Wyniki wyszukiwania - BazTech

1

Osteoblasts response to novel chitosan/agarose/hydroxyapatite bone scaffold – studies on MC3T3-E1 and hFOB 1.19 cellular models

Kazimierczak Paulina, Vivcharenko Vladyslav, Truszkiewicz Wiesław, Wójcik Michał, Przekora Agata

Engineering of Biomaterials

|

2019

|

Vol. 22, no. 151

24--29

EN

Since it is known that various cell lines may ex-press different behaviours on the scaffolds surface, a comprehensive analysis using various cellular mo-dels is needed to evaluate the biomedical potential of developed biomaterials under in vitro conditions. Thus, the aim of this work was to fabricate bone scaffolds composed of a chitosan-agarose matrix reinforced with nanohydroxyapatite and compare the biological response of two cell lines, i.e. mouse calvarial preosteoblasts (MC3T3-E1 Subclone 4) and human foetal osteoblasts (hFOB 1.19). Within this study, the osteoblasts number on the scaffold surface and the osteogenic markers level produced by MC3T3-E1 and hFOB 1.19 cells were determined. Furthermore, changes in calcium and phosphorous ions concentrations in the culture media dedicated for MC3T3-E1 and hFOB 1.19 were estimated after the biomaterial incubation. The obtained results proved that the fabricated biomaterial is characterized by biocompatibility and osteoconductivity since it favours osteoblasts attachment and growth. It also supports the production of osteogenic markers (collagen, bALP, osteocalcin) by MC3T3-E1 and hFOB 1.19 cells. Interestingly, the developed biomaterial exhibits different ion reactivity values in the two culture media dedicated for the mentioned cell lines. It was also revealed that mouse and human osteoblasts differ in the cellular response to the fabricated scaffold. Thus, the use of at least two various cellular models is recommended to carry out a reliable biological characterization of the novel biomaterial. These results demonstrate that the tested bone scaffold is a promising biomaterial for bone regeneration applications, however further biological and physicochemical experiments are essential to fully assess its biomedical potential.

2

Black Orlon as promising material for bone tissue engineering

Parizek M., Vetrik M., Hruby M., Lisa V., Bacakova L.

Engineering of Biomaterials

|

2014

|

Vol. 17, no. 128-129

4--6

3

Improved adhesion and growth of osteoblast-like MG-63 cells in cultures on titanium modified by gold particles

Parizek M., Base T., Hruby M., Lisa V., Bacakova L.

Engineering of Biomaterials

|

2013

|

Vol. 16, no. 122-123 spec. iss.

77

EN

Metallic materials are important for load-bearing bone implants. The osteointegration of these implants can be improved by appropriate surface modifications. Therefore, we present here a study of the cell growth on titanium surfaces modified with films created from gold microparticles. These particles in the form of microplates or polyhedral microcrystals were deposited on titanium plates from ethanol solutions, dried and annealed with a hydrogen flame. Some samples were additionally modified by polyethylene imine. The materials engendered from these modifications were used to investigate the adhesion and growth of human osteoblast-like MG-63 cells on these surfaces in the DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surfaces modified by both types of gold microparticles. This trend was the same three and seven days after seeding. The numbers of cells on pure Ti and Ti modified only with gold particles were significantly higher than on samples which were modified with polyethylene imine. The cell spreading areas projected on the materials were significantly larger in cells on the samples with polyethylene imine modification. However, the shape of these cells was mostly rounded or star-like with thin and long protrusions, while on the materials without polyethylene imine, it was mostly polygonal. The cell proliferation activity was estimated from XTT test, based on the activity of mitochondrial enzymes. This test showed that the proliferation activities of osteoblast-like MG-63 cells of the 3rd and 7th days of the experiment were more pronounced on the samples modified only by gold microparticles. Immunofluorescence showed that the focal adhesion plaques containing vinculin and the fibers containing β-actin were most apparent, more numerous and more brightly stained in cells on Ti modified by gold microplates and gold polyhedral microcrystals, especially in comparison with the corresponding samples modified with polyethylene imine (Fig. 1). Thus, it can be concluded that the modification of titanium samples by both types of gold microparticles enhanced the adhesion and growth of MG 63 cells.

4

Surface characterization, collagen adsorption and cell behaviour on poly(L-lactide-co-glycolide)

Adamczak M., Ścisłowska-Czarnecka A., Genet M. J., Dupont-Gillain C. C., Pamula E.

Acta of Bioengineering and Biomechanics

|

2011

|

Vol. 13, no. 3

63-75

EN

Poly(L-lactide-co-glycolide) (PLG) was modified through the adsorption of collagen to improve the behaviour of fibroblasts and osteoblasts. As reference materials cell-resistant polystyrene (PS) and cell-conductive tissue-culture polystyrene (TCPS) were also evaluated. The physicochemical surface properties of the materials were studied by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle measurements. The morphology of cells was examined using optical microscopy, while their growth was evaluated by both crystal violet and MTT tests. Nitric oxide level and protein concentration were tested in cell supernatants. The results showed that the adsorbed amount and the organization of the adsorbed collagen were influenced by surface hydrophobicity. Cell culture experiments on native substrates revealed that cell attachment, spreading and growth enhanced, depending on the substrate, in the following order: PS