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1

The Effects of Water Extracts from Tansy on Pea Leaf Weevil and Black Bean Aphid

Kwiecień Natalia, Gospodarek Janina, Boligłowa Elżbieta

Journal of Ecological Engineering

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2020

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Vol. 21, nr 3

220--227

EN

The purpose of the research conducted was to define the impact of the various aqueous extract concentrations, prepared from the dried (in concentrations 2%, 5% and 10%) and fresh (in concentrations 10%, 20% and 30%) tansy (Tanacetum vulgare L.) mass, on the feeding of pea leaf weevil (Sitona lineatus L.), and the mortality rate of black bean aphid (Aphis fabae Scop.). The studies showed that in order to limit the feeding of pea leaf weevils considerably, it is necessary to use at least 5% extract of the dried tansy mass or 20% extract of the fresh tansy mass. The impeding effect on the feeding of pea leaf weevils was correlated positively with the extract concentration. The insecticide effect of the aqueous extract of tansy on black bean aphid was revealed not earlier than after 12 hours with the use of at least 20% extract of the fresh tansy mass and 5% extract of the dried mass (only in relation to aphid larvae). Nevertheless, the extracts of lower concentration also demonstrated the insecticide effectiveness although delayed (36–60 hours after the application). As compared to other studies conducted according to the same methodology, it was demonstrated that the tansy aqueous extracts are characteristic for a high deterrent activity in relation to the pea leaf weevil beetles (higher than the extracts of absinthe and similar to the extracts of pepper mint and sage) and a quite high aphicidal activity (higher effectiveness than in the case of the aqueous extracts of lemon balm but lower than the extracts of tarragon and absinthe).

2

Biological Control as an Alternative Method of Protecting Crops Against Fungal Pathogens

Kisiel Anna

Rocznik Ochrona Środowiska

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2019

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Tom 21, cz. 2

1366--1377

EN

Progressive degradation of the environment caused, among others, by the excessive use of chemical pesticides forces us to look for alternative methods of protecting crops against pathogens. Definitely beneficial for the environment, but also, as confirmed by numerous studies, the use of biological control mechanisms can be an effective solution. The bacteria can inhibit the growth of fungi through the production of enzymes that lyse their cell walls, such as chitinases and glucanases, but also by limiting the availability of microelements important to growth, such as iron, by chelating them to siderophores, and finally producing antibiotics and secondary metabolites, like 2,4-DAPG. The present study examined 16 strains of bacteria isolated from Medicago sativa rhizosphere for their suitability for the control of fungal pathogens such as Fusarium culmorum and Phoma medicaginis. Among of bacteria were strains belonging to the family Bacillaceae (genus Bacillus, Lysinibacillus and Paenibacillus), the family Pseudomonadaceae (genus Pseudomonas), the family Xantomonadaceae (genus Stenotrophomonas) of the Enterobacteriaceae family (genus Citrobacter, Leclercia and Raoultella) and of the family Rhizobiaceae (genus Sinorhizobium). In vitro, both on solid and liquid media, all bacterial strains were able to limit the growth of Fusarium culmorum and Phoma medicaginis. The effective inhibitors of Fusarium culmorum Cul-3 were Roultella planticola KK 8a, Pseudomonas corrugata KK 12, and both strains belonging to Stenotrophomonas maltophilia KK 8b and KK 9b. Limiting the development of the second Medicago pathogen, Phoma medicaginis, was the most effective after the use of Pseudomonas corrugata KK 7, Leclercia adecarboxylata KK 6 and Pseudomonas brassicacearum KK 5. By studying the mechanisms that may be potential for bacteria to inhibit the growth of fungi was tested production of lytic enzymes the cell walls, and siderophores. All bacterial strains showed β-1,3-glucanase activity in the range of 2.3 to 72.5 μmol glucose /mg protein/min. Five strains showed chitinolytic activity, with Stenotrophomonas maltophilia KK 8b being the most active. Thirteen of the 16 analyzed strains of rhizobacteria were able to produce iron chelating compounds, siderophores. In addition, the presence of the phlD gene was confirmed in Pseudomonas brassicacearum KK 5 and P. corrugata KK 12, which may indicate the production of 2,4-DAPG. The results obtained indicate that the bacteria isolated from the rhizosphere have the potential for biological control of fungal pathogens, which limit the plant cultivation, using various mechanisms. The use of rhizobacteria as biopesticides is an environmentally friendly alternative to chemical plant protection products.

