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Biological effects of polymers modified with carbon nanotubes on human osteoblast-like MG 63 cells

Grausova L., Bacakova L., Frączek A., Błażewicz S., Błażewicz M., Pepka M.

Inżynieria Biomateriałów

2006

R. 9, nr 58-60

14-16

The tested materials were represented by a pure terpolymer of polytetrafluorethylene-polyvinyldifluoride-polypropylene (PTFE-PVDF-PP), pure polysulphone (PSU) and PSU modified with single- or multiwalled carbon nanotubes in concentrations of 0.5, 1 or 2 wt%. As control samples, a polystyrene cell culture dish and microscopic glass coverslips were used. The number and viability of human osteoblast-like MG 63 cells in cultures on these materials was detected with a Cell Viability Analyzer (Vi-CELL XR, Beckman Coulter) on 1, 3 and 7 days after seeding. On all tested samples, the cell number was similar or lower than that detected on the control polystyrene dishes. The cell viability on day 1 after seeding was relatively low on PTFE-PVDF-PP and some nanotube-containing samples, ranging from 10 to 100% of living cells, but on day 7 after seeding, it reached at least 90% on all tested samples. The cell spreading area was detected in cells after immunocytochemical staining of beta-actin on day 3 after seeding. In nanotube-containing samples, especially those with multi-walled nanotubes, this area was similar or even larger than that on the control materials. The beta-actin cytoskeleton was well developed in cells on all nanotube-containing materials and similar to that in cells on control surfaces. Thus, it can be concluded that nanotube-containing PSU supports the adhesion and growth of osteoblast-like cells and could be used for construction of bone implants in which the anchorage in the surrounding bone tissue is desirable.

Human osteoblast-like cells MG 63 in cultures on polymer-carbon-alginate composites

Grausova L., Bacakova L., Stodolak E., Blazewicz S., Mikołajczyk S.

Inżynieria Biomateriałów

2005

R. 8, nr 47-53

6--9

Adhesion and growth of human osteoblast-like MG 63 cells (seeding density of 17964 cells/cm2) was studied in cultures on the following 7 groups of artificial materials developed for bone tissue engineering: terpolymer of polypropylene, polytetrafluorethylene and polyvinyldlfluoride (CPO), terpolymer reinforced wlth carbon fibres (CP4) or carbon fabric (CP5), terpolymer reinforced with carbon fibres and with pores created by addition and dissolution of alginate fibres (CP6) or powder (CP7), terpolymer reinforced with carbon fibres and containing the alginate powder (CP8) or fibres (CP9). On day 1 and 3 after seeding, the numbers of MG63 cells on all tested materials was similar to the values obtained on the control polystyrene culture dish (PS). However, on day 7, the cell number, ranging from 17766±3180 to 67002±6850 cells/cm2, increased in the following order: CP0