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PL
Od 1 listopada 2015 r. obowiązuje w Polsce Ustawa o leczeniu niepłodności. Celem artykułu jest przybliżenie podstawowych, wybranych regulacji prawnych dotyczących medycznie wspomaganej prokreacji.
EN
From November 1, 2015 comes into force in Polish the Act on the treatment of infertility. The aim of the article is to present the basic selected regulations on medically assisted procreation.
EN
The aim of the study was evaluation of possibility of poly-ε-caprolacton (PCL) application as potential material for production of medical devices, as catheters for obtaining and transporting of embryos as well as dishes for embryos culture in vitro and covers for cryoconservation. The possible application of this biomaterial needs verification of its biological properties on embryos culture. The foil discs made of policaprolacton, thickness 0.5 mm, diameter 3.5 mm, were prepared in two forms: the baseline one (nPCL) and thermally modified by freezing with liquid nitrogen (mPCL). The verification of PCL bioconcordance was performed by evaluation of 102 pig embryos. To evaluate poly-ε-caprolacton bioconcordance we performed 5-day long culture of embryos on the evaluated material, not frozen (nPCL) and frozen in liquid nitrogen (mPCL) and additionally culture after short contact with poly-ε-caprolacton, lasting 15 minutes (nPCL-15). In all evaluated study groups the development of embryos was suppressed shortly after transfer to the culture with PCL. In the control group. 74%-78% of embryos reached blastocyst stage. Polycaprolacton cannot be used as the material for catheter production used in biotechnology of animal reproduction and other materials used for in vitro culture and cryoconservation.
EN
Studies on the fecundity of V. viviparus were carried out in the years 1990-1994 in selected littoral sites of the Zegrzyński Reservoir (Central Poland) (60 km long, mean depth ca. 3.5 m, maximum depth at the dam up to 9 m, mean retention time from 1 to 15 days) and in outflow stretches of its tributaries. All sites were dominated by fertile females (i.e. having embryos) that lived in aggregations. Mean numbers of females ranged between 30 and 250 ind. m[^-2]. Females of III (shells 12.1 - 20.0 mm wide and 12.1 - 25.0 m high) and IV (20.1 - 25.0 mm width and 25.1 - 35.0 mm height) size class dominated. The embryos were noticed in females of II (8.1 - 12.0 mm width and height of the shell), III and IV size class and mean number of embryos per female was from 0.9 to 6.7, from 1.1 to 9.6 and from 2.0 to 9.1 for II, III and IV class respectively. Analysis of embryonic development revealed the presence of three growth stages in oviducts of females: the oval, transparent egg capsules called the youngest embryos), egg capsules with visible contour of a shell (called medium embryos) and snails with a shell (the oldest embryos). Embryos in a form of oval, transparent egg capsules and snails with developed shell dominated in particular size classes in analysed sites. Analysis of the correlation coefficient showed significant positive relationship between the mean sum of embryos and shell height and width, and dry weight of female's body and shell weight. Most significant correlation was found between dry body weight and mean number of embryos. The number of embryos in particular growth stages was also related to biometric indices as shown by the analysis of correlation coefficients. The number of oldest embryos (fully developed snails with a shell) increased with the increase of shell height and width. Female.s dry body weight, on the other hand, was best correlated with the youngest growth stage of embryos (oval, transparent egg capsules). Described relationships indicate the importance of the size of V. viviparus females for reproductive success and for survival and "condition" of young snails.
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