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1

Bioindication for Detecting Environmental Risks in Agrocenoses Contaminated with Heavy Metals

Lishchuk Alla, Parfenyk Alla, Horodyska Іnna, Boroday Vira, Khitrenko Tetiana, Tymoshenko Liudmyla

Journal of Ecological Engineering

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2023

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Vol. 24, nr 8

175--182

EN

The relevance and scientific and methodological approaches to the use of the bioindication method for assessing environmental risks in agrocenoses contaminated with toxic substances (in particular, heavy metals) were substantiated in the article. The study is based on the use of bee honey and heavy metal transfer coefficients in the sequential chain "soil – honey plant – bee honey". This ensures control over the content of toxicants in the soil and inflorescences of honey plants. Toxicant transfer coefficients can be used in the soil-plant-beekeeping products chain as indicators for developing environmental risk management tools. Bioindication is important for contaminated agricultural landscapes that are used as raw materials and forage lands for beekeeping and other environmental objects. The use of the bioindication method makes it possible to assess the environmental risks of toxic substance pollution of agroecosystems and natural biocenoses and to outline the boundaries of toxicant pollution of a certain area for the safe placement of apiaries and obtaining high-quality bee honey.

2

Analysis of the Impact of Stormwater Systems on Receiving Waters Based on the Analysis of Algal Community Structure

Kozłowska Aleksandra, Wojtaś Edyta, Piłat-Rożek Magdalena, Cybulski Michał, Łagód Grzegorz

Journal of Ecological Engineering

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2023

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Vol. 24, nr 9

139--147

EN

One of the important ways to prevent permanent environmental pollution is to constantly monitor its quality, which can be performed in several ways. The present bioindication study analyzed the level of diversity and abundance of biofilm microorganism communities, which illustrate the state of the studied aquatic environment, enabling to determine its quality. The impact of stormwater discharge on the receiver at particular points was evaluated on the basis of the reaction of selected microorganisms or their groups to the substances appearing in the watercourse. The study of indicator organisms gives information about the waters of a given body of water without expensive hydrochemical tests and without causing a burden on the environment during the production as well as disposal of reagents that are consumed in many classical physicochemical analyses. On the basis of selected algal species, the Shannon index and McArthur index were calculated, and the effect of storm sewer discharge on the communities of indicator organisms was determined. The best visible impact of storm sewer discharge was seen on the basis of the entire study cycle in relation to the median of the McArthur index.

3

Changes in the Structure of Soil Microscopic Fungi in the Territories of Yavoriv and Podorozhenie Sulfur Quarries

Oliferchuk Viktorija, Kendzora Nataliia, Hotsii Nataliya, Shukel Igor, Olejniuk-Puchniak Oksana, Samarska Mariia, Nagurskyy Oleg, Vasiichuk Viktor

Ecological Engineering & Environmental Technology

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2023

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Vol. 24, iss. 3

120-134

EN

The article presents the results of monitoring the soil mycobiota of sulfur quarries in LvivRegion. The dynamics of soil micromycete complexes as a result of their adaptation to the conditions of technozems, embryozems and zonal soils of sulfur quarries was studied. The increase in the number of colony-forming units (CFU) in the studied soils of the Yavoriv and Podorozhnie sulfur quarries over the years is specified, which indicates the active processes of decomposition of plant remains, the formation of stable processes of decomposition of cellulose and other complex compounds in the detrital block of the ecosystem. The change in the frequency of banding of selected species of micromycetes over the years was studied, depending on the content of SO4-2 in the soil solution. The functional structure of soil micromycete communities after sulfur extraction was established. A decrease in the frequency of occurrence of pathogens and toxin-producing agents and an increase in the frequency of occurrence of species that actively participate in the transformation of organic remains, and also act as plant endophytes and participants in mycorrhizal symbiosis were recorded. The species composition of micromycetes that grew in the presence of SO4-2 content in the soil solution, which exceeded the background level from 3.6% to 50%, was determined, the species-bioindicators of soil pollution with sulfur compounds were defined - Fusarium oxysporum, Paecilomyces lilacinum, P. waksmanii, P. nigricans, P. funiculosum, Trichoderma viride, Cladosporium cladosporioides, C. herbarum, Aureobasidium pullulans, Humicola grisea, Ulocladium consortiale, Alternaria alternate, Mortierella ramanniana var. angulispora. During 10 years of research a decrease in the content of SO4-2 in soils was noted, which is associated with an increase in the biodiversity of soil micromycetes and an enrichment of the phytocenotic cover.

