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Analysis of neurotransmitter catecholamines and related amines in human urine and serum by chromatography and capillary electrophoresis with 1, 3, 5, 7-tetramethyl-8-(N-hydroxysuccinimidyl propionic ester)-difluoro-boradiaza-s-indacene

Cao Liwei, Wu Lizhen, Zhong Hailan, Wu Hao, Zhang Siyun, Meng Jianxin, Li Fengyu

Acta Chromatographica

2022

Vol. 34, no. 3

276--286

Two sensitive and effective methods were developed for the detection of catecholamines and related biogenic amines (dopamine, epinephrine, norepinephrine, serotonin, levodopa and tyramine) using high performance liquid chromatography with fluorescence detection and capillary electrophoresis with laser-induced fluorescence detection. A BODIPY fluorescent dye, 1, 3, 5, 70-tetramethyl-8-(N-hydroxysuccinimidyl propionic ester)-difluoroboradiaza-s-indacene was used as pre-column derivatization reagent. The separation and derivatization conditions were optimized in detail. In high performance liquid chromatography with fluorescence detection method, the derivatization reaction was completed at 35 °C for 20 min. At the wavelength of λex/λem = 493 nm/513 nm, dopamine, epinephrine, norepinephrine, and levodopa derivatives achieved baseline separation within 15 min. The limits of detection (S/N = 3) were 1.0, 2.0, 5.0, and 0.5 nmol/L, respectively. In capillary electrophoresis with laser-induced fluorescence detection method, the derivatization reaction was completed at 25 °C for 20 min. Serotonin, tyramine and dopamine derivatives reached baseline separation within 10 min at the wavelength of λex = 473 nm. The limits of detection (S/N = 3) for serotonin, tyramine, and dopamine were 0.3, 0.02, and 0.2 nmol/L, respectively. The amino compounds in human serum and urine samples were detected successfully, and the recoveries were 93.3%–106.7% and 91.0%–103.1%, respectively.

Study of the Inclusion Complexes of Catecholamines with beta-Cyclodextrin by Cyclic Voltammetry

Gao Z., Wen X. -L., Li H. -L.

Polish Journal of Chemistry

2002

Vol. 76 / nr 7

1001-1007

The inclusion complexes of catecholamines (dopamine, DA, and adrenaline, AD) with beta-cyclodextrin (beta-CD) have been studied by cyclic voltammetry (CV) using glassy carbon electrode (GCE) for expanding the potential window. The variations of peak potential and peak current were observed on cyclic voltammograms, when the electroactive guest molecules, DA and AD are complexed with beta-CD. Dissociation constants of cyclodextrin inclusion complexes have been calculated. The experimental results indicate that bothDAandADcan form a 1:1 inclusion complexes with beta-CD in aqueous solutions. It was observed that the light and oxygen sensitivity of the inclusion complex is much lower than those of the free catecholamines, which can be used to improve their storage and handling, and also widen their applications in pharmaceutical industry. Furthermore, it was found that their electrochemical oxidation in the aqueous solutions could be inhibited by beat-CD.