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Olive oil mill waste is characterized by its high organic matter content, especially fatty acids, polyphenols, sugars, and proteins. These nutrients can be used as a source of energy for biogas production. However, olive oil mill waste can also contain heavy metals such as lead, cadmium, copper, and zinc that can be absorbed by plants. In addition, very high concentrations of heavy metals can also inhibit the anaerobic digestion process by affecting the methanogenic bacteria involved in biogas production The aim of this research is to determine the composition of solid and liquid rejections from traditional and continuous three-phase crushing systems, by analyzing samples from different oil mills in the eastern region of Morocco. We also applied the technology of anaerobic digestion of solid and liquid waste forms of oil mills, to make a link between the biogas yield and the physicochemical characteristics of these wastes. The results suggest that traditional oil mill wastewater (Discontinuous OMWW) has high organic matter, nutrients, and heavy metals content and a low concentration of phenolic compounds, which can increase its biogas production potential with a production of 10.02 Nml/g VS, while three-phase wastewater (Continuous OMWW) has limited biogas production potential (3.83 Nml/g VS) due to the low organic matter and nutrients content, and high concentration of phenolic compounds. Three-phase olive pomace (Continuous OMSW) has a higher biogas production (9.28 Nml/ g VS) than traditional olive pomace (Discontinuous OMSW) with 5.91 Nml/g VS. In fact, the lower content of phenolic compounds and volatile fatty acids favors their anaerobic digestion and improves their biogas production. In conclusion, the selection of the type of waste adapted for biogas production must be based on the physicochemical and microbiological characteristics of these wastes.
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