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Simultaneous estimation of anti-cancer terpenoids in pharmaceutical nanoformulation by RP-HPLC and HPTLC

Parveen R., Ahmad F. J., Iqbal Z., Singh M., Kamal Y., Ahmad S.

Acta Chromatographica

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2014

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Vol. 26, no. 2

391--400

EN

Andrographolide and betulinic acid are the terpenoids having potential anti-cancer activity. The cytotoxicity activity of both the drugs was carried out separately and in combination on liver cancer HepG2 cell lines. High-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods were developed and validated for simultaneous estimation of these two terpenoids as per the International Conference on Harmonization (ICH) guidelines, which was applied for quantification in nanoformulation. The retention time by HPLC and retardation factor by HPTLC for andrographolide and betulinic acid were found to be 2.2 and 6.6 min, and 0.24 ± 0.01 and 0.66 ± 0.01, respectively. Both the methods were validated for accuracy, precision, repeatability, robustness, limit of detection (LOD), and limit of quantitation (LOQ). The content of andrographolide and betulinic acid in nanoformulation was found to be 96.0% and 98.0% by HPLC and 96.59% and 98.33% by HPTLC, respectively, of labelled claim.

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Wpływ fosfatydylobetulin na bakterie Staphylococcus aureus

Malicki A., Tubek-Barycza B., Janik P., Szpak M., Źródłowska-Danek J., Grabowski T., Szyplik K., Wawrzeńczyk C.

Przemysł Chemiczny

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2013

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T. 92, nr 8

1532-1537

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Synteza i aktywność cytotoksyczna estrów betuliny i kwasu betulinowego z kwasami terpenowymi

Barycza B., Pawlak A., Poradowski D., Obmińska-Mrukowicz B., Wawrzeńczyk C.

Przemysł Chemiczny

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2013

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T. 92, nr 5

806-812

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Alkohol betulinowy i jego pochodne

Tomaszkiewicz-Potępa A., Piękoś M.

Wiadomości Chemiczne

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2002

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[Z] 56, 11-12

983-1001

EN

Betulinic alkohol (lup-20(29)-en-3,28-diol) is a naturally occuring triterpene compound forming the principal extractive substance of the birch bark. The content of betulinol in the outer bark varies between 10-30% depending on the growth condition, age, season etc. Chemically, betulinol is a pentacyclic triterpene alcohol belonging to a lupane series of compouds. The characteristic feature of the lupane group is the five membered ring E and a-isopropyl group at C-19. Suberin and triterpenoids, the two major component groups in the outer bark of birch, have been investigated by many workers. Analytical procedures, starting with sample extraction and hydrolysis with ethanolic alkali, were developed for routine analysis, which triterpenoids to be identified by the chromatographic and spectroscopic methods. The functional groups in the betulinol molecule comprise primary and secondary hydroxyl groups and a double bond. Methods of synthesis and use of the chemical modification of betulinol has been investigated by many workers. The most investigated products from betulinol now are betulinic acid and his derivatives. It was isolated for the first time from many of plants. Betulin was convert to betulinic acid using two different synthetic routes. The first approach involved an oxidation of betulin using Jones reagent to betulonic acid and subsequent NaBH4 reduction to betulinic acid. The second approach involved steps utilizing different protecting groups on the alcohol functional groups of betulin and Jones’ oxidation to circumvent the isomerization of the secondary alcohol of betulinic acid. Betulinic acid was identified as a highly selective inhibitor of human melanoma. It has recently been reported to possess antitumor activity against cultured human melanoma cells. Betulinic acid has been modified at C-3, C-20 and C-28 positions. A series of w-undecanoic amides of betulinic acid derivatives were synthesized and evaluated for activity in human immunodeficiency virus type 1 (HIV-1). A novel series of w - aminoalkanoic acid derivatives of betulinic acid were synthesized and evaluated for their activity against human immunodeficiency virus (HIV). Effect of betulinic acid and radiotherapy on survival was demonstrated by autors.