4

Reaction of Aquatic Plants of Small Rivers of the Turkestan Region of Kazakhstan to Heavy Metal Ions

Issayeva Akmaral, Myrzabayeva Zhanar, Kidirbayeva K., Ibragimov Talgat, Baitasheva G., Tleukeyeva Assel

Journal of Ecological Engineering

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2022

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Vol. 23, nr 6

43--49

EN

Heavy metals are some of the environmental pollutants that have a serious impact on the environment. The analysis of hydromacrophytes growing in small rivers of the south of Kazakhstan with different contents of heavy metal salts revealed that the morphometric indicators of the same plant species differ significantly, depending on the level of total mineralization of the aquatic environment in different rivers of the Turkestan region. It has been established that two plant species can be used to bioindicate the content of lead ions in the aquatic environment: Azolla caroliniana Willd. and Veronica beccabunga L., which must be introduced into the tested aqueous solutions in the amount of 1.0 kg/m3 and 1.5–2.0 kg/m3, respectively, to fully cover the water column at different depths. The first morphological changes in plants, in the form of destruction of the structure of chloroplasts along the edges of unfolded leaves in A. caroliniana Willd. and slight withering of the lower underwater leaves in V. beccabunga L., occur already at a 1.5 mg/l Pb2+ content in water, and a further increase in the content of lead ions in water to 600.0–800.0 mg/l leads to the death of plants.

5

Usage of Worked Clay Absorbent as a Manifestation of an Ecologically Oriented Lifestyle

Palamarchuk Olga, Sakalova Halyna, Vasylinych Tamara, Lyubchak Lyudmila, Shevchuk Oksana, Matviichuk Oleksandr, Khodanaitska Olena

Ecological Engineering & Environmental Technology

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2022

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Vol. 23, iss. 2

90--96

EN

The article deals with the development of new eco-innovative technologies, evaluation of their effectiveness and raising the widest possible awareness of those categories to the people who could effectively use the proposed technologies. It was found that the personal position of economic entities is the core of the structural organization of psychological readiness for ecologically oriented innovation and is manifested in the forms of free ecologically oriented will. The paper discusses the main methods of removal of nutrients from wastewater, as well as presents promising technologies and techniques. It contains the recommendations for optimal conditions for the precipitation of ammonia nitrogen from an ion exchange regenerator for further use as an ammonium fertilizer. The maximum efficiency of ammonium nitrogen removal was established. According to the results of the best options, the deposition of real ion exchange concentrates was carried out. The humidity level was set during the drying of mineral fertilizers. The moisture content determined the content of sediment, which is close to the number of water molecules to the formula of the fertilizer MgNH4PO4 · 5H2O. Complex phosphate fertilizer struvite was characterized. A comparative agronomic evaluation of synthetic struvite was performed. The influence of struvite on the processes of germination of cultivated plants was carried out. The results show that the method of obtaining struvite does not significantly affect the processes of wheat germination, and the determining factor in the effectiveness of fertilizers is the method of its application and dosage.

