Wyniki wyszukiwania - BazTech

1

Engineered atenolol-glycoconjugates to target H9c2 cardiomyocyte cell lines

Kumbha Smita Tukaram, Bhatia Manish Sudesh, Patil Shitalkumar Shivgonda

Acta Innovations

|

2022

|

no. 43

36--43

EN

Background: One of the most important fields of biomedical engineering study nowadays is targeted drug delivery to specific cells. A drug's therapeutic efficacy can be improved and optimised by tightly targeting it to a pathophysiologically essential tissue architecture. The goal of this research is to develop saccharide conjugates for the targeted delivery of Atenolol, a -blocker. Methods: Galactose (monosaccharide), pectin (polysaccharide), and chitosan were chosen as the saccharides (polysaccharide). By grafting Atenolol with the modified saccharides, the conjugates were created. Spectroscopic and thermal studies were used to describe the chemically changed saccharides conjugates. H9c2 cell lines were used to conduct drug release research and cellular uptake studies. To investigate cytotoxicity, a brine shrimp lethality test was done. Results: The outcomes exhibit that Atenolol-modified saccharide conjugates can productively convey the medication to the target. Conclusion: It can be inferred that the improvement of saccharide-drug conjugates can be a compelling methodology for targeting cardiovascular medication.

2

Eco-Friendly Simultaneous Chromatographic Determination of Amiloride Hydrochloride, Atenolol, and Hydrochlorothiazide in Urine

Albishri H. M., El-Hady D., Tayeb R. A.

Acta Chromatographica

|

2015

|

Vol. 27, no. 3

461--476

EN

Pharmaceutical industry concerned recently with eco-friendly analytical methods to reduce the environmental pollution. The using of toxic organic solvents for the analysis of drugs is critical. In the current work, several simple and less costly approaches such as micellar and/or cyclodextrin liquid chromatography were discussed. A new eco-friendly and simple chromatographic analysis of the ternary mixture of amiloride hydrochloride (AM), atenolol (AT), and hydrochlorothiazide (HZ) in urine by hydroxypropyl-beta-cyclodextrin (HP-β-CD) bonded stationary phase was investigated. The experimental conditions were optimized and validated based on International Conference on Harmonization (ICH) Q2R1 guidelines to detect analytes by isocratic mobile phase of phosphate buffer (5.0 mmol L-1, pH 7.0) in the presence of 0.5 mL min-1 flow rate, 25.0 °C, and 280 nm. Linearity, accuracy, and precision were found to be acceptable over the concentration range of 0.05–20.0 μg Ml-1 for AM, 0.05–50.0 μg Ml-1 for AT and 0.05–50.0 μg Ml-1 for HZ. The proposed method was precise, selective, and sensitive enough for the routine analysis of ternary mixture at therapeutic urine levels. The inclusion complexation and the appendant hydroxyl groups of HP-β-CD were considered the main reasons for assisting in adequate separation of the drugs. On the other hand, the presence of kosmotropic phosphate ions could solubilize the protein and could strengthen the selective inclusion of drugs inside HP-β-CD cavity. Urinary excretion studies showed that the detection of drugs is possible up to 24 h after their ingestion.

3

Simultaneous RP-HPLC analysis of atenolol, hydrochlorothiazide, and losartan potassium in a tablet formulation

Thomas A. B, Chavan U. B, Nanda R. K, Kothapalli L. P, Jagdale S. N, Dighe S. B, Deshpande A. D

Acta Chromatographica

|

2010

|

Vol. 22, no. 2

219--226

EN

Simultaneous analysis of atenolol (Atn), hydrochlorothiazide (Hctz) and losartan potassium (Los) in solid dosage forms has been achieved by reversed-phase high-performance liquid chromatography on a C 18 column with a 0.035 M potassium dihydrogen orthophosphate-acetonitrile gradient as mobile phase and UV detection at 225 nm. The retention times for Atn, Hctz, and Los were 2.91, 4.75, and 7.52 min, respectively, with mean recoveries of 99.67, 99.89, and 100.69%. The method was validated in accordance with ICH guidelines. Because of its simplicity and high precision and accuracy, the method can be used for analysis of atenolol, hydrochlorothiazide and losartan potassium in pharmaceutical preparations.

4

Simultaneous TLC-densitometric analysis of atenolol and lercanidipine hydrochloride in tablets

Deore P. V., Shirkhedkar A. A., Surana S. J.

