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1 2009

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Comparative analysis of the chromatographic fingerprints of twenty different sage ( Salvia L.) species

Daszykowski M., Sajewicz M., Rzepa J., Hajnos M., Staszek D, Wojtal Ł., Kowalska T., Waksmundzka-Hajnos M., Walczak B.

Acta Chromatographica

2009

Vol. 21, no. 4

513--530

Comparative analysis of twenty different sage ( Salvia L.) species grown in Poland has been performed on the basis of two types of chromatographic fingerprints. For efficient preprocessing and comparison of these fingerprints, chemometric methods were used. The main emphasis was on preprocessing of herbal fingerprints and selecting a suitable preprocessing strategy for exploring differences among them. After successful preprocessing of the fingerprints, principal component analysis was used to reveal chemical differences among the samples. An outcome of the comparative analysis was to pinpoint specific regions of the fingerprints indicative of differences among the samples. In fingerprints of the volatile fraction from the sage ( Salvia L.) species, obtained from head-space gas chromatography coupled with mass spectrometry, important regions were identified and associated with the presence of camphene, limonene, and eucalyptol in these samples.