6

The Use of the Triad Approach to Ecological Assessment of the State of a Reservoir Receiving Wastewater From a Thermal Power Plant

Mahanova Elena

Journal of Ecological Engineering

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2021

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Vol. 22, nr 2

155--160

EN

Thermal power plants (TPP) belong to the enterprises that accompany the development of any city. These industrial facilities consume and discharge large amounts of water. Therefore, thermal power plants are often located near water bodies that need to assess their ecological state and predict the consequences of the anthropogenic impact. The purpose of the work was to assess the ecological state of the floodplain Lake Ivanovskoye (Russia, Kirov region), which receives the wastewater from a thermal power plant, using a triadic approach and comparing the results of chemical analysis, bioassay and bioindication. It was shown that the main pollutant in the lake and the canal connecting Lake Ivanovskoye with the nearest river Vyatka is ammonium ion (up to 3.2 mg/dm3). In addition, the Russian standards for biological oxygen consumption are exceeded (analysis period is 20 days). The tests for the toxicity of wastewater showed inhibition of Paramecium caudatum and Escherichia coli reactions. In contrast, natural surface water from Lake Ivanovskoye stimulated the test functions of Scenedesmus quadricauda, P. caudatum, and E. coli. The death of Daphnia magna in the samples were not recorded. The totality of the facts pointed to the organic nature of pollution, leading to the anthropogenic eutrophication of the lake. The hypothesis was tested by using the bioindication method and by analyzing the totality of species of coastal aquatic plants. It was shown that the reservoir belongs to the mesotrophic type with a moderate degree of pollution, which decreases with distance from the wastewater discharge points. The increase in the trophicity of the reservoir is facilitated by thermal pollution (up to +15℃) and an alkaline pH of wastewater (up to 9.2). Thus, the joint analysis of the results of chemical analysis, bioassay and bioindication made it possible to carry out an objective assessment of the ecological state of the reservoir and identify the reasons for the revealed facts.

7

Monitoring of the Influence of Landfills on the Atmospheric Air Using Bioindication Methods on the Example of the Zhytomyr Landfill, Ukraine

Malovanyy Myroslav, Korbut Mariia, Davydova Irina, Tymchuk Ivan

Journal of Ecological Engineering

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2021

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Vol. 22, nr 6

36--49

EN

The paper presents the results of research on the implementation of monitoring of the landfills influence on the atmospheric air using bioindication methods on the example of the Zhytomyr landfill (Ukraine)–one of the typical Ukrainian landfills, which is operated with minimal environmental protection measures. A comprehensive study of atmospheric air pollution in the landfill area was carried out using a human sensor system and an integral assessment using bioindication (lichens were chosen as bioindicators). The study revealed a reliable exponential dependence for modeling the relationship between the distance from the source of gas formation (solid waste landfill in the city of Zhytomyr) and the intensity of odor. The level of atmospheric air pollution was also determined based on the assessment of the projective coverage of the tree trunk with lichens. The research revealed an exponential dependence for modeling the relationship between the distance from the landfill boundary and the total coverage of the tree trunk with lichens. The results of the analysis confirmed the correctness of the use of the aforementioned method. The relationship between the results obtained by the lichen indication method and the strength of the odor from the landfill was evaluated. Based on the results of calculating the relative values of the assessment of atmospheric air pollution by the method of coverage of the tree trunk with lichens and the strength of the odor, a regression analysis was carried out and mathematical relationships were determined, that most accurately describe the change in these indicators with distance from the landfill boundary. It is proved that both tested methods can be used in Ukrainian realities to monitor the state of atmospheric air in the area of influence of municipal landfills, depending on the purpose of research. The analysis of the research results confirmed the significance of the results of the obtained assessment of the impact of the Zhytomyr solid waste landfill on the state of atmospheric air. The materials of these studies can find further application for the formation of databases of knowledge of landfills, which will allow to develop a strategy for managing them as an object of increased environmental hazard.

8

Sewage Sludge as a Component to Create a Substrate for Biological Reclamation

Tymchuk Ivan, Malovanyy Myroslav, Shkvirko Oksana, Yatsukh Katerina

Ecological Engineering & Environmental Technology

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2021

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Vol. 22, iss. 4

101--110

EN

The main ways of sewage sludge disposal in the European Union and Ukraine were considered. The main problems of sludge utilization in Ukraine were identified. Research on the possibility of using a substrate based on sewage sludge for biological reclamation of disturbed lands was conducted. The quality of the sludge from Lviv WWTP was determined. The best substrate composition for biological land reclamation was determined by using bioindication. It was established that the use of settled sludge observed inhibition of plant growth and development. Introducing additional components to the substrate, in the form of sorbents, makes it possible to increase the content of sewage sludge. Positive changes in plant growth and development when using a soil-based substrate, sewage sludge and sorbent were observed.

