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Effect of cultivation factors on embryogenesis in isolated microspore culture of carrot (Daucus carota L.)

Voronina Anastasia V., Vishnyakova Anastasiia V., Monakhos Sokrat G., Monakhos Grigory F., Ushanov Alexander A., Mironov Aleksey A.

Journal of Water and Land Development

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2022

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no. 55

125--128

EN

Using doubled haploid technologies inbreeding can significantly reduce the time to obtain homozygous parental lines required for the production of F1-hybrid of vegetable crops. This study aims to investigate the influence of factors on the efficiency of carrot embryogenesis in isolated microspore culture to optimise the elements of protocol for producing doubled haploids. Microspores were isolated from inflorescences of 21 genotypes and incubated in NLN13 medium supplemented with 0.1 mg·dm-3 2,4-dichlorophenoxyacetic acids, 0.1 mg·dm-3 1-naphthyl acetic acids, 130 g·dm-3 sucrose, and 400 mg·dm-3 casein hydrolysate and its modifications. Embryoids and their groups were formed after 2–6 months, in some cases after 12 months of cultivation. Depending on the variant, the embryogenesis efficiency averaged from 0 to 4.9 embryoids or groups of embryoids per Petri dish (10 cm3). Embryoids within the group were formed from different microspores. No significant effects of inflorescence position on the plant (branching order), sucrose, and casein hydrolysate concentration in the medium were observed. Significant advantages (p ≥ 0.05) for some genotypes were shown: 1) microspore suspension density 4·104 cells·cm-3 (5.0 embryoids per Petri dish were formed at a microspore suspension density of 4·104 cells·cm-3, 0.0 embryoids per Petri dish at a density of 8·104 cells·cm-3); 2) cultivating microspores of tetrad and early mononuclear stage (4.9 ±3.1 embryoids per Petri dish were obtained by culturing tetrads and early mononuclear microspores, while 0.6 ±0.7 embryoids per Petri dish were obtained by culturing of later developmental stages); 3) high-temperature treatment duration of five days (4.9 ±2.1 embryoids per Petri dish were obtained after five days of high-temperature treatment, 2.7 ±2.6 embryoids per Petri dish formed after two days of high-temperature treatment; 9.8 ±4.7, 10.1 ±6.1, 0.0 ±0.0 embryoids per Petri dish formed after two, five and eight days of high-temperature treatment respectively); 4) adding colchicine 0.5 mg·dm-3 to the nutrient medium for two days of high-temperature treatment, followed by medium replacement (3.3 ±2.6 embryoids per Petri dish were obtained by using a nutrient medium with colchicine, while 1.7 ±1.5 embryoids per Petri dish were obtained by culturing in the reference variant).

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Configurational forces for growth and shape regulations in morphogenesis

Charletta P., Ambrosi D., Maugin G.

Bulletin of the Polish Academy of Sciences. Technical Sciences

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2012

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Vol. 60, nr 2

253-257

EN

Morphogenetic theories investigate the mechanisms of creation and regulation of definite biological forms in living organisms. The incredible diversity of shapes and sizes is generated through a barely unknown coordination of biochemical processes occurring at molecular levels. Such a crosstalk not only defines the rules of a robust scheme of matter differentiation, but it also has the capacity to adapt with respect to some variations of the environmental conditions. In this work, we propose a continuum model of growth and mass transport for biological materials during morphogenetic processes. Using the theory of configurational forces, we define the thermomechanical bases for understanding how both the mechanical and the biochemical states can orchestrate growth. The model is successfully applied to describe the morphogen-driven growth control in the imaginal wing disc of Drosophila melanogaster.

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Próba zastosowania termografii do monitorowania rozwoju termoregulacji zarodków kurzych (Gallus gallus)

Lis M. W., Augustyn J., Lisowska-Lis B., Niedziółka J. W.

Pomiary Automatyka Kontrola

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2011

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R. 57, nr 10

1150-1153

PL

Celem pracy było określenie przy pomocy pomiarów termowizyjnych tempa oddawania ciepła przez jajo kurze z rozwijającym się zarodkiem. Stwierdzono, że temperatura powierzchni skorupy jaja (EST) wzrasta w kolejnych etapach embriogenezy i może przewyższać temperaturę powietrza w inkubatorze. Niewielki spadek EST w 19. dobie inkubacji jaj umieszczonych w temperaturze pokojowej przez okres 15 minut, wskazuje na wykształcenie przez zarodek na tym etapie rozwoju zdolności ograniczonej termoregulacji.

