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Image processing algorithm for cell force sensor with a micropillar patterned substrate

Smolik W. T., Święszkowski W., Kurzydłowski K. J., Bruinink A., Danz N.

Biocybernetics and Biomedical Engineering

2010

Vol. 30, no. 3

49-64

The paper presents an image analysis for cell force measurement using a patterned substrate surface. The cell forces are transduced to a hexagonal lattice of microcantilevers in the shape of cylindrical pillars installed on a substrate surface. The magnitude and direction of pillars deflection is measured optically by an analysis of transmission images of the substrate. The template matching is used for identification of the position of pillars. The hexagonal lattice of pillars is identified automatically and used as information about the reference rest position of the pillars. The elaborated algorithm was tested using simulated data. The first results using real images are presented.

Nanomechanics = biomechanics

Skubiszak L.

Bulletin of the Polish Academy of Sciences. Technical Sciences

2009

Vol. 57, nr 1

47-53

The knowledge of the mechanism of mechanical energy production by the so-called bioengines, living cells, could be very helpful for resolving different tasks concerning nanomechanics, e.g., construction of nanorobots. The present work considers a new idea, namely that the conformational changes within the so-called track, actin filament or microtubule are crucial for production of the mechanical energy by all bioengines. This concept contrasts with the presently prevailing view, according to which the force is generated as a result of conformational changes within the so-called motor proteins: myosin, kinesin or dynein.