9

Assessment of the Condition of Pine Plantations in the Area of Influence of Municipal Waste Landfills on the Example of the Zhytomyr Landfill, Ukraine

Korbut Mariia, Malovanyy Myroslav, Davydova Irina, Grechanik Ruslan, Tymchuk Ivan, Popovych Olena

Ecological Engineering & Environmental Technology

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2021

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Vol. 22, iss. 5

40--46

EN

The paper presents the results of research to assess the degree of transformation of forest ecosystems in the area of influence of the Zhytomyr landfill (Ukraine) - one of the typical Ukrainian landfills, which is operated with minimal implementation of environmental protection measures. The study was conducted to identify the relationship between the condition of plantations and the functioning of municipal solid waste landfills. In the study of the estimation of the transformation degree of forest ecosystems in the area of influence of the municipal solid waste landfill in Zhytomyr, it was established that in the studied areas there was a decrease in bonitet by I-II classes, completeness by 0.1-0.2 units, growth by 13-35% in comparison with background values. On the basis of the research of the condition of pine plantations by categories of vital activity of trees the integral indicator was calculated – the index of the condition of forest stands, which characterizes the degree of their damage in the event of aerial pollution of soil and groundwater. It was determined that the maximum values of the index of forest stands condition were observed in the area closest to the landfill (2.88 units), i.e, these are severely weakened stands, the minimum in the control (1.28). Based on the results of the studies, it was established that Scots pine is a convenient and acceptable bioindicator for assessing the state of the environment in the area of influence of municipal landfills provided that its distribution is uniform on the territory and it is available for research. The sanitary condition of pine plantations deteriorates with the approach to the source of pollution. According to the established indices of the condition of pine plantations, a regression analysis was performed and a mathematical dependence was determined, which most accurately describes the change of these indicators with the distance from the landfill boundary. The approach used for bioindication studies of conifers can be recommended for implementation in practice as a method for assessing the transformation of the environment in the area of influence of municipal solid waste landfills.

10

Heavy metals accumulation in silver fir needles in Swietokrzyski National Park

Kozłowski Rafał, Strzyż Małgorzata

Chemistry-Didactics-Ecology-Metrology

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2021

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R. 26, NR 1-2

65--72

EN

The article demonstrates the results of the research studies related to the air polluted with heavy metals in the area of Swietokrzyski National Park. The two-year-old needles of Abies alba (Mill) were used as the bioindicator with the intention of carrying out the research. The studies were conducted in the autumn of 2012. The results showed the spatial variability of concentrations in the range of the analysed metal deposition patterns in the needles of Abies alba (Mill). The average content of the analysed elements seemed to be the highest in the case of zinc (26.6 mg·kg–1 d.m.), strontium (6.5 mg·kg–1 d.m.) and nickel (1.6 mg·kg–1 d.m.). The research studies revealed that the significant role in determining the content of heavy metals in the two-year-old needles was played by the communication. The highest values were recorded at the research sites situated in the immediate neighbourhood of the voivodeship roads. It was also confirmed that the content of metals was influenced by the so-called low emission from the household and welfare sector together with the remote imission.

