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HPLC analysis and blood-brain penetration of 20-hydroxyecdysone diacetonide

Kalász H., Hunyadi A., Tekes K., Dolesal R., Karvaly G.

Acta Chromatographica

2017

Vol. 29, no. 3

375--383

Blood-brain penetration of 20-hydroxyecdysone 2,3;20,22-diacetonide (20DA) has been scouted using chromatographic methods. In vivo experiments were performed by treating male Wistar rats intraperitoneally (i.p.) with a dose of 50 mg kg−1 20DA. Control experiment was done by using 20-hydroxyecdysone (20E). Definite brain penetration of 20DA was found by using high-performance liquid chromatography (HPLC), while 20E does not show this type of distribution.