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Spektroskopia MRS w hodowlach komórkowych in vitro

Truszkiewicz Adrian, Aebisher David, Bartusik-Aebisher Dorota

Inżynier i Fizyk Medyczny

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2020

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Vol. 9, nr 3

175--178

PL

Magnetyczny Rezonans Spektroskopowy (MRS) mimo swojej siedemdziesięcioletniej historii jest dziedziną ulegającą stałemu i niesłabnącemu rozwojowi. Wiąże się to z możliwością wszechstronnego wykorzystania pomiarów MRS zarówno eksperymentalnie do badań podstawowych z zakresu biochemii i fizyki (wyznaczania pewnych parametrów kinetycznych i termodynamicznych), jak i klinicznie w praktyce medycznej. Jako obiekt badań wybrane zostały hodowle komórkowe, a wyniki badania spektroskopowego w hodowlach komórek nowotworowych są prezentowane w niniejszym opracowaniu. Celem pracy była optymalizacja pomiaru spektroskopowego w hodowlach komórkowych na średniopolowym systemie magnetycznego rezonansu jądrowego. Zapotrzebowanie na jakościowe i ilościowe analizy zawartości aminokwasów w mediach i różnych płynach fizjologicznych i poznanie składu mieszanin aminokwasów białek spowodowało rozwój wielu technik spektroskopowych, m.in. MRS.

EN

Magnetic Resonance Spectroscopy (MRS), despite its seventy years of history, is a field undergoing constant and uninterrupted development. This involves the possibility of comprehensive use of MRS measurements both experimentally for basic research in the field of biochemistry and physics (determination of certain kinetic and thermodynamic parameters) as well as clinically in medical practice. Cell cultures were chosen as the object of research and the results of spectroscopic examination in cancer cell cultures are presented in this study. The aim of the work was to optimize spectroscopic measurement in cell cultures on a medium-field nuclear magnetic resonance system. The need for qualitative and quantitative analysis of the content of amino acids in the media and various physiological fluids and knowledge of the composition of amino acid mixtures of proteins has resulted in the development of many spectroscopic techniques, among others MRS.

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Ocena przydatności średniopolowych systemów MR w obrazowaniu hodowli komórkowych

Truszkiewicz Adrian, Bartusik-Aebisher Dorota

Inżynier i Fizyk Medyczny

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2020

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Vol. 9, nr 1

37--40

PL

W niniejszym artykule autorzy podjęli próbę oceny przydatności systemów rezonansu magnetycznego o sile 1,5 Tesla do obrazowania małych struktur, jakimi są hodowle komórkowe. Zakres obrazowania, jaki stał się przedmiotem badań, wkracza w obszar będący domeną mikroskopii rezonansu magnetycznego (MRM). W dzisiejszej dobie badania te wykonywane są przy użyciu systemów wysokopolowych zapewniających dużo większe rozdzielczości przestrzenne. Celem pracy było określenie warunków, jak również parametrów skanowania pozwalających na obrazowanie małych obiektów przy wykorzystaniu systemu OPTIMA MR360 produkcji GE Healthcare. W wyniku prac koniecznym stało się opracowanie dodatkowej cewki solenoidalnej, której to zarówno wymiary, kształt, jak i konstrukcja zapewniły optymalną jakość obrazowania możliwą do uzyskania w oparciu o w/w system MR. Artykuł ten jest pierwszym z serii prac ukazujących badania hodowli komórkowych z użyciem rezonansu magnetycznego w Uniwersytecie Rzeszowskim.

EN

In this article, the authors attempt to assess the usefulness of 1,5 Tesla magnetic resonance imaging systems for imaging of small structures such as cell cultures. The scope of imaging that has become the subject of research enters the area that is the domain of magnetic resonance microscopy (MRM). Nowadays, these tests are performed using high-field systems providing much higher spatial resolutions. The purpose of the work was to determine the conditions as well as scanning parameters that allow imaging of small objects using the OPTIMA MR360 production system. GE Healthcare. As a result of the work, it became necessary to develop an additional solenoid coil, which both dimensions, shape and design ensured optimal imaging quality possible based on the above MR system. This article is the first in a series of works showing the study of cell cultures using magnetic resonance imaging at the University of Rzeszów.