11

Diversity and ecological characteristic of algae and cyanobacteria of thermokarst lakes in Yakutia (northeastern Russia)

Kopyrina Liubov, Pshennikova Elena, Barinova Sophia

Oceanological and Hydrobiological Studies

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2020

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Vol. 49, No. 2

99--122

EN

KopyrinaA total of 437 species (453 with infraspecific taxa) of algae and Cyanobacteria were identified in 2034 samples collected during the first research on nine thermokarst lakes in Yakutia carried out in 1986–1994 and 2001–2015. The richest algal flora was determined in Lake Aalah, represented by 241 taxa. Bacillariophyta species dominated in four lakes, whereas Chlorophyta and Cyanobacteria dominated in the others. The algal bioindication demonstrated preferences for benthic and plankticbenthic life, temperate temperature, low alkalinity, waters with moderate oxygenation, low to medium enrichment with chloride, low organic pollution, and mesotrophic state. Comparative floristic, CANOCO and JASP statistical methods divided the species and environmental variables into groups of lakes related to landscape terraces. Local landscape positions and high summer water temperatures may play a major role in the formation of algal flora. The index of algae taxa per area stabilized at about 70 species per km2. The highest polymorphism of algae floras, defined by the Subspecies/Species Index, was determined for the lakes of the Tungulunskaya terrace: Lake Ynakh, Lake Nal Tungulu and Lake Tungulu (1.041–1.058), and the average total flora was 1.036. Our results can help in monitoring and protecting the ecosystems of thermokarst lakes in Yakutia in the harsh climate environment of Northeastern Eurasia.

12

Evaluation of the hydro-ecological quality of the aquatic habitat of the Váh River

Štefunková Zuzana, Macura Viliam, Doláková Gréta, Majorošová Martina

Journal of Water and Land Development

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2020

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no. 46

209--215

EN

There is a cascade of hydroelectric power plants built on the Váh River. From a water-management point of view, the natural channel is used to drain extreme discharges. During most of the year, discharges are regulated by water-management structures. These discharges are not used for energy-related purposes; therefore, it is important to determine the optimal discharge that will not negatively affect the ecosystem of the stream. The minimum balance discharge (hydro-ecological discharge) was determined based on the instream flow incremental methodology (IFIM) using the riverine habitat simulation system (RHABSIM). Input data were obtained from direct measurements on three reference reaches in the area between the cities Piešťany and Nové Mesto nad Váhom. Hydraulic flow characteristics were derived from three measurements at different water levels. Habitat quality was represented by ichthyofauna. Data to determine the habitat suit-ability curves of fish were obtained using a diving technique to collect video footage. The modelling resulted in the quantification of the effect of discharge on ichthyofauna as a bio-indicator of habitat quality, which implied the need of 20 m3∙s–1 for a minimum balance discharge in summer.

13

Wastewater a Source of Nutrients for Crops Growth and Development

Tymchuk Ivan, Shkvirko Oksana, Sakalova Halyna, Malovanyy Myroslav, Dabizhuk Tetiana, Shevchuk Oksana, Matviichuk Oleksandr, Vasylinych Tamara

Journal of Ecological Engineering

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2020

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Vol. 21, nr 5

88--96

EN

The article deals with the main methods of nutrient removal from wastewater, as well as presents promising technologies and techniques. Struvite was characterized by a complex phosphorus fertilizer. The influence of struvite on the processes of germination of cultivated plants was investigated. The possibility of creating a growth substrate as an inexpensive and effective alternative to the disposal of the man-made wastes that contain an organic component was identified. The composition of the growth substrate was selected to ensure its functional properties. The growth substrate was tested by bioindication. Subsequently, the modification of the substrate by adding natural sorbents.

14

Evaluation of Stormwater System Influence on the River Using Algae

Babko Roman, Szulżyk-Cieplak Joanna, Danko Yaroslav, Duda Sylwia, Kirichenko-Babko Marina, Łagód Grzegorz

Journal of Ecological Engineering

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2020

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Vol. 21, nr 2

214--221

EN

One of the widespread sources of river pollution is the wastewater coming from both wastewater treatment plants and the stormwater system. Wastewater can vary significantly in composition and concentration of substances introduced into water bodies. Municipal effluents may contain significant amounts of organic matter and ammonia. Storm drains are diverse in composition and depend on the nature of the surface from which the water collects, but carry more suspended solids and less nutrients. The research was aimed at assessing the effect of surface runoff collected by the stormwater system from the territory of the city of Lublin on the Bystrica River using popular environmental indices, calculated on the basis of periphytonic algae species abundances: species number, Shannon’s H, rarefied species number, Pielou’s evenness, trophic diatom index (TDI). It was observed that the correspondence between a species diversity and the quality of the environment is not always straightforward. Therefore, the periphytonic algae diversity increases under the influence of runoff, as evidenced by the Shannon index. Nevertheless, pronounced changes are noted in the structure of the algal community, as shown by the Pielou index and NMDS. However, these changes in the structure are invisible if the trophic diatom index (TDI) is relied upon.