PL

Postępująca degradacja środowiska spowodowana między innymi nadmiernym wykorzystywaniem chemicznych środków ochrony roślin zmusza nas do poszukiwania alternatywnych metod zabezpieczania upraw przed patogenami. Zdecydowanie korzystnym dla środowiska ale również, jak potwierdzają liczne badania, skutecznym rozwiązaniem może być wykorzystanie mechanizmów zwalczania biologicznego. Bakterie mogą ograniczyć rozwój patogenów grzybowych poprzez produkcję enzymów lizujących ich ściany komórkowe, takich jak chitynazy i glukanazy ale także poprzez ograniczenie dostępności ważnych dla ich wzrostu mikroelementów, jak żelazo, chelatując je do sideroforów, czy wreszcie produkując grzybobójcze antybiotyki i metabolity wtórne, jak 2,4-DAPG.W niniejszych badaniach przeanalizowano 16 szczepów bakterii wyizolowanych z ryzosfery Medicago sativa pod kątem ich przydatności do zwalczania patogenów grzybowych takich jak Fusarium culmorum i Phoma medicaginis. Wśród bakterii były szczepy należące do rodziny Bacillaceae (rodzaju Bacillus, Lysinibacillus i Paenibacillus), z rodziny Pseudomonadaceae (rodzaju Pseudomonas), z rodziny Xantomonadaceae (rodzaju Stenotrophomonas), z rodziny Enterobacteriaceae (rodzaju Citrobacter, Leclercia i Raoultella) oraz z rodziny Rhizobiaceae (rodzaju Sinorhizobium). W warunkach in vitro zarówno na pożywkach stałych jak i płynnych wszystkie analizowane szczepy bakterii były zdolne do ograniczenia wzrostu Fusarium culmorum oraz Phoma medicaginis. Do skutecznych inhibitorów rozwoju Fusarium culmorum Cul 3 należy zaliczyć szczepy Roultella planticola KK 8a, Pseudomonas corrugata KK 12, oraz oba szczepy należące do Stenotrophomonas maltophilia KK 8b i KK 9b. Ograniczenie rozwoju drugiego patogena Medicago, tj. grzyba Phoma medicaginis było najskuteczniejsze po zastosowaniu szczepów Pseudomonas corrugata KK 7, Leclercia adecarboxylata KK 6 i Pseudomonas brassicacearum KK 5. Badając mechanizmy, które mogą stać za potencjałem bakterii do hamowania wzrostu grzybów sprawdzono produkcję enzymów lizujących ściany komórkowe oraz sideroforów. Wszystkie szczepy bakterii wykazywały aktywność β-1,3-glukanazy w zakresie od 2,3 do 72,5 μmol glukozy/mg białka/min. Pięć szczepów wykazało aktywność chitynolityczną, największą aktywnością charakteryzował się szczep Stenotrophomonas maltophilia KK 8b. Trzynaście z 16 analizowanych szczepów ryzobakterii było zdolnych do produkcji związków chelatujących żelazo, sideroforów. Ponadto u Pseudomonas brassicacearum KK 5 i P. corrugata KK 12 potwierdzono obecnośc genu phlD, co może świadczyć o produkcji 2,4-DAPG. Jak wynika z przeprowadzonych badań bakterie izolowane z ryzosfery posiadają potencjał do zwalczania patogenów grzybowych ograniczających uprawy, wykorzystując do tego szereg mechanizmów. Zastosowanie ryzobakterii jako biopestycydy jest przyjazną dla środowiska alternatywą dla chemicznych środków ochrony roślin.

3

The Effects of Water Extracts from Lemon Balm on Pea Leaf Weevil and Black Bean Aphid Behaviour

Rusin M., Gospodarek J.

Journal of Ecological Engineering

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2018

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Vol. 19, nr 6

139--145

EN

The objective of this study was to determine the effects of various concentrations of water extracts prepared from the fresh or dry matter of lemon balm on Sitona lineatus L. and Aphis fabae Scop. behaviour. The assessment pertaining to the feeding intensity of beetles was carried out by measuring the surface of feeds caused by S. lineatus. While examining the effect of extracts on A. fabae, the mortality of wingless female and aphid larvae was determined. In the studies on the olfactory reaction glass olfactometer "Y tube" and 4 armed arena olfactometer were used. The results of the experiment showed that the water extract prepared from dry matter of lemon balm with 2% concentration limited the feeding of both female and male of S. lineatus. The increase in the mortality of the black bean aphid females and larvae was obtained only after applying the extracts from fresh and dry matter at highest concentrations. The evident deterrent reaction of the odour substances obtained from the lemon balm plants towards the beetles of S. lineatus, could find application in ecological farms via introducing the plant as an accompanying crop to the main crops. The winged individuals of A. fabae did not react to the abovementioned factor.

