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1 Materials Science Poland

1 2005

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Digital image analysis and visualization of early caries changes in human teeth

Kaczmarek E., Surdacka A., Matthews-Brzozowska T., Miśkowiak B.

Materials Science Poland

2005

Vol. 23, No. 2

551--558

The primary aims were the visualization, digital image analysis and X-ray EDS microanalysis to evaluate caries lesions in human premolars, with and without resin sealants, and light and scanning electron microscopic observations of hard dental tissues before and after the use of experimental protective strontium-fluoride toothpaste. The material consisted of healthy premolars extracted for orthodontic reasons, sectioned into slices. Sections of tooth crowns were digitized with a light microscope. The area of enamel demineralization was then measured. The in situ model was developed by placing the samples on the buccal surfaces of the first molars in healthy subjects, and exposing them to the use of the experimental toothpaste for 3 or 6 months. X-ray EDS microanalysis was undertaken to examine the Ca and Sr content on the enamel surface and at 15, 30, 60 and 100 microns depth. The results showed that the toothpaste induces an increased deposition of Ca in sub-superficial layers of the damaged enamel. The role of digital imaging needs to be defined with the diagnostic problem of the patient to plan an effective prophylaxis and treatment of early caries.