15

Heavy metals acccumulation in lichens in Swietokrzyski National Park

Kozłowski Rafał, Strzyż Małgorzata

Ecological Chemistry and Engineering. A

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2020

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Vol. 27, nr 1-2

1--8

EN

The article reveals the outcomes of the research studies related to air polluted with heavy metals in Swietokrzyski National Park. The thalli of Hypogymnia physodes L. were used as the bioindicator in order to conduct the research. The samples of monk’s-hood lichen were transplanted from the unpolluted area of north-eastern Poland to the area of Swietokrzyski National Park. The research studies were carried out in the warmer half of 2019. The received outcomes indicated the spatial variability of concentrations in the range of analysed metal deposition patterns in the lichen thalli. The average content of the analysed elements tended to be the highest in the case of iron (1,111 mg × kg–1 d.m.), zinc (87.7 mg × kg–1 d.m.) and strontium (27.7 mg × kg–1 d.m.). The research revealed that the important role in determining the content of heavy metals in the lichen thalli was played by the communication. The highest values were recorded at the sites located in the immediate neighbourhood of the voivodeship roads. It was verified that the content of metals was also influenced by the so-called low emission from the household and welfare sector as well as remote immission.

16

Occurrence of Oribatid Mites During the Revitalization of Bare Root Forest Nursery

Klimek Andrzej, Rolbiecki Stanisław, Rolbiecki Roman, Stachowski Piotr, Podsiadło Cezary

Rocznik Ochrona Środowiska

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2019

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Tom 21, cz. 2

1533--1550

EN

The study was conducted in the years 2008-2010 in the bare root nursery Białe Błota that produced pine, beech and linden, and belonged to Bydgoszcz Forest District. The experiments included the following variants: C – control, O – organic fertilization, M – mulching with raw humus, OM – organic fertilization and mulching with raw humus. Relatively small but significant increase in the number and diversity of Oribatida species was observed in O variant for the deciduous trees. Mulching was the soil revitalization method that clearly improved the density and biodiversity of Oribatida species. In total, the investigated area harbored 37 species of oribatid mites. The most effective bioindicator and stimulant of soil revitalization was a common fungivorous species Tectocepheus velatus.

PL

Badania były prowadzone w latach 2008-2010 w należącej do Nadleśnictwa Bydgoszcz, szkółce leśnej Białe Błota produkującej sosnę, buk oraz lipę. Badania prowadzone były w następujących wariantach: C – kontrola, O – nawożenie organiczne, M – mulczowanie świeżym humusem, OM – nawożenie organiczne i mulczowanie świeżym humusem. Stosunkowo mały, ale znaczący wzrost liczby i zróżnicowania gatunkowego mechowców zaobserwowano w wariancie O dla drzew liściastych. Mulczowanie było metodą rewitalizacji gleb, która wyraźnie wpłynęła na intensywność i zróżnicowanie gatunkowe mechowców. Na badanej powierzchni stwierdzono ogółem 37 gatunków mechowców. Najskuteczniejszym bioindykatorem i stymulatorem rewitalizacji gleby był powszechnie występujący gatunek grzybożerny Tectocepheus velatus.

