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Simultaneously measure the concentrations of busulfan and phenytoin in human plasma using an HPLC-MS/MS method: Application to the TDM for children underwent hematological stem cell transplantation

Sun Ning, Li Zhuo, Zhang Meng, He Huan, Zhao Libo, Mei Dong, Zhu Guanghua, Wang Xiaoling

Acta Chromatographica

2023

Vol. 35, no. 4

302--309

In this work, a simple and rapid high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed and validated to carry out the simultaneous measurement of busulfan (BU) and phenytoin (PHT) in the plasma of children. In this method, plasma sample could be prepared by one-step protein precipitation using 1 mL of methanol/water (1:1, v/v). After centrifugation (14,500 rpm, 5 min, 4 °C), 10 μL of the supernatant was injected into a Hypersil Gold C18 column (150 × 2.1 mm, 5 μm, Thermo Fisher Scientific) for separation by gradient elution. Quantification was carried out using multiple reactions monitoring (MRM) under positive scan mode. In the method verification, the calibration curves of BU and PHT showed satisfactory linearity (r > 0.99) at the concentration ranging from 0.02 to 20 μg mL⁻¹. The accuracy and precision were tested at four concentration levels (including the LLOQ level) with the relative error (RE) ranging from −0.80% to 11.45% and coefficient of variation (CV) between 0.93% and 7.74%. There was no pronounced matrix effect to interfere with the quantitative analysis. Compared to determine BU and PHT using two individual methods, less pre-treatment process, labor and blood sample volume are required in this proposed method. Finally, this method was successfully applied to the therapeutic drug monitoring of BU and PHT for children underwent hematological stem cell transplantation.

Effect of static magnetic field and Busulfan on HL-60 cell apoptosis

Sztafrowski D., Kuliczkowski K., Jaźwiec B., Gumiela J.

Przegląd Elektrotechniczny

2018

R. 94, nr 1

109--112

The article presents the results of the in vitro studies which were carried out to determine the effect of constant magnetic field (SMF - static magnetic field) and organic chemical called Busulfan on the level of apoptosis of human leukemia cells HL-60 in the study populations. The mortality increase of cells HL-60 in the case of a constant magnetic field and busulfan with respect to groups of cells treated with cytostatic only has been discovered. The use of a constant magnetic field as a supporting factor in therapy with selected cytostatic has been proposed.

Artykuł prezentuje wyniki badań in vitro których przeprowadzenie miało na celu ustalenie wpływu stałego pola magnetycznego (SMF – ang. Static Magnetic Field) oraz związku chemicznego o nazwie Busulfan na poziom apoptozy komórek białaczki ludzkiej HL-60 w badanych populacjach. Stwierdzono wzrost śmiertelności komórek HL-60 w przypadku zastosowania stałego pola magnetycznego i Busulfanu względem grupy komórek poddanych działaniu samego cytostatyku. Postuluje się zastosowanie stałego pola magnetycznego jako czynnika wspomagającego terapię wybranymi cytostatykami.