4

The Effects of Water Extracts from Lemon Balm on Colorado Potato Beetle Behaviour

Rusin M., Gospodarek J.

Journal of Ecological Engineering

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2018

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Vol. 19, nr 5

122--127

EN

The objective of this study was to determine the effects of various concentrations of water extracts prepared from the fresh or dry mass of lemon balm on the feeding of Colorado potato beetle larvae in L2 and L4 stages. The extracts from the dry matter were prepared at the concentrations of 2%, 5% and 10% and from the fresh matter at the concentrations of 10%, 20% and 30%. The feeding intensity assessment was conducted by dipping leaves of potato in respective solutions of the extracts and determining the mass of food consumed by larvae, as well as the changes of larvae body weight. In the studies on the olfactory reaction of adult insects, a glass “Y-tube” olfactometer was used. The feeding by L4 larvae of Colorado potato beetle was effectively limited by the extract prepared from the dry matter of lemon balm with 10% concentration. The larvae at L2 stage turned out to be more susceptible to the effects of extracts – the extracts in almost all used concentrations limited the feeding of the studied pest. The body weight gain in L2 and L4 larvae of Colorado potato beetle were effectively limited by the extracts prepared from the dry plants of lemon balm in as low concentration as 5%. However, the effect of extracts prepared from the fresh matter of lemon balm plants was effective in limiting the body weight gain of L4 larvae at the minimum concentration of 20%, whereas for L4 larvae the effective concentration was 30%. The evident deterrent reaction of odour substances originating from lemon balm plants towards the females of Colorado potato beetle was found. The males did not react to the above-mentioned factor.

5

Biocontrol of Common Vole Populations by Avian Predators Versus Rodenticide Application

Machar I., Harmacek J., Vrublova K., Filippovova J., Brus J.

Polish Journal of Ecology

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2017

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Vol. 65, nr 3

434--444

EN

This paper presents results of a study focused on using the increased predation pressure of avian raptors for biocontrol of local populations of the common vole (Microtus arvalis) in Haná (Czech Republic), a region of traditional agriculture in central Europe. Five raptor perches per hectare were installed on fields (total number of perches installed per year was 625) during two vole outbreaks in 2005/2006 and 2009/2010. The importance of the installed perches for the abundance of raptors during both outbreaks was evaluated based on the overall raptor counting along a transect. The results imply that supporting aggregations of raptors on agricultural arable land by means of installed artificial perches can increase the predation pressure on M. arvalis at the onset and during its population outbreaks. The density of raptors was low in fields without installed perches, although the local vole population was reaching its peak densities (2100 active burrows per hectare). In contrast, the density of raptors in fields with installed perches was markedly high. The results showed that the cost of biological control applied to agricultural land with an ongoing vole outbreak may sum up to approx. 50% of the rodenticide application costs (with equal efficiencies of both methods reducing the common vole abundance below the economic injury level).

6

The effect of water extract of chamomile (Matricaria chamomilla L.) on feeding the pea leaf weevil (Sitona lineatus L.) beetles

Biniaś B., Gospodarek J.

Journal of Research and Applications in Agricultural Engineering

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2017

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Vol. 62, nr 3

37--41

EN

The aim of the study was to determine the effects of aquatic extracts of dry matter of chamomile (Matricaria chamomilla L.) in concentrations 2, 5 and 10% and fresh parts of this plant in concentrations 10%, 20% and 30% on feeding the females and males of pea leaf weevil (Sitona lineatus L.) beetles on broad bean (Vicia faba L.) leaves, variety “Bartek”. In the study, the 9 laboratory observations at 12-hour intervals were carried out. The leaf surface consumed by adults of Sitona lineatus was measured. The observations were performed in 6 replicates with the division into males and females. Additionally, the palatability index and an absolute deterrence index were calculated. The feeding of pea leaf weevil females was effectively reduced by applying a dried chamomile extract at a 10% concentration. The feeding of S. lineatus males was reduced to a very small degree, mainly by extracts prepared from dried plant matter. Extracts prepared from fresh chamomile matter did not exhibit any visible inhibitory effect on S. lineatus feeding.

