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Ekstrakty z wybranych ziół jako bioaktywne źródło przeciwutleniaczy i inhibitorów enzymatycznych w aspekcie zastosowań w przemyśle

Skrzypek Alicja, Muszyński Paweł, Ciołek Anna

Przemysł Chemiczny

2025

T. 104, nr 11

1165--1170

Wysuszone próbki roślin z rodzin Lamiaceae, Apiaceae i Poaceae zmielono i ekstrahowano wodą lub 70-proc. etanolem poprzez wytrząsanie. Ekstrakty analizowano spektrofotometrycznie pod kątem całkowitej zawartości związków fenolowych (TPC) za pomocą odczynnika Folina i Ciocâlteu. Aktywność przeciwutleniającą ekstraktów określono na podstawie ich zdolności do neutralizacji rodnika DPPH, a ich potencjał hamujący wobec acetylocholinoesterazy (AChE) i butyrylocholinoesterazy (BChE) oceniono zmodyfikowaną metodą Ellmana. Obserwowano korelację pomiędzy zawartością TPC a aktywnością DPPH, natomiast nie stwierdzono korelacji między wysoką zawartością TPC a aktywnością antycholinoesterazową ekstraktów.

Dried plant samples from the Lamiaceae, Apiaceae, and Poaceae families were ground and extracted with water or 70% ethanol by shaking. The extracts were analyzed spectrophotometrically for total phenolic compound (TPC) content using the Folin-Ciocâlteu reagent. The antioxidant activity of the extracts was detd. based on their ability to neutralize the DPPH radical, and their inhibitory potential against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) was assessed using the modified Ellman method. A correlation was obsd. between TPC and DPPH activity, whereas no correlation was obsd. between high TPC content and anticholinesterase activity of the extracts.

The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

Zakłos-Szyda Małgorzata, Pawlik Nina

Acta Innovations

2019

no. 31

33--42

In recent years, research of antitumor activity of natural compounds isolated from plant material has increased. Polyphenols have gained significant attention due to their proapoptotic abilities and their involvement in migration and inhibition of metastasis processes. The anticancer effects of polyphenolic extracts of Viburnum opulus fruit against human breast (MCF-7) and cervical (HeLa) cancer cell lines have been confirmed in this study. It was demonstrated that the tested preparations (methanol – M and acetone – A from pomace, juice – J and juice after extraction to the solid phase SPE – PF) show cytotoxic activity and regulate the migration process of cancer cells. The degree of inhibition of cell migration was measured at two times - 24 h and 48 h after addition of the tested preparations. The highest toxicity towards both cell lines was demonstrated by the polyphenol fraction obtained after juice purification SPE (IC50 values at concentration of 63,541 and 19,380 μg/mL for HeLa and MCF cell lines, respectively). At the same time, the same preparation inhibited cell migration the most (nearly 70% compared to controls at both times at the concertation of 15 and 30 μg/mL). All preparations showed the antioxidant ability, but the Viburnum opulus juice (200 and 350 μg/mL) and the preparation after its purification (15 and 30 μg/mL) have larger ability to inhibit the intracellular oxidative stress (30-40%) than preparation obtained from pomace (nearly by 20% at concentration of 20 and 50 μg/mL of M and A). Despite the antioxidative capacity of the preparations, they simultaneously decreased cellular mitochondrial potential. The results obtained indicate the high potential of components of Viburnum opulus polyphenolic compounds can be used in the production of innovative dietary supplements or pharmacological preparations for people with an increased risk or inclination towards developing breast or cervical cancer.