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Formation of the Population of Micromycetes in the Leaf Microbiome of Cereal Cultures Using Different Plant Cultivation Technologies

Beznosko Iryna, Havryliuk Liliіa, Mazur Svetlana, Gorgan Tetiana, Mosiychuk Iryna, Bashta Olena, Kichigina Olga, Turovnik Julia

Journal of Ecological Engineering

2023

Vol. 24, nr 11

236--248

The selection of cereal crops varieties as a factor in the regulation of the phytopathogenic microbiome in agrocenoses is an actual direction of the research. Cultivation of such varieties leads to a decrease in the level of biological pollution in agrocenoses and increases the quality as well as safety of agricultural products in agroecosystems. Therefore, the influence of the environmental factors (including abiotic, biotic, anthropogenic, and other) on the formation of micromycete populations in the leaf microbiome of grain crops using different plant cultivation technologies has been thoroughly studied earlier. The results of the selection the plant varieties by the indicators of influence on their population density, the frequency of the occurrence, and the intensity of the micromycete sporulation, were presented in this article. Vegetative organs of plants of the cereal crops (including the oats of Parliamentsky variety, Tembre variety, and spring barley Salomi and Sebastian varieties) were selected in the following phases: tillering, stem stage of growth, and earing. It was determined that using the traditional and organic technologies of plant cultivation in the leaf microbiome of Tembre oats and Salomi variety spring barley, the population density, the frequency of the occurrence of micromycete species, and their sporulation intensity were significantly lower compared to the plants of Parliamentsky oat and Sebastian spring barley. This shows that the cultivation of the cereal crops varieties capable of restraining the formation of micromycete on an ecologically safe level will result in a decrease in the level of biological pollution of agrocenoses and increase the biosafety of plants.

Biobezpieczeństwo w laboratorium mikrobiologicznym

Marczewska J.

Laboratorium Medyczne

2018

nr 1

52--56

Ograniczenie bioryzyka w laboratorium mikrobiologicznym polega na zapobieganiu wystąpienia zakażenia czynnikami biologicznymi. Należy zapewnić stałą ochronę pracownika i środowiska przed czynnikami zakaźnymi obecnymi w badanych materiałach. W celu redukcji lub eliminacji narażenia na potencjalne czynniki niebezpieczne powinny być stosowane środki bezpieczeństwa. Do najważniejszych należą właściwie zaplanowane pomieszczenia oraz stosowanie odpowiednich zabezpieczeń chroniących przed wydostaniem się czynników zakaźnych, w sposób niekontrolowany, z materiału biologicznego.

The reduction of biohazard in a microbiological laboratory consists in the prevention of infection. The continuous protection of workers and the environment against infectious agents present in the tested materials should be ensured. Safety measures should be taken in order to reduce or eliminate exposure to potential hazardous materials. The most important measures include adequately designed facilities and the means disabling the uncontrolled escape of infectious agents from a biological material.

Safety of workers exposed to harmful airborne bioaerosols – legal status and innovations

Majchrzycka K., Okrasa M., Szulc J., Gutarowska B.

Biotechnology and Food Science

2017

Vol. 81, nr 2

159--168

Filtering respiratory protective equipment is commonly used for protection against bioaerosols in industrial workplaces. However, the EU legislation does not specify the requirements for its antimicrobial properties. The result is that in the market there is no equipment available that would ensure complete safety of workers exposed to inhalation of harmful bioaerosols. This is particularly important in the case of equipment intended for multiple and long-term use. The aim of this study was to develop an innovative filtering half mask for the protection of respiratory tract that would have confirmed antimicrobial properties. The half mask was tested for compliance with EN 149:2001+A1:2009, and the survival rate of microorganisms in nonwovens was determined (E. coli bacteria and A. niger moulds). The reduction in the number of E. coli bacteria was 98.69% and the reduction in the number of A. niger moulds was 67.68% after 32 h of storage under conditions simulating the conditions of use of the equipment. It was found that the biocidal activity of the half mask is sufficient to ensure a significant reduction in the number of microorganisms during its long-term use in the workplace. This in turn means that continuous disinfection of respiratory protective equipment would be maintained even without employees’ intervention, which is not possible in case of standard respiratory protective equipment.