PL

Celem badań było określenie wpływu wodnych wyciągów z suszu rumianku pospolitego (Matricaria chamomilla L.) w stężeniach 2, 5 i 10% oraz ze świeżych części rośliny w stężeniach 10, 20 i 30 na żerowanie samic i samców chrząszczy oprzędzika pręgowanego (Sitona lineatus L.) na liściach bobu (Vicia faba L.) odmiany „Bartek”. W badaniach przeprowadzono 9 obserwacji laboratoryjnych, w odstępach 12-godzinnych. Mierzono powierzchnię wyżerek zatokowych, powodowanych przez dorosłe osobniki Sitona lineatus. Obserwacje przeprowadzono w 6 powtórzeniach z podziałem na samce i samice. Dodatkowo przeprowadzono analizę wskaźnika smakowitości oraz bezwzględnego współczynnika deterentności. Skuteczne ograniczenie żerowania samic oprzędzika pręgowanego, uzyskano po zastosowaniu wyciągu z suszu rumianku pospolitego w stężeniu 10%. Żerowanie samców S. lineatus było ograniczane w bardzo małym stopniu, przede wszystkim przez wyciągi na bazie suszu. Wyciągi ze świeżej masy rumianku nie wykazały wyraźnego działania hamujacego żerowanie chrząszczy S. lineatus.

7

Effect of Water Extract From Mint on Selected Crop Pests Feeding and Survival

Biniaś B., Gospodarek J., Rusin M.

Journal of Ecological Engineering

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2017

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Vol. 18, nr 2

119--128

EN

The aim of the study was to determine the effect of aqueous extracts of dried mint (Mentha piperita L.) in concentrations of 2%, 5% and 10%, and fresh parts of this plant, in concentrations of 10%, 20% and 30% on feeding of pea leaf weevil (Sitona lineatus L.) and survival of black bean aphid (Aphis fabae Scop.) on the leaves of broad bean (Vicia faba L.), variety “Bartek”. In the studies of the effects of aqueous extracts of mint on the dynamics of Sitona lineatus feeding on the beans leaves, 9 laboratory’s observations in 12-hour intervals was made. Leaves injuries on their edge surface caused by adult individuals were measured. Observations were carried out in 6 replicates divided into males and females. In these researches on impact of water extracts from mint on mortality of wingless females and larvae of black bean aphids, 14 laboratory’s observations in the 8-hour intervals was made. Aqueous extracts of dry and fresh peppermint significantly limited the feeding of pea leaf weevil females, and the extracts from dried material were more effective. Extracts from peppermint caused mortality of black bean aphid larvae compared to wingless females to a greater extent, and the higher the concentration of the extract, the more beneficial effect was observed. An extract from dried material at a concentration of 2% was too weak to effectively combat the larvae of A. fabae, while in the case of aphid females, an effectiveness was only demonstrated for the extracts at the highest concentrations, i.e., 10% of dried and 30% of fresh peppermint.

8

Effect of Water Extract from Chamomile on Black Bean Aphid and Colorado Potato Beetle

Biniaś B., Gospodarek J.

Journal of Ecological Engineering

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2017

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Vol. 18, nr 3

118--124

EN

The aim of the study was to determine the effect of aqueous extracts of dried chamomille (Matricaria chamomilla L.), in concentrations of 2%, 5% and 10%, and fresh parts of this plant, in concentrations of 10%, 20% and 30% on survival of black bean aphid (Aphis fabae Scop.) on the leaves of broad bean (Vicia faba L.) variety “Bartek” and on feeding of Colorado potato beetle (Leptinotarsa decemlineata Say.) on the leaves of potato (Solanum tuberosum L.) variety “Bellarosa”. In these researches on impact of water extracts from chamomille on mortality of wingless females and larvae of black bean aphid, 15 laboratory’s observations in the 8-hour intervals was made. In the studies of the effects of aqueous extracts of chamomille on the dynamics of Leptinotarsa decemlineata feeding on the potato leaves, 4 laboratory’s observations in 12-hour intervals was made. Observations were carried out in 6 replicates divided into L2 and L4 larvae stages. Aquatic extracts of chamomile limited the survival of black bean aphid larvae and wingless females – the higher the concentration, the stronger the effects of the extract, where young insects were found to be more susceptible. Extracts of the dried plant whose concentration was below 5% and extracts of the fresh plant whose concentration was below 20% were found to be ineffective against black bean aphids. In all the analysed concentrations the aquatic extracts of chamomile were found to be ineffective against L2 and L4 larvae of Colorado potato beetle.

