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Point spread function in a confocal microscope with trigonometric pupil filters.

Magiera A.

Optica Applicata

2000

Vol. 30, nr 2-3

455-461

The distribution of point spread function was examined versus the spatial frequencies of the filters of cos(Np) type modulating the aperture of the confocal CSM for different values of numerical aperture. In particular, the following relations were determined: (i) PSF as dependent on the pupil modulating spatial frequency r for N=1, 2, 3, 4, 5, 6, 7, 9, 11 and numerical aperture NA=0.8; (ii) PSF for filters of cos(4 rho ), cos(10 rho ) type and NA=0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4; (iii) cut-off spatial frequencies r/sub c/ for the aperture modulated by cos(N rho ) for N=0,..., 20, NA=0.2, 0.5, 1.0 and lambda =0.6328 mu m.