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Evaluation of Candida albicans adhesion on AlSl-316L and Ti6Al4V in changing environmental conditions

Jastrzębska A., Jakubowski W., Jastrzębski K., Walkowiak B.

Engineering of Biomaterials

2017

Vol. 20, no. 141

2--7

Currently, studies on the formation of microbiological biofilm on biomaterial surfaces and medical devices have become an important aspect in medicine. In vivo studies of the biofilm development show a high correlation between the environmental factors and attachment of microorganisms to artificial surfaces. This paper is devoted to determining the impact of specific environmental stimuli and parameters of cultivation of Candida albicans yeasts on their adhesion to AISI-316L and Ti6Al4V substrates. Environmental parameters include variable glucose concentrations in YPG growth medium (0, 2, 4 and 10% wt.). Development of yeasts was also analyzed in terms of variable nutrients level i.e. transferring samples from medium with low nutrients level to other with higher glucose content. Another studied parameter was incubation time and how much fungal colonization varies during 24 and 48 h of incubation. For all samples, the number of both live and dead cells was taken into account. The results showed that with the increase in sugar content in culture media, biofilm development was observed, especially on AISI 316L surface. Also, significant changes in the number of adhered cells were observed with higher glucose concentration in medium after 24 h of incubation. Almost similar number of attached cells was observed for low glucose concentrations on both studied materials.

Effect of Gluconacetobacter xylinus cultivation conditions on the selected properties of bacterial cellulose

Fijałkowski K., Żywicka A., Drozd R., Kordas M., Rakoczy R.

Polish Journal of Chemical Technology

2016

Vol. 18, nr 4

117--123

The aim of the study was to analyze the changes in the parameters of bacterial cultures and bacterial cellulose (BC) synthesized by four reference strains of Gluconacetobacter xylinus during 31-day cultivation in stationary conditions. The study showed that the most visible changes in the analyzed parameters of BC, regardless of the bacterial strain used for their synthesis, were observed in the first 10–14 days of the experiment. It was also revealed, that among parameters showing dependence associated with the particular bacterial strain were the rate and period of BC synthesis, the growth rate of bacteria anchored to the cellulose fibrils, the capacity to absorb water and the water release rate. The results presented in this work may be useful in the selection of optimum culturing conditions and period from the point of view of good efficiency of the cellulose synthesis process.