9

Biologiczna kontrola Rhizoctonia solani AG 2-2IIIB przez metabolity Bacillus subtilis

Nagrodzka K., Moliszewska E., Grata K., Nabrdalik M.

Proceedings of ECOpole

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2016

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Vol. 10, No. 2

741--748

PL

Celem doświadczenia było określenie aktywności metabolitów produkowanych przez bakteryjny szczep Bacillus subtilis wobec izolatu Rhizoctonia solani należącego do grupy AG 2-2IIIB. Antagonistyczne właściwości metabolitów B. subtilis były oceniane w kulturach płytkowych na podłożu Czapka po 6, 24 i 48 godzinach hodowli w temperaturze 30 i 37ºC. Wpływ metabolitów wytwarzanych przez B. subtilis na wzrost R. solani AG 2-2IIIB przedstawiono w postaci współczynnika tempa wzrostu liniowego grzyba. Uzyskane wyniki wykazały, że na fungistatyczną aktywność metabolitów B. subtilis wobec R. solani AG 2-2IIIB wpływa zarówno czas, jak i temperatura inkubacji bakterii. Wzrost grzybni był najmocniej hamowany przez metabolity uzyskane po 6-godzinnej hodowli w temp. 37ºC.

EN

The aim of the experiment was to determine the activity of metabolites produced by bacteria Bacillus subtilis Kg against fungus isolate of Rhizoctonia solani ID 105 belonging to the anastomosis group AG 2-2IIIB. The antagonist properties of B. subtilis Kg metabolites were evaluated with a culture-plate method on Czapek growth media for B. subtilis Kg cultures after 6, 24 and 48 hours of culture at temp. 30 and 37°C. The impact of B. subtilis Kg metabolites on the growth of R. solani AG 2-2IIIB was shown as a growth rate index of the fungus. The results showed that on the fungistatic activity of the metabolites of B. subtilis against R. solani AG 2-2IIIB affects both the time and temperature of the bacterial culture. Mycelial growth was most strongly inhibited by the metabolites obtained after 6 hours cultivation at 37°C.

10

Effect of aqueous extract of St. John’s wort (Hypericum perforatum L.) on the Colorado potato beetle (Leptinotarsa decemlineata Say) behaviour

Biniaś B., Gospodarek J., Rusin M.

Journal of Research and Applications in Agricultural Engineering

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2016

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Vol. 61, nr 3

25--29

EN

The object of the laboratory experiment was to investigate the effect of aqueous extracts of dried St. John’s Wort (Hypericum perforatum L.) in concentrations of 2%, 5% and 10%, and fresh parts of this plant, in concentrations of 10%, 20% and 30% on feeding of larvae of Colorado potato beetle (Leptinotarsa decemlineata Say) at L3 and L4 stages. We examined the mass of eaten potato leaves treated with different extracts after 24, 48 and 60 hours from the start of the experiment. Additionally, reactions of adult potato beetle individuals to the scent of the above mentioned plant was tested with the use of an olfactometer. Aqueous extracts from St. John’s wort exhibit inhibitory effect on feeding of potato beetle larvae at a concentration of at least 5% and 10% for dried plant and 20-30% for fresh plant weight and the effect is stronger for L3 larvae than L4 larvae. However, the comparatively low values of the absolute deterrence index suggest limited possibility to use aqueous extracts from the plant for the inhibition of potato beetle larvae feeding. Tests with the use of olfactometer did not exhibit a deterrent reaction of aromatic substances from St. John’s wort to adult individuals of potato beetle (both females and males).

