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Vascular smooth muscle cells in cultures on low density polyethylene modified with plasma discharge and biofunctionalization

Parizek M., Kasalkova N., Bacakova L., Kolarova K., Lisa V., Svorcik V.

Engineering of Biomaterials

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2009

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Vol. 12, no. 89-91

25--28

EN

Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.

2

The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films

Parizek M., Base T., Londesborough M. G. S., Lisa V., Bacakova L.

Engineering of Biomaterials

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2008

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Vol. 11, no. 81-84

117--119

EN

Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2B10H11 (A), 1,2-(HS)2-1,2-C2B10H10 (B), 9,12-(HS)2-1,2-C2B10H10 (C) and 1,12-(HS)2-1,12- C2B10H10 (D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.

3

The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films

Parizek M., Base T., Londesborough M. G. S., Lisa V., Bacakova L.

Engineering of Biomaterials

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2008

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Vol. 11, no. 75

6--8

EN

Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2 B10H11(A), 1,2-(HS)2-1,2-C2B10H10(B), 9,12-(HS)2-1,2-C2B10H10(C) and 1,12-(HS)2-1,12- C2B10H10(D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.

4

Improved adhesion and growth of vascular smooth muscle cells in cultures on polyethylene modified by plasma discharge

Parizek M., Kasalkova N., Bacakova L., Kolarova K., Lisa V., Svorcik V.

Engineering of Biomaterials

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2007

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Vol. 10, no. 67-68

1-4

EN

The attractiveness of synthetic polymers for cell colonization can be affected by physical and chemical modification of the polymer surface. In this study, high density polyethylene (HDPE, m.w. 0.952g/cm3) and low density polyethylene (LDPE, m.w. 0.922g/cm3) were modified by an Ar plasma discharge using Balzers SCD 050 device (exposure time 10, 50, 150 and 400 seconds, discharge power 1.7W). The material was then seeded with rat aortic smooth muscle cells (RASMC; passages 8 to 9, 17 000 cells/cm3) and incubated in a DMEM medium with 10% of fetal calf serum. On day 1 after seeding, the number of initially adhered cells was significantly higher on all modified HDPE and LDPE samples. On day 2, this difference persisted in HDPE, whereas in LDPE only the values on the samples modified by 150 and 400 seconds were significantly higher. On the 5th and 7th day, there were no significant differences in cell number among all LDPE samples. However, on the HDPE foils, significant differences were still apparent on the samples modified for 400 seconds. The cell spreading areas measured on day 1 after seeding were significantly larger on all modified LDPE samples, and, on day 2, on the HDPE samples exposed for 150s. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups in the polymer. These results suggest that the responsiveness of the cell to the changes in physiochemical surface properties was more pronounced in HDPE than in LDPE. On both types of polyethylene, the most appropriate exposure time for the enhancement of cell adhesion and growth seemed to be 150 and 400 seconds.

5

Adhesion and growth of human osteoblast-like cells on aliphatic polyesters with different chemical composition, surface roughness and modification with hydroxyapatite

Vagaska B., Bacakova L., Pamuła E., Lisa V., Dobrzyński P.

Inżynieria Biomateriałów

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2006

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R. 9, nr 58-60

4-7

EN

In this study we have investigated the effect of three groups of polymeric foils on the behavior of MG 63 osteoblast-like cells. These included (1) poly(L-lactide) (PLLA) compared with newly synthesized copolymer of L-lactide and trimethylene carbonate (PLTMC 50:50), (2) three samples made of glycolide and epsylon-caprolactone copolymer (PGCap) with different surface roughness and topography, and finally (3) copolymer of glycolide with L-lactide (PGLA) compared with its modification with hyroxyapatite deposits. On the 1st and 4th day of cultivation the cell number on all of the samples was lower than on control polystyrene culture dish. However, on day 8 after seeding, the values on the tested samples caught up with the control polystyrene. In the first group the cell number of PLTMC was higher than on polystyrene or PLLA. In the second group, the number of cells on PGCap samples of the lower surface roughness (RRMS 130 and 180 nm) was significantly higher than that on the control polystyrene, whereas on the PGCap samples with the rounghness in micrometers, it was comparable to the value on the polystyrene. Moreover, the surface roughness influenced the cell adhesion area. The cells on the sample with the highest roughness index were roundly shaped and their adhesion area was significantly lower, because the cells were restricted in their spreading by the surface structure of the material. In the last group, the number of cells on day 8 on the polymer with hydroxyapatite deposits was significantly higher than on standard tissue culture polystyrene dish, as well as on unmodified PGLA foil, which suggested that hydroxyapatite supports cell proliferation.

6

The combination of the overpressured layer chromatography and bioautography and its applications to the analysis of molecules influencing cell proliferation

Tyihák E., Botz L., Ott P., Nagy S., Kocsis B., Király-Véghely Z., Mincsovics E.

Chemia Analityczna

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2003

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Vol. 48, No. 3

543-553

EN

BioArena is a complex separation and detection system, which integrates the advantages of the overpressured layer chromatography (OPLC) and bioautography. It exploits the interaction possibilities between the microbes and the dye substance as well as other small and big co-factor molecules in the sorbent bed after separation. OPLC separation resulted in the increase of the sensitivity and the compactness of the spots in comparison to the conventional TLC-HPTLC separations. OPLC and bioautography as a detection system facilitate studying different biological interactions in the field of antibiosis, especially model reactions of endogenous formaldehyde (HCHO) and other small endogenous molecules (e.g. H(2)O(2), as well as /rans-resveratrol.

PL

BioArena jest złożonym układem, który łączy w sobie korzyści nadciśnieniowej chromatografii warstwowej (OPLC) i bioautografii. Wykorzystuje ona możliwości oddziaływania drobnoustrojów z substancją barwiącą, jak również z innymi małymi i dużymi cząsteczkami jako czynnikami dopełniającymi, w złożu sorbentu po rozdzieleniu. Zastosowanie OPLC zaowocowało wzrostem czułości oraz polepszeniem zwartości plamek w porównaniu do konwencjonalnych rozdzielań za pomocą TLC-HPTLC. OPLC i bioautografia jako układ detekcyjny stwarzają korzystne możliwości badania różnych oddziaływań biologicznych w zakresie antybiozy ze zwróceniem szczególnej uwagi na modelowanie reakcji endogennego formaldehydu (HCHO) i innych małych endogennych cząsteczek (np. H(2)O(2) jak również fra/Łs-resveratrolu.