EN

The aim of this study was to investigate the rate of heat transfer by a hen's egg with a developing embryo with use of infrared measurements. The study was conducted on 600 eggs for hatching chicken Ross 308 broiler line. The eggs were incubated in the setter S576 Petersime ® at the air temperature (T) equal to 37.8°C and relative humidity (RH) in the range 49-50%. Thermographic measurements of the eggshell surface temperature (EST) were taken with use of a thermal imaging camera ThermoVision A20 of a detector resolution 120x160 pixels. For statistical analysis of each egg there were used the EST highest values measured. Subsequent sessions were measured in the following days of incubation: E1, E3, E4, E7, E8, E9, E11, E12, E13, E16 and E19. Trays with eggs were removed from the incubator and placed at T = 22.4°C and RH = 75%, and recording of EST was performed: 1) immediately after removing the eggs from the incubator, 2) after 15 min, 3) after 30 min. It was found that the EST increased during the subsequent stages of embryogenesis. Before the start of the hatching process the temperature value may exceed the air temperature in the incubator by about 2 °C. The increase in the surface temperature of the egg shell is particularly intense from the 10th day of embryogenesis, reflecting the intensification of metabolism of the embryo. The slight decrease in the crust surface temperature of eggs placed at the room temperature for 15 minutes in the 19th day of incubation, compared to earlier stages of embryogenesis, shows that the embryo at this stage of development has the limited ability of thermoregulation.

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Morfomechaniczne zmiany jaj raka szlachetnego (Astacus astacus L.) w trakcie embriogenezy

Pawlos D., Korzelecka-Orkisz A., Wesołowski P., Formicki K.

Woda-Środowisko-Obszary Wiejskie

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2010

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T. 10, z. 3

201-214

PL

Stale wzrastające zanieczyszczanie środowiska oraz presja obcych gatunków raków, a przede wszystkim wyniszczenie przez dżumę raczą, spowodowało znaczne ograniczenie występowania stanowisk raka szlachetnego (Astacus astacus L.) w Polsce. Lepsze poznanie biologii rozrodu i embriogenezy tego gatunku raka jest konieczne nie tylko do jego ochrony przed wyginięciem, ale również poprawy warunków chowu i hodowli. Rozwój zarodkowy raka szlachetnego w warunkach średniej temperatury 8 ± 2°C trwał 2100 D°. Podział żółtka przebiegał w bardzo wolnym tempie i kończył się dopiero po upływie 352 D°. Pierwsze półkoliste zawiązki zarodka zaobserwowano w 592 D°. Zamykanie się blastoporu nastąpiło w 928 D°. W 1200 D° zarodek przybrał postać naupliusa. Na tym etapie widoczne były przydatki: anteny I i II pary oraz żuwaczki. Około 1500 D° serce podjęło pracę. Na krótko przed wylęgiem (1800 D°) pojawił się w oczach pigment, zaś na powierzchni zarodka zaobserwowano równomierne rozmieszczenie komórek barwnikowych. Po przeanalizowaniu zmian morfometrycznych jaj raka szlachetnego stwierdzono, że objętość w trakcie embriogenezy zwiększała się z 9,26 ± 0,44 mm³ do 13,5 ± 0,44 mm³ na krótko przed wylęgiem. Pomiary wytrzymałości mechanicznej osłonek jajowych ujawniły, że była ona dość znaczna na początku embriogenezy (ponad 150 ± 20,2 g) i zmniejszała się w trakcie rozwoju embrionalnego do wartości 83,5 ± 7,1 g w chwili wylęgu. Współczynnik S/V na początku rozwoju zarodkowego wynosił 2,291 i w miarę trwania embriogenezy zmniejszał się do 2,017.

EN

Constantly increasing pollution of the environment, invasive species, and a great harm caused by the plague (Aphanomyces astaci) contributed to a significant decrease of the noble crayfish (Astacus astacus) in Poland. Expanding the knowledge of the biology, reproduction and embryogenesis of the noble crayfish is necessary not only for its conservation, but also to improve its farming. Our study shows that embryogenesis of the noble crayfish at an average temperature of 8 ± 2°C lasted 2100 D°. The partition of the vitellus showed a rather slow rate and ended after 352 D°. First semicircular germs of the embryo were observed at 592 D°. Blastopore closed off at 928 D°. At 1200 D° embryo assumed the shape of nauplius, and at this stage the antennae of I, II pair and mandibles were visible. At about 1500 D° the heart became active. Shortly prior to hatching (1800 D°) eyes were pigmented, while steady distribution of pigment cell was observed on the surface of the embryo. After the analysis of morpho-mechanical changes in eggs of the noble crayfish, we found that the volume of eggs in the course of embryogenesis increased from 9.26 ± 0.44 mm³ to 13.5 ± 0.44 mm³ just shortly prior to hatching. Mechanical resistance of egg membrane to applied pressure was considerable - 150.0 ± 20.2 g at the beginning of embryogenesis, and dropped at hatching to 83.5 ± 7.1 g. The S/V coefficient at the beginning of embryonic development was 2.291 and decreased during embryogenesis to 2.017.

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Can photofrin accumulate in a rat embryo?

Legenis V., Zalgeviciene V., Tamosiunas M., Sukackaite A., Grazeliene G., Rotomskis R.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

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2004

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Vol. 10, nr 1-2

34-34