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Densitometric HPTLC analysis of cordifolioside A in Tinospora cordifolia and commercial formulations

Alam P., Ali M., Singh R., Shakeel F.

Acta Chromatographica

2009

Vol. 21, no. 4

683--692

A densitometric HPTLC method for analysis of cordifolioside A both in 60% methanolic extract of Tinospora cordifolia and in a commercial formulation has been established and validated. Cordifolioside A was separated on aluminum-backed silica gel 60 F254 plates with chloroform-methanol 85:15 (%, v/v ) as mobile phase. A compact band was obtained for cordifolioside A at R F 0.52 ± 0.03. The limits of detection (LOD) and quantification (LOQ) were 20.12 and 60.36 ng per band, respectively. The highly precise and accurate method was used for analysis of cordifolioside A.