Acta Chromatographica

|

2008

|

Vol. 20, no. 3

463-473

EN

A new simple, precise, accurate, and selective thin-layer chromatographic (TLC) method has been developed for simultaneous analysis of atenolol and lercanidipine hydrochloride in a tablet dosage form. Chromatographic separation was achieved on aluminum foil plates precoated with silica gel 60F 254 , with toluene-methanoltriethylamine 3.5:1.5:0.1 (v/v) as mobile phase. Detection was performed densitometrically at 275 nm. The R F of atenolol and lercanidipine hydrochloride were 0.24 and 0.68, respectively. The reliability of the method was assessed by evaluation of linearity (2000-12000 ng per band for atenolol and 400-2400 ng per band for lercanidipine hydrochloride), accuracy (98.94 š 0.30% for atenolol and 99.75 š 0.69% for lercanidipine hydrochloride), and specificity, in accordance with ICH guidelines. The method can be used for routine simultaneous analysis of atenolol and lercanidipine hydrochloride in pharmaceutical formulations.

5

Artificial neural network calibration models for simultaneous spectrophotometric determination of atenolol and losartan potassium in tablets

Satyanarayana D., Kannan K., Manavalan R.

Chemia Analityczna

|

2006

|

Vol. 51, No. 5

771-784

EN

Simultaneous determination of all drug components in multicomponent pharmaceutical dosage form has been performed applying UV spectra photometry and calibration models based on artificial neural networks. The proposed approach is a simple alternative to using separate models for each component. A novel approach for calibration using computed spectral dataset derived from three spectra of each component has been described. Spectra of Atenolol and Losartan potassium were recorded in the wavelength range 2! 5-275 nm, interval 1 nm, at several concentrations of both analytcs within their linear calibration range and were subsequently used to compute the composition of the calibration mixture. Neural networks trained by Levenberg-Marquardt algorithm were used for building and optimizing calibration models utilizing MATLAB® Neural Network Toolbox. Two types of neural network models were compared to the principal component regression model. Calibration model was thoroughly evaluated at several concentration levels using the spectra obtained for 76 synthetic binary mixtures prepared using orthogona-1 designs. The optimized model has shown sufficient robustness even if the calibration sets were constructed from different sets of pure spectra of the components. Althougłrthc spectra of the components overlapped significantly, the drugs were determined accurately and precisely using the model. No interference from tablet excipients was observed.

PL

Opracowano metodę równoczesnego oznaczania wszystkich komponentów w wieloskładnikowym preparacie farmaceutycznym wykorzystując spektrometrie UV i model kalibra-cyjny oparty na sztucznych sieciach neuronowych. Proponowana metodą jest prosta alternatywą względem stosowania odrębnego modelu dla każdego związku- Metoda ta przedstawia nowy sposób kalibrowania, w którym wykorzystuje się zestaw obliczonych danych Opracowano metodę równoczesnego oznaczania wszystkich komponentów w wieloskładnikowym preparacie farmaceutycznym wykorzystując spektrometrie UV i model kalibra-cyjny oparty na sztucznych sieciach neuronowych. Proponowana metodą jest prosta alternatywą względem stosowania odrębnego modelu dla każdego związku- Metoda ta przedstawia nowy sposób kalibrowania, w którym wykorzystuje się zestaw obliczonych danych spektralnych otrzymanych z trzech widm każdego składnika. Widma atenololu i losartanu rejestrowano w zakresie 215-217 nm, co l nm, przy różnych stężeniach obu analitów, w liniowym zakresie kalibracji i wykorzystano do obliczenia składu miesznin kalibracyjnych. Do zbudowania i optymalizowania modelu kalibracji zastosowano sieci neuronowe trenowane algorytmem Levenberga-Marquardt'a za pomocą programu MATLAB Neu-ronal Network Toolbox. Dwa rodzaje takich modeli porównano z modelem regresji skianika głównego. Opracowany model kalibracji został szczegółowo zbadany dla wielu poziomów stężeń. Badania prowadzono przy użyciu widni 76 dwuskładnikowych mieszanin przygotowanych według modelu ortogonalnego. Zoptymalizowany model okazał się przydatny nawet wówczas gdy mieszaniny kalibracyjne były pr/ygotowane z różnych zestawów spek-tralnie czystych składników. Chociaż widma poszczególnych składników nakładały się w znacznym stopniu opracowany model pozwalał na dokładne i precyzyjne oznaczenie stężeń badanych leków. Nie zaobserwowano wpiywu substancji pomocniczych na jakość oznaczeń.

6

Kinetic methods of determination of atenolol in pure compound and in pharmaceutical formulations

Hiremath G. C., Mulla R. M., Nandibewoor S. T.

Chemia Analityczna

|

2005

|

Vol. 50, No. 2

449-455

EN

A sensitive and rapid kinetic method for the determination of atenolol in bulk drugs, pharmaceutical preparations, and urine and blood samples has been developed. The method is based on the oxidation of atenolol by permanganate in alkaline media. The results were compared with the values obtained with the official method. The differences between them were statistically insignificant, as t- and F-tests have revealed. No interference from the commonly used excipients was observed.

PL

Opracowano czułą i szybką kinