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1 Materials Science Poland

1 2009

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Thin protein LB films as functional components within biosensors

Cabaj J., Chyla A., Sołoducho J.

Materials Science Poland

2009

Vol. 27, No. 3

684--692

Biomolecules can often be incorporated and immobilized into Langmuir-Blodgett (LB) films by using covalent immobilization of proteins with glutaraldehyde. Laccase from Cerrena Unicolor was incorporated into an LB film deposited on a glass substrate. The samples fabricated in such a way can be used for the detection of phenolic compounds. The function of enzyme immobilization was carried out by glutaraldehyde added to a film of stearic acid and laccase. The sensor sensitization was achieved by an amphiphilic N-alkyl-bis(thiophene)diphenylamine admixed into the film. The interlaced diphenylamine derivative was expected to facilitate the electron transfer thereby enhancing the sensor sensitivity.