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Systemy Cell-on-a-chip jako zaawansowane modele hodowli komórek in vitro

Jastrzębska Elżbieta

Wiadomości Chemiczne

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2019

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[Z] 73, 11-12

715--735

EN

In vitro study at the cellular level is an important step in evaluation of biological properties of newly developed drugs. In addition, thanks to the use of cell cultures, it is possible to study the function of the cells of a specific tissue and to mimic the conditions of cell growth or disease state. Standard (conventional) cell cultures are most often based on monolayer (two-dimensional, 2D) culture. In such culture, the cells adhere to the surface of the bottle/plate in which they grow. Nevertheless, spatial (three-dimensional, 3D) cultures are also increasingly used in culture research. However, conventional cell cultures still have a limited ability to mimic the natural environment of cell growth. Lab-on-a-chip systems (called Cell-on-a-chip) are new miniature constructional and methodical solutions useful for in vitro cell cultures. Microsystems allow the development of more advanced cell models than standard in vitro cultures so far used in biological laboratories. Thanks to this, it is possible to study cell functions under conditions that mimic the natural environment of cell growth. It should be emphasized, that the microsystems cannot completely replace animal testing. Cell-on-a-chip systems can provide alternative or complementary solutions / tools for in vivo tests, while ensuring high reliability of the obtained results. So far, Lab-on-a-chip systems have been used to assess the cytotoxicity of compounds, model intercellular interactions and mimic the function of various tissues. In this article we describe Cell-on-a-chip systems which can be used as advanced models of cell cultures. At the beginning of the manuscript, Cell-on-a- chip systems and materials used for fabrication microsystems are characterized. Next, the microsystems used as platforms for cytotoxicity study are presented. A special attention has been paid to Cell-on-a-chip for spatial cell cultures using: spheroids, hydrogels, multilayers and nanoscaffolds. Finally, Organ-on-a-chip systems are shortly discussed. Schemes of Cell-on-a-chip system, types of cell cultures obtained in microscale are presented in the article. A table showing a construction materials is also added. At the end, the article is summarized.

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Biological effects of the EMF influence on animal cells and tissues in in vitro cultures – a summary of own research

Koziorowska A.

Przegląd Elektrotechniczny

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2018

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R. 94, nr 12

206--209

EN

The effects of the electromagnetic field on cells and tissue in in vitro cultures are the subject of the research presented in this work.. Choosing different research object - cancerous and non-cancerous cells, male and female reproductive tissues, and various physical parameters of the EMF, such as the frequency of time-varying fields, magnetic induction value, field shape and time of exposure, there were tested effects (negative or positive) of impact, measured by modern methods of molecular biology through biological parameters in in vitro cultures.

PL

Pole elektromagnetyczne, jako czynnik środowiskowy wpływający na komórki i tkanki hodowane in vitro jest tematem badań przedstawionych w niniejszej pracy. Wybierając różne obiekty badawcze - komórki nowotworowe i nienowotworowe, tkanki męskiego i żeńskiego układu rozrodczego oraz różne parametry fizyczne pola elektromagnetycznego, takie jak częstotliwość pól zmiennych w czasie, wartość indukcji magnetycznej, kształt pola i czas ekspozycji, badano efekty (negatywne lub pozytywne) oddziaływania pola, mierzone nowoczesnymi metodami biologii molekularnej w kulturach in vitro.

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Modification of PLGA microspheres’ microstructure for application as cell carriers in modular tissue engineering

Mielan B., Krok-Borkowicz M., Pielichowska K., Pamuła E.

Engineering of Biomaterials

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2017

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Vol. 20, no. 140

7--11

EN

Microspheres (MS) made of resorbable polymer have been proposed as a cell growth support. They may be assembled to form cell constructs or be suspended in hydrogels allowing injection into injury location. High relative surface area of MS provides more efficient cell culture environment than traditional culture on flat substrates (multiwell plates, Petri dishes). In addition, MS structure, topography and surface chemistry can be modified to promote cell adhesion and proliferation. The aim of this study was to obtain resorbable poly(L-lactide-co-glycolide) (PLGA) MS and to modify their properties by changing manufacturing conditions of the oil-in-water emulsification to better control structural and microstructural properties of MS and their biological performance. To this end, water phase was modified by addition of NaCl to change ionic strength, while oil phase by addition of polyethylene glycol (PEG). Microstructural and thermal properties were assessed. Cytocompatibility tests and cell cultures with MG-63 cells were conducted to verify potential relevance of MS as cell carriers. The results showed that it is possible to obtain cytocompatible MS by oil-in-water emulsification method and to control diameter, porosity and crystallinity of MS with the use of additives to oil and/or water phases without negative changes in MS cytocompatibility. The results prove that modification of both phases make it possible to produce MS with desired/controllable properties like surface topography, porosity and crystallinity.