17

Microphytobenthos as an indicator of water quality and organic pollution in the western coastal zone of the Sea of Azov

Barinova Sophia, Bondarenko Anna, Ryabushko Larisa, Kapranov Sergey

Oceanological and Hydrobiological Studies

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2019

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Vol. 48, No. 2

125--139

EN

Bioindication of organic pollution and seawater quality was carried out for the first time in the western part of the Sea of Azov on the basis of species composition and quantitative characteristics of microphytobenthos. A total of 229 algal samples were collected at 17 sites over the period of 2005-2014 on three different substrates in three areas under study: Sivash Gulf, the Kerch Strait and Cape Kazantip. In total, 200 taxa of algae were found, which belong to six taxonomic divisions with a predominance of diatoms. Among those, 108 taxa are indicators of substrate, water temperature, salinity, water pH, trophic state, the type of nutrition and organic pollution of water. It has been shown that the most active self-purification of water takes place in communities on stony substrates. The largest number of algae species (50%) occurs in the cleanest waters of the Kazantip Nature Reserve (Water Quality Classes I–II). The species composition of organic pollution indicators in Sivash Gulf corresponds to waters of Classes III–IV, which are more polluted than those of the Kerch Strait and Cape Kazantip. All the studied areas of the Sea of Azov are cleaner compared to some waters of the Eastern Mediterranean and the Sea of Japan.

18

Effect of Stormwater System on the Receiver

Babko Roman, Szulżyk-Cieplak Joanna, Danko Yaroslav, Duda Sylwia, Kirichenko-Babko Marina, Łagód Grzegorz

Journal of Ecological Engineering

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2019

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Vol. 20, nr 6

52--59

EN

The surface water quality assessment could be based on a combined physical and chemical analysis, but it could also be determined with bioindication methods. Classical physicochemical analysis is in most cases more expensive and time-consuming than the bioindication methods. This type of analysis also requires expensive equipment and shows the situation in the water only at the moment of sampling. Although the bioindication methods are often complicated, they allow a relatively inexpensive estimation of the water quality. Moreover, during their implementation, the substances harmful to the environment are not generated, and the obtained results usually reflect the total interaction of all factors and substances to the analyzed living organisms. Indicator organisms or their communities applied to the research, with identified ranges of tolerance to selected factors, could help to determine the physical and chemical parameters of water. This paper presents a bioindication study with an effect of stormwater system on the receiver – the Bystrzyca river, in Lublin, Poland. The level of saprophyty of the river sector was calculated based on the selected species of algae (diatoms and green algae) and the influence of the stormwater discharge on the communities of these organisms was determined.

19

Wykorzystanie baterii biotestów do oceny wpływu zrzutów przemysłowych na ekosystem wodny rzeki Kłodnicy

Zgórska Aleksandra, Bondaruk Jan, Dudziak Mariusz

Inżynieria Ekologiczna

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2019

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Vol. 20, nr 2

15--23

PL

W celu oszacowania wpływu zanieczyszczeń antropogenicznych na ekosystemy wodne, dla rzeczywistych próbek środowiskowych przeprowadzono analizę ekotoksykologiczną. Badania bioindykacyjne wykonano dla próbek wód powierzchniowych pobranych w 10 wyznaczonych miejscach pomiarowych rz. Kłodnicy na odcinku od źródła – do ujścia Potoku Bielszowickiego. W ramach analizy ekotoksykologicznej zastosowano baterię biotestów, obejmującą testy na skorupiakach (Daphnia magna), bakteriach (Vibrio fiascherii) oraz roślinach hydrofitowych (Lemna minor). Z przeprowadzonych badań wynika, że za wyjątkiem miejsc zlokalizowanych bezpośrednio za punktami zrzutu, próbki wód rzecznych nie wpływają negatywnie na organizmy testowe. Żadna z przeanalizowanych próbek wody nie wpłynęła na przeżywalność skorupiaków z gatunku Daphnia magna. Podobne wyniki uzyskano w teście MICROTOX® z wykorzystaniem bakterii bioluminescencyjnych Vibrio fischerii, w którym, w skutek stymulacji procesów metabolicznych u organizmów testowych odnotowano wystąpienie zjawiska hormezy. Osiem z dziesięciu przeanalizowanych próbek wody sklasyfikowano jako próbki nietoksyczne (Klasa I – brak ostrego zagrożenia). Z uwagi na wynik testu fitotoksyczności (inhibicja na poziomie 25% i 50%) dwie z dziesięciu przeanalizowanych próbek wód powierzchniowych sklasyfikowano jako próbki charakteryzujące się niewielkim ostrym zagrożeniem (Klasa II).

