Wyniki wyszukiwania - BazTech

Ograniczanie wyników

1 Wiadomości Chemiczne

1 2025

Znaleziono wyników: 1

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: cancer biomarkers

Sortuj według:

Ogranicz wyniki do:

Badania spektroskopowe i mikroskopia sił atomowych komórek i tkanek, prawidłowych i nowotworowych ludzkiego przewodu pokarmowego

Beton-Mysur Karolina, Kopeć Monika, Surmacki Jakub, Jarota Arkadiusz, Brożek-Płuska Beata

Wiadomości Chemiczne

2025

[Z] 79, 11-12

1169--1220

This paper presents a comprehensive study of the biochemical and nanomechanical properties of normal and cancerous cells and tissues of the human gastrointestinal tract. The research combined Raman spectroscopy and imaging, fluorescence spectroscopy, and atomic force microscopy to achieve a multidimensional characterization of cellular and tissue structures. Experiments were conducted under physiological conditions, as well as in cells exposed to oxidative stress and subsequently supplemented with antioxidants, including vitamins and carotenoids. The impact of fatty acid supplementation on cellular morphology and lipid metabolism was examined, alongside the effects of HMG-CoA reductase inhibitors on cholesterol biosynthesis, using both natural and synthetic statins (mevastatin, lovastatin, simvastatin). These compounds demonstrated a dual effect, combining anticancer activity with hypocholesterolemic action. Atomic force microscopy enabled the evaluation of nanomechanical properties in physiological and supplemented cells, with Young’s modulusidentified as a potential mechanomarker capable of distinguishing between different cellular states. Interactions between photosensitizers and cells were investigated using tetrasulfonated aluminum phthalocyanine in normal colon cells. Fluorescence imaging revealed preferential accumulation of the photosensitizer within lipid-rich structures such as the endoplasmic reticulum and lipid droplets. Femtosecond laser spectroscopy of solutions in PBS indicated that dimerization plays a crucial role in modulating the photophysical dynamics of phthalocyanine systems below the critical micellization concentration. Complementary UV-Vis measurements confirmed the absence of dimerization in AlPcS4 solutions prepared in PBS. The central hypothesis of the study proposed that spectroscopic and nanomechanical biomarkers can reliably differentiate normal from cancerous gastrointestinal cells and tissues with high sensitivity and specificity. This was validated using colon and stomach samples, where Raman signatures of proteins, lipids, and nucleic acids served as robust discriminators. Cluster Analysis and Basis Analysis enabled the visualization of cellular substructures and the spatial distribution of biochemical constituents, while fluorescence microscopy further highlighted nuclear and lipid features in normal and cancerous colon cells, including those treated with statins and fatty acids. Quantitative analysis of lipid structures per cell confirmed the inhibitory effect of statins on HMG-CoA reductase. Cell viability assays (XTT) established effective supplementation concentrations and assessed survival under external stress conditions. Chemometric methods, including PCA and PLS-DA, facilitated the objective identification of Raman-based biomarkers distinguishing normal from malignant cells and tissues. Statistical validation with ANOVA and Pearson’s correlation coefficients confirmed both the significance of the findings and the spectral similarity patterns between untreated and supplemented cells.