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Siderophore Production of the Hg-Resistant Endophytic Bacteria Isolated from Local Grass in the Hg-Contaminated Soil

Ustiatik Reni, Nuraini Yulia, Suharjono, Handayanto Eko

Journal of Ecological Engineering

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2021

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Vol. 22, nr 5

129--138

EN

Mercury (Hg)-contaminated soil remediation has become an urgent necessity due to its harmful effect on the environment and living organisms. The use of plant-endophyte partnership for phytoremediation demonstrates an excellent opportunity for cleaning heavy metal contaminated soil. This study aimed to screen and characterize the phenotype of the Hg-resistant endophytic bacteria from local grasses (Cynodon dactylon and Eleusine indica) in the Hg-contaminated soil of West Nusa Tenggara, Indonesia with siderophore-producing traits. Siderophore production of bacteria was qualitatively tested using overlay-chrome azurol S (O-CAS) medium and quantitatively tested using the succinic acid medium. The assay was designed using a Completely Randomized Factorial Design consisting of two factors, i.e., isolate type and incubation time with three replicates. The selected isolates were pathogenicity tested, then they were phenotypically characterized. All tested isolates showed a positive result on changing O-CAS medium color from blue to yellow/brown that indicated hydroxamate type of siderophore. The highest siderophore production was achieved at 72 hours of incubation, by the EI5 and EI6 isolates (62.90% and 35.31%, respectively). In turn, the CD6, EI5 and EI6 isolates achieved high siderophore production at a short incubation period (48 hours). However, during the hemolysis test, only the CD6 and EI6 isolates were not pathogenic. The CD6 and EI6 isolates would be used for phytoremediation on Hg-contaminated soil in the future study. On the basis of the 16S rDNA analysis, it was shown that the CD6 isolate was Jeotgalicoccus huakuii and the EI6 isolate was Bacillus amyloliquefaciens.

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Genomic Analysis of Plant-Associated Bacteria and Their Potential in Enhancing Phytoremediation Efficiency

Piński A., Hupert-Kocurek K.

Journal of Ecological Engineering

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2017

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Vol. 18, nr 4

152--159

EN

Phytoremediation is an emerging technology that uses plants in order to cleanup pollutants including xenobiotics and heavy metals from soil, water and air. Inoculation of plants with plant growth promoting endophytic and rhizospheric bacteria can enhance efficiency of phytoremediation. Genomic analysis of four plant-associated strains belonging to the Stenotrophomonas maltophilia species revealed the presence of genes encoding proteins involved in plant growth promotion, biocontrol of phytopathogens, biodegradation of xenobiotics, heavy metals resistance and plant-bacteria-environment interaction. The results of this analysis suggest great potential of bacteria belonging to Stenotrophomonas maltophilia species in enhancing phytoremediation efficiency.

3

Fitoremediacja terenów skażonych związkami ropopochodnymi z wykorzystaniem bakterii endofitycznych

Kukla M., Cania B., Płociniczak T., Piotrowska-Seget Z.

Przemysł Chemiczny

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2014

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T. 93, nr 3

355-359

PL

Ropa naftowa jest ważnym surowcem przemysłowym, ale jej wydobywanie, transport i przeróbka często prowadzi do skażenia środowiska naturalnego substancjami ropopochodnymi. Jedną z obiecujących biologicznych metod oczyszczania skażonych w ten sposób terenów jest fitoremediacja wspomagana przez bakterie endofityczne zasiedlające wnętrze roślin. Metoda ta jest skuteczna, ekonomiczna i przyjazna dla środowiska. Możliwość wykorzystania bakterii endofitycznych jest związana z ich właściwościami biochemicznymi (promowanie wzrostu roślin) i zdolnością do rozkładu węglowodorów. Jednak interakcje zachodzące pomiędzy bakteriami endofitycznymi a roślinami są wciąż stosunkowo słabo poznane. W celu pełnego wykorzystania ich potencjału do wspomagania fitoremediacji, niezbędne jest poszerzenie wiedzy na temat ich roli oraz wzajemnych oddziaływań między endofitami i roślinami.

EN

A review with 78 refs.