EN

The main goal of the research was the assessment of the impact of industrial and municipal wastewater discharges on aquatic ecosystems of Kłodnica river. The conducted studies were based on bioindication methods. Within the ecotoxicological analyses the biotest battery was used. The scope of the analysis included determination of influence of anthropogenic pollutants present in Kłodnica river on growth of hydroponic plant (Lemna minor). Also the acute toxicity test using crustacean (Daphnia magna) and bioluminescence bacteria (Vibrio fischeri) were performed. Toxic effects were observed only during the phytotoxicity test in which the plant growth inhibition level exceed respectively 25% and 50%. During the research no other toxic effects of analysed river water samples was observed. The analysed samples did not affect the inhibition of metabolic process of bacteria Vibrio fischerii, on the contrary, the water high salinity stimulate it. During the MICROTOX® test the phenomenon of hormesis was observed.

20

Klasyfikacja toksyczności ścieków szpitalnych w odniesieniu do kryteriów ich szkodliwości względem biocenoz wodnych

Zgórska Aleksandra, Grabińska-Sota Elżbieta

Inżynieria Ekologiczna

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2019

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Vol. 20, nr 4

5--13

PL

W celu określenia potencjału toksykologicznego, a tym samym prognozowania skutków jakie może wywołać depozycja nieoczyszczonych lub też niedostatecznie oczyszczonych ścieków szpitalnych względem biocenoz zasiedlających akweny wodne stanowiące odbiorniki ścieków po procesie oczyszczania, przeprowadzono analizę ekotoksykologiczną. Badania przeprowadzono na rzeczywistych próbkach ścieków szpitalnych. Zakres analizy ekotoksykologicznej obejmował przeprowadzenie następujących testów: testy immobilizacji skorupiaków Daphnia magna, test przeżywalności skorupiaków z gatunku Thamnocephalus platyourus oraz Artemia salina, a także test inhibicji wzrostu kolonii orzęsków Tetrahymena termophila. W ramach każdego testu, w oparciu o wyniki cząstkowe wyznaczono wartości wskaźników ekotoksykologicznych EC 50 (ang. effect concentration), które następnie przekształcono na jednostki toksyczności TU (ang. Toxic Unit) na podstawie których dokonano klasyfikacji toksyczności analizowanych próbek ścieków. W ramach przeprowadzonych badań, próbki ścieków szpitalnych sklasyfikowane zostały jako medium charakteryzującego się ostrą toksycznością. Z przeprowadzonych badań wynika, że ścieki szpitalne stanowią poważne zagrożenie względem organizmów zasiedlających ekosystemy wodne, a ich niedostateczne oczyszczenie lub też niekontrolowany zrzut do środowiska może wywołać katastrofalne skutki względem organizmów zasiedlających narażoną biocenozę.

EN

In order to assess the toxicological potential and thus forecast the potential effect posed by untreated hospital wastewater discharges according to exposed ecosystem, the ecotoxicological analysis were performed. During the study the real samples of hospital wastewater were used. The ecotoxicological analysis included the following tests: immobilization test according to crustacean Daphnia magna; mortality test according to crustacean Thamnocephalus platyourus and Artemia salina and growth inhibition test relative to ciliates Tetrahymena termophila. Within each test, the value of ecotoxicological indicators EC50 (effect concentration) were estimated. The EC50 value were transformed into the Toxic Unit (TU), basis of which the analyzed hospital wastewater samples were classified. The results proves that hospital wastewater pose a serious threat to exposed aquatic organisms and their insufficient purification as well as uncontrolled discharge into the environment may cause adverse changes in the environment.