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Detection of two major cytochrome b lineages in pike-perch, Sander lucioperca, and first data on their distribution in European populations

Kohlmann K., Louati M., Kersten P., Bahri-Sfar L., Poulet N., Ben Hassine O. K.

Environmental Biotechnology

2013

Vol. 9, No. 1

1--5

Despite of the growing interest in pike-perch for aquaculture and its economic importance in fisheries, knowledge on the population structure and phylogeography of the species is still limited. We report the discovery of two major cytochrome b lineages and describe a simple method for their detection based on PCR amplification followed by restriction digestion with Alw26I. Screening of 708 individuals showed that haplotype A was fixed or dominating in Central and East European countries, whereas haplotype B was mainly found in several French populations and Tunisian pike-perch introduced from Europe. Sequencing the complete cytochrome b cds of 17 representative individuals revealed that haplotypes A and B differed by five substitutions but also showed further differentiation of both haplotypes due to an additional substitution in a single haplotype A and B individual from France, respectively. Five partial pike-perch cytochrome b cds available from NCBI GenBank could also clearly be assigned to one or the other of the two major lineages. Therefore, this new mtDNA marker might be considered as suitable not only for studies on population structure and phylogeography of pikeperch but also to trace its introduction history and to assess the genetic composition of aquaculture brood stocks.

Phylogenetic discontinuity of plateau zokor (myospalax baileyi thomas) populations in qinghai-tibetan plateau

Tang L., Zhang T., Lin G., Su J.

Polish Journal of Ecology

2010

Vol. 58, nr 1

167-176

Mitochondrial cytochrome b gene sequences and morphological data (body length, hind foot length, etc.) for twelve populations with pairwise distances 27-600 km in Qinghai-Tibetan Plateau (distributed in Qinghai, Gansu and Sichuan Province, and at the altitude 3020-4550 m) in Western China were used to investigate the phylogeographical pattern of Plateau zokor (Myospalax baileyi Thomas). There was a little disparity between mtDNA genetic distance and morphological Euclidean distance on population relationships. However, there is a significant correlation (P <0.001) calculated by Mantel's tests was validated between mtDNA and morphology distances. Analysis of Molecular Variance showed that most of the observed genetic variations occurred between populations, indicating little maternal gene flow between them, as a result of geographical restrictions. Phylogenetic analysis coupled with cluster analysis together showed that the substantial population structuring and phylogenetic discontinuities existed within this species. The evident allopatric population structuring of this subterranean rodent may mostly result from its specialized subterranean excavating behavior with high energy costs, predation from grassland raptors and also the influences of perennial tundra and environmental desiccation in the Qinghai-Tibetan Plateau.