Wyniki wyszukiwania - BazTech

Ograniczanie wyników

2 Engineering of Biomaterials

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: calcium carbonate microparticles

Sortuj według:

Ogranicz wyniki do:

Optimization of the calcium carbonate particles manufacturing process by the precipitation method and biological evaluation with MG-63 cells

Pudełko-Prażuch Iwona, Wojtanek Karolina, Pamuła Elżbieta

Engineering of Biomaterials

2023

vol. 26, no. 170

20--27

As a natural mineral, calcium carbonate (CaCO3) is widely investigated for various medical applications. It is a biocompatible material characterized by high degradation rate and great osteoconductivity. Many researchers evaluate CaCO3 in the form of particles as a candidate for use in drug delivery systems. In this study we present an optimization of the process of producing CaCO3 particles by the precipitation method with the use of combinations of different time of ultrasound treatment and surfactant concentrations used. Depending on the synthesis conditions, various sizes of particles were fabricated. The particles were loaded with sodium alendronate (Aln, 5% or 10% by weight) with a relatively high encapsulation efficiency between 40 and 50%, depending on the amount of Aln added and the drug loading of approximately 9% for both cases. MG-63 osteoblast-like cells were contacted with 10% wt./vol extracts of fabricated particles to assess their cytotoxicity. None of the extracts investigated was found to be cytotoxic. Furthermore, an in vitro study in direct contact of MG-63 cells with particles suspended in culture medium was performed. The results showed that the fabricated particles are cytocompatible with osteoblast-like MG-63 cells. However, the higher the concentration of the particle suspension and the greater the amount of alendronate present in the solution, the lower the metabolic activity of the cells was measured. The presented method of CaCO3 particles manufacturing is simple, cost-effective, and allows one to fabricate particles of the required size and shape that are cytocompatible with MG-63 cells in defined concentrations of particle suspensions.

Composite scaffolds enriched with calcium carbonate microparticles loaded with epigallocatechin gallate for bone tissue regeneration

Pietryga Krzysztof, Panaite Anca-Alexandra, Pamuła Elżbieta

Engineering of Biomaterials

2022

vol. 25, no. 166

12--21

There is a need to develop advanced multifunctional scaffolds for the treatment of bone tissue lesions, which apart from providing support for infiltrating cells could assure the delivery of drugs or biologically active molecules enhancing bone formation. We developed composite scaffolds for bone tissue engineering based on gellan gum (GG) and gelatin (Gel) hydrogel enriched with epigallocatechin gallate (EGCG) loaded CaCO3 microparticles and subjected to enzymatic mineralization with calcium phosphate (CaP). The method of manufacturing CaCO3 microparticles was optimized. The EGCG-loaded microparticles were smaller than those unloaded, and the release of EGCG was prolonged for up to 14 days, as shown by the Folin-Ciocalteu test. The particles reduced the viability of the MG-63 cells as compared to the control. However, when they were loaded with EGCG, their cytotoxicity was reduced. The particles were suspended in a GG/Gel hydrogel containing alkaline phosphatase (ALP), soaked in calcium glycerophosphate (CaGP) solution to create CaP deposits, and submitted to freeze-drying, in order to produce a porous scaffold. The microstructure of the scaffolds was characterized by optical and scanning electron microscopy and showed that the size of the pores corresponds to that of the spongy bone. In vitro tests with MG-63 cells confirmed that mineralized scaffolds support cell adhesion and growth to a higher extent than nonmineralized ones.