proper applications of enzymes immobilized in a membrane reactor.

4

Immobilizacja enzymów. Część 1: Metody konwencjonalne

Bryjdak J.

Wiadomości Chemiczne

|

2004

|

[Z] 58, 9-10

691--746

EN

Attractive features of biological systems include versatility, substrate selectivity, regio-, chemo-, and enantioselectivity as well as catalysis at ambient temperatures and pressure. However, the challenge facing bioprocesses is cost competitiveness with existing chemical process assets. The current expansion of industrial biocatalysis can be attributed to recent progress in molecular biology, advanced instrumentation, and engineering. Novel catalyst formulation based on technology such as directed evolution and enzyme immobilisation, has resulted in improved types of highly active and stable biocatalysts. In industrial biotransformations the immobilisation of enzymes on/in support is often chosen to enhance the stability and to simplify the biocatalyst recovery. The main purpose of this paper is to present a general picture of the immobilization techniques that is organised according to the two categories of methods, i.e., conventional methods (Part 1) and immobilisation in membrane reactors (Part 2). The intention of the first part is outline the common procedures that span from binding on carrier materials to incorporation into in situ prepared matrix in which binding forces vary between weak adsorption and covalent binding. Although the development of suitable immobilisation protocol often follows empirical guidelines, some general rules to facilitate proper applications are also presented. Immobilisation of enzymes means a deliberate restriction of the mobility of the enzyme, which can also affect mobility of the solutes. Thus it is mandatory to have basic knowledge of the essential contribution of the chemical forces and the physicochemical interactions during heterogeneous catalysis that is also discussed. Finally, the technological developments in the field of immobilised biocatalysts are presented that show possibility of a wide and more economical exploitation of enzymes in industry, medicine, and in the monitoring devices like the biosensors.