PL

Celem doświadczenia laboratoryjnego było zbadanie wpływu wodnych wyciągów z suszu dziurawca zwyczajnego (Hypericum perforatum L.) w stężeniach 2, 5 i 10% oraz ze świeżych części tej rośliny w stężeniach 10, 20 i 30% na żerowanie larw L3 i L4 stonki ziemniaczanej (Leptinotarsa decemlineata Say). Oceniano masę zjadanych przez larwy stonki ziemniaczanej liści ziemniaka potraktowanych poszczególnymi wyciągami w 24, 48 i 60 godzinie od założenia doświadczenia. Ponadto przy użyciu olfaktometru zbadano reakcje postaci dorosłych stonki ziemniaczanej na zapach wyżej wymienionej rośliny. Wodne wyciągi z dziurawca pospolitego wykazują działanie ograniczające żerowanie larw stonki ziemniaczanej przy stężeniu co najmniej 5 i 10% dla suszu oraz 20-30% dla świeżej masy roślinnej, przy czym efekt jest silniejszy wobec larw L3, aniżeli larw L4. Stosunkowo niskie wartości bezwględnego wskaźnika deterentności sugerują jednak ograniczone możliwości wykorzystania wodnych wyciągów z tej rośliny dla hamowania żerowania larw stonki ziemniaczanej. Badania z wykorzystaniem olfaktometru nie wykazały reakcji odstraszającej substancji zapachowych pochodzących od dziurawca zwyczajnego w stosunku do chrząszczy stonki ziemniaczanej.

11

The Effect of Water Extracts From Winter Savory on Black Bean Aphid Mortality

Rusin M., Gospodarek J., Biniaś B.

Journal of Ecological Engineering

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2016

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Vol. 17, nr 1

101--105

EN

The aim of this study was to determine the effect of water extracts prepared from fresh and dry matter of winter savory (Satureja montana L.) on mortality of wingless females and larvae of black bean aphid (Aphis fabae Scop.). The experiment was conducted in the laboratory, in six replicates. Dry extracts were prepared at concentration of 2%, 5% and 10%, while the fresh plant at concentration of 10%, 20% and 30%. Stomach poisoning of extracts was determined by soaking broad bean leaves in the respective solutions, and then determining mortality of wingless female and larvae feeding on leaves thus prepared at 12 hour intervals. The results of the experiment showed that the extract prepared from dry matter at the highest concentration (10%), as well as the extracts from fresh matter at concentration of 20% and 30% contributed to an increase in mortality of wingless female of black bean aphid. Meanwhile, extracts prepared from both dry and fresh matter at two highest concentrations caused an increase in mortality of larvae of this pest. Furthermore, with increasing concentrations of analysed extracts prepared from both fresh and dry matter of winter savory, their negative effect on wingless females and larvae usually increase.

12

The Effect of Water Extract From Wild Thyme on Colorado Potato Beetle Feeding

Rusin M., Gospodarek J., Biniaś B.

Journal of Ecological Engineering

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2016

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Vol. 17, nr 4

197--202

EN

The aim of this study was to determine the effect of water extracts prepared from fresh and dry matter of wild thyme (Thymus serpyllum L.) on feeding adults and larvae of Colorado potato beetle (Leptinotarsa decemlineata Say.). The experiment was conducted in the laboratory, in six replicates. Dry extracts were prepared at concentration of 2%, 5% and 10%, while the fresh plant at concentration of 10%, 20% and 30%. Feeding intensity assessment was conducted by dipping leaves of potato in respective solutions of the extracts and determining the mass of food consumed by adults and larvae, and changes of larvae body weight once daily. In addition, absolute deterrence index and palatability index were calculated. The results of the experiment showed that for an efficient limitation of the feeding adult Colorado potato beetles, an extract from dry matter of wild thyme with a concentration of at least 10% should be used. L4 larvae appear to be considerably more susceptible to the effect of T. serpyllum extract. In their case, an efficient limitation of the feeding, as well as body weight gain can be obtained using extracts from both dry and fresh matter, at concentrations of over 5% for dry matter and over 20% for fresh matter. With the increase of extract concentration, the value of the calculated palatability index decreases, and the deterrence index value increases.

13

Effect of fennel water extracts on reduction of feeding of pea leaf weevil

Biniaś B., Gospodarek J., Rusin M.

Journal of Ecological Engineering

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2016

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Vol. 17, nr 5

192--197

EN

The objective of the study was to examine the effect of aqueous extracts from fennel (Foeniculum vulgare Mill.) seeds at 2%, 5%, 10% and 20% concentrations on the feeding of peal leaf weevil (Sitona lineatus L.) on broad bean (Vicia faba L.). The experiment was conducted in the laboratory, in six replicates. Feeding intensity assessment was conducted by dipping leaves of broad bean in respective solutions of the extracts and determining the area of broad bean leaves, eaten by pea leaf weevil beetle in the 12 hour intervals. In addition, absolute deterrence index and palatability index were calculated. As a result of the observation no significant limiting effect of fennel seed aqueous extracts on the feeding of the pea leaf weevil females was shown. All of the used fennel extracts had inhibitory effect on the feeding of male S. linetaus and the strongest effect of extracts was observed in the first 36 hours of the experiment. The high values of the palatability index (particularly for the females) with relatively low absolute deterrence index, indicate limited possibilities of the use of aqueous extracts from fennel seeds for the protection against the feeding of the beetles from the genus Sitona.

14

The effect of water extracts from Artemisia absinthium L. on feeding of Leptinotarsa decemlineata Say. larvae

Rusin M., Gospodarek J., Biniaś B.

Journal of Research and Applications in Agricultural Engineering

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2015

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Vol. 60, nr 4

80--83

EN

The aim of this study was to determine the effect of water extracts from dry and fresh matter of Artemisia absinthium L. on feeding of Leptinotarsa decemlineata Say. L2 stage larvae. Dried extracts were prepared in concentrations of 2%, 5% and 10%, while the fresh plant in concentrations of 10%, 20% to 30%. Larvae feeding intensity assessment was carried out by dipping leaves of potato in respective solutions of the extracts, and then determining the weight of food eaten by larvae, and changes of larvae body weight once a day. In addition, absolute deterrence index and palatability index were calculated. The results of the experiment show that the extracts prepared from both dry and fresh matter at the highest concentrations (10% and 30% respectively) contributed to the greatest reduction of the larvae feeding and significant decreased their body weight. With increasing concentrations of extracts from both fresh and dry matter, their deterrent effect on the tested larvae was usually increasing while the value of palatability index was decreasing.

PL

Celem przeprowadzonych badań było określenie oddziaływania wodnych wyciągów z suchej i świeżej masy bylicy piołunu (Artemisia absinthium L.) na żerowanie larw L2 stonki ziemniaczanej (Leptinotarsa decemlineata Say.). Wyciągi przygotowano w stężeniach 2, 5 i 10% dla suchej masy oraz 10, 20 i 30% dla świeżej masy. Ocena intensywności żerowania larw została przeprowadzona poprzez moczenie liści ziemniaków w roztworach odpowiednich wyciągów, a następnie określenie masy pokarmu zjedzonego przez larwy oraz zmiany masy ich ciała, z częstotliwością raz na dobę. Dodatkowo obliczono bezwzględny wskaźnik deterentności i smakowitości. Na podstawie badań stwierdzono, że wyciągi sporządzone zarówno z suchej, jak i ze świeżej masy w najwyższych stężeniach przyczyniły się do największego ograniczenia żerowania larw oraz spowodowały istotny spadek masy ich ciała. Wraz ze wzrostem stężenia wyciągów z suchej oraz ze świeżej masy najczęściej zwiększało się także ich deterentne oddziaływanie w stosunku do analizowanego szkodnika, a także zmniejszała się wartość wskaźnika smakowitości.

15

Wykorzystanie grzyba owadobójczego Isaria fumosorosea do zwalczania szkodników w uprawie bobiku

Kuźniar T., Ropek D., Kulig B.

Proceedings of ECOpole

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2014

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Vol. 8, No. 1

201--207

PL

Celem badań było określenie skuteczności zastosowania zarodników grzyba owadobójczego Isaria fumosorosea (szczep AP 112) w ograniczaniu szkodliwości oprzędzika pręgowanego (Sitona lineatus L.) oraz strąkowca bobowego (Bruchus rufimanus Boh.) w uprawie bobiku. Doświadczenie poletkowe przeprowadzono w latach 2010-2011 w Prusach koło Krakowa. W doświadczeniu zastosowano dwie formy ochrony: zaprawianie nasion zarodnikami grzyba owadobójczego I. fumosorosea oraz opryskiwanie roślin zawiesiną zarodników w okresie wegetacyjnym. W obydwu formach ochrony zastosowano dawkę 1 · 1013 zarodników na ha. Zastosowana ochrona nie wpłynęła istotnie na procent liści uszkodzonych przez chrząszcze oprzędzika pręgowanego, natomiast wpłynęła na ograniczenie stopnia uszkodzeń brodawek korzeniowych bobiku, powodowanych przez larwy oprzędzika pręgowanego. Ponadto, w przypadku roślin chronionych, nasiona bobiku były w mniejszym stopniu uszkadzane przez strąkowca bobowego.

EN

The aim of this research is to evaluate the efficacy of the entomopathogenic fungus Isaria fumosorosea (AP 112 strain) in limiting the harm caused by the pea leaf weevil (Sitona lineatus L.) and the broad bean weevil (Bruchus rufimanus Boh.) in faba bean cultivation. Experiments in the field were conducted in 2010-2011 in Prusy, near Kraków. Two methods of application were used: seeds dressing with I. fumosorosea spores and spraying the plants with a spore suspension during the growing season. In both cases, I. fumosorosea was applied at a rate of 1 · 1013 spores · ha–1. The applied insecticide did not significantly influence the percentage of faba bean leaves damaged by the pea leaf weevil, but did reduce the damage to the plant’s radicular nipples of faba bean. Furthermore, faba bean seeds that were protected by I. fumosorosea were less damaged by the broad bean weevil.

16

Allelopathic activity of the norharmane-producing cyanobacterium Synechocystis aquatilis against cyanobacteria and microalgae

Mohamed Z.A.

Oceanological and Hydrobiological Studies

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2013

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Vol. 42, No. 1

1-7

EN

The cyanobacterium Synechocystis aquatilis was observed growing as a monospecies in enriched phytoplankton samples in the laboratory, indicating its allelopathic activity on coexisting phytoplankton species. Therefore, the present study screened the culture medium of an axenic strain of this cyanobacterium for the presence of allelechemicals with algicidal properties by thin-layer chromatography (TLC). The allelopathic effect of S. aquatilis was evaluated by co-cultivation of target species of toxic cyanobacteria and green algae with this cyanobacterium, as well as by evaluation of norharmane (β-carboline 9H-pyrido(3,4-b) indole) crude extract prepared from the culture medium of Synechocystis. The growth of target algal species was measured as a cell density after 6 days incubation. The results showed that S. aquatilis produced the indole alkaloid norharmane with large quantities in the culture medium (86 μg l-1). In co-cultivation experiments, S. aquatilis inhibited the growth of all tested cyanobacteria and green algae. Norharmane crude extract exhibited stronger inhibition of cyanobacteria (EC50 = 4.6-4.8 μg ml-1) than green algae (EC50= 6.3-6.4 μg ml-1) in a concentration-dependent manner, indicating its apparent role in the allelopathic activity of S. aquatilis. The possible applicability of the allelochemical, norharmane, as an algicide to prevent the formation of harmful algal bloom was discussed.

17

Survival of entomopathogenic nematodes from biopreparations in hen manure

Pezowicz E.

Chemia i Inżynieria Ekologiczna

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2004

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Vol. 11, nr 11

1297-1301

EN

Sensibility of invasive larvae of entomopathogenic nematodes used in biopreparations to control harmful pests to hen manure was tested under laboratory conditions. The study was aimed to select the least sensitive nematodes, which might be used to control populations of the lesser mealworm in poultry farms. Nematodes constituting four biopreparations: Entonem, Nemaplus, Owinema (based on S. feltiae) and Larvanem (based on H. bacteriophora) were tested. Three concentrations of 1%, 5% and 10% of hen manure were used with water as a control. Concentration of 10% of hen manure resulted in total mortality of all tested nematodes after 24 hours. Concentration of 1% showed low toxicity to nematodes and mortality was comparable with that in the controI. Concentration of 5% appeared to be least toxic to Owinema and Nemaplus.

PL

W warunkach laboratoryjnych zbadano wrażliwość larw inwazyjnych nicieni entomopatogenicznych wchodzących w skład biopreparatów służących do zwalczania szkodliwych owadów na odchody kurze. Celem badań było wytypowanie najmniej wrażliwych nicieni, które można by wykorzystać do redukcji liczebności populacji pleśniakowca lśniącego szkodnika występującego w fermach kurzych. Przetestowano nicienie wchodzące w skład czterech biopreparatów: Entonem, Nemaplus, Owinema z S. feltiae jako składnikiem aktywnym oraz Larvanem, którego składnikiem są nicienie H. megidis. Do badań wykorzystano trzy stężenia rekstraktu odchodów kurzych 1%, 5% oraz 10%, natomiast kontrolę stanowiła czysta woda. Stężenie 10% odchodów kurzych spowodowało całkowitą śmiertelność wszystkich badanych nicieni po 24 godzinach. Stężenie 1 % było stosunkowo mało toksyczne dla nicieni. Śmiertelność była porównywalna ze śmiertelnością w kontroli. Stężenie 5% najmniej toksyczne okazało dla biopreparatów Owinema i Nemaplus.