Wyniki wyszukiwania - BazTech

Ograniczanie wyników

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: daunorubicin

Sortuj według:

Ogranicz wyniki do:

The comparative study of influence of lactic and glycolic acids copolymers type on properties of daunorubicin loaded nanoparticles and drug release

Nikolskaya E., Sokol M., Faustova M., Zhunina O., Mollaev M., Yabbarov N., Tereshchenko O., Popov R., Severin E.

Acta of Bioengineering and Biomechanics

2018

Vol. 20, no. 1

65--77

The aim of this study was to compare the physico-chemical and biological properties of polymeric nanoparticles obtained from poly(DL-lactide-co-glycolide) (PLGA) with different ratios of monomers loaded with daunorubicin (DNR). Methods: DNR-loaded nanoparticles (NPs) were prepared with use of modified simultaneous double-emulsion solvent evaporation/diffusion technique. NPs were characterized using dynamic light scattering, atomic force microscopy, transmission electron microscopy, scanning electron microscopy, and differential scanning calorimetry and Fourier transform infrared spectroscopy. Results: NPs with DNR were differing in size and zeta potential, depending on the type of polymer. The data obtained show that total content of DNR correlates with the values of the binding constant of DNR with polymers. The release of DNR from NPs proceeds predominantly for polymers with lower binding constants. The in vitro study of NPs on the MCF-7 cells showed similar activity of particles and substances while for the anthracycline-resistant MCF-7Adr cells the cytotoxicity of the nanoparticles was 3 to 7 times higher depending on the type of copolymer. Conclusions: PLGA DNR-loaded nanoparticles can be used to overcome multidrug resistance (MDR) as well as for reducing the frequency of DNR reception due to the prolonged effect, which allows maintaining the concentration of the drug at the required level. The usefulness of binding constant calculations for obtaining nanoparticles with the maximum drug loading was proven. The rate of drug administration and the frequency of administration can be calculated based on the DNR release profiles and release parameters that depend on polymer type.

Development of validation of RP-HPLC method for determination of novel derivatives of daunorubicin

Łukawska M., Oszczapowicz I., Jelińska A., Cielecka-Piontek J., Zając M., Dobrowolski L., Ziółkowska G., Zalewski P., Piekarski M., Krause A., Uszak J.

Chemia Analityczna

2009

Vol. 54, No. 5

907-917

An isocratic HPLC method with UV detection for simultaneous determination of four novel derivatives of daunorubicin and determination of selected derivatives in commonly used intravenous solvents was developed and validated. An RP-column and an acetonitrile-metha-nol-(2.88 g L-1 sodium laurisulfate + 1.6 mL L-1 85% H3PO3), 9:1:10 mobile phase at a flow rate of 1.5 mL min-1 were used. Detection wavelength was 254 nm. The method was validated with regard to selectivity, linearity, precision, limit of detection, limit of quantitation, and robustness, and did not require any preliminary treatment of samples. It can be used for identification and determination of derivatives of daunorubicin in phar-maceuticals and during kinetic studies of these derivatives.

Opracowano i zwalidowano izokratyczną metodę HPLC z detekcją UV do oznaczania czterech nowych pochodnych daunorubicyny oraz oznaczania wybranych pochodnych w powszechnie stosowanych płynach infuzyjnych. Zastosowano kolumnę RP i fazę ruchomą acetonitryl-metanol-roztwór zawierający 2,88 g L-1 laurylosiarczanu sodu i 1,6 ml L-1 H4PO4, o szybkości przepływu l ,5 mL min-1. Detekcję przeprowadzono przy długości fali detektora 254 nm. Metoda została zwalidowana pod względem selektywności, liniowości, precyzji, wykrywalności, oznaczalności i odporności na zmiany; nie wymagała wcześniejszej derywatyzacji próbek. Opracowana metoda HPLC może być zastosowana do jednoczesnego lub osobnego oznaczania pochodnych daunorubicyny w preparatach farmaceutycznych i podczas badań kinetycznych powyższych pochodnych.