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Effect of topography parameters on cellular morphology during guided cell migration on a graded micropillar Surface

Krishnamoorthy Srikumar, Xu Heqi, Zhang Zhengyi, Xu Changxue

Acta of Bioengineering and Biomechanics

2021

Vol. 23, no. 2

147--157

Purpose: Guided cell migration refers to the engineering of local cell environment to specifically direct cell migration and has important applications such as utilization in cell sorting and wound healing assays. Graded micropillar surfaces have been utilized for achieving guided cell migration. Topographic parameters such as micropillar diameter and spacing gradient may have effects on the morphology of attached cells. It is critical to understand this interaction between the cells and the underlying microscale structures. Methods: In this study, a graded micropillar substrate has been fabricated to investigate the effects of the microtopography on the cell morphology in terms of the cell aspect ratio and cell circularity. Results: It is found that 1) with the increase of the micropillar diameter, the cell aspect ratio has no significance change. At the small spacing gradients, the aspect ratio is smaller than that at the large spacing gradients; 2) statistical analysis shows both the micropillar diameter and spacing gradient have no significant effect on the cell aspect ratio compared to the flat surface; 3) the cell circularity at the small micropillar diameters is higher than that at the large micropillar diameters. The cell circularity at the micropillar gradient of 0.1 µm is higher than those at the other micropillar gradients; 4) three microtopographic conditions are considered to have statistically significant effect on the cell circularity compared to the flat surface, including the micropillar diameters of 5 µm and 10 µm and the spacing gradient of 0.1 µm.

Microscopic analysis of the nanostructures impact on endothelial cells

Kołodziejczyk Agnieszka Maria, Kucińska Magdalena, Jakubowska Aleksandra, Siatkowska Małgorzata, Sokołowska Paulina, Kotarba Sylwia, Makowski Krzysztof, Komorowski Piotr, Walkowiak Bogdan

Engineering of Biomaterials

2020

Vol. 23, no. 154

2--8

Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.

Influence of nanocrystalline structure and surface properties of TiO2 thin films on the viability of L929 cells

Osękowska M., Karuga-Kuźniewska E., Wojcieszak D., Mazur M., Poniedziałek A., Kaczmarek D., Szymonowicz M., Rybak Z.

Polish Journal of Chemical Technology

2015

Vol. 17, nr 3

33--39

In this work the physicochemical and biological properties of nanocrystalline TiO2 thin films were investigated. Thin films were prepared by magnetron sputtering method. Their properties were examined by X-ray diffraction, photoelectron spectroscopy, atomic force microscopy, optical transmission method and optical profiler. Moreover, surface wettability and scratch resistance were determined. It was found that as-deposited coatings were nanocrystalline and had TiO2-anatase structure, built from crystallites in size of 24 nm. The surface of the films was homogenous, composed of closely packed grains and hydrophilic. Due to nanocrystalline structure thin films exhibited good scratch resistance. The results were correlated to the biological activity (in vitro) of thin films. Morphological changes of mouse fibroblasts (L929 cell line) after contact with the surface of TiO2 films were evaluated with the use of a contrast-phase microscope, while their viability was tested by MTT colorimetric assay. The viability of cell line upon contact with the surface of nanocrystalline TiO2 film was comparable to the control sample. L929 cells had homogenous cytoplasm and were forming a confluent monofilm, while lysis and inhibition of cell growth was not observed. Moreover, the viability in contact with surface of examined films was high. This confirms non-cytotoxic effect of TiO2 film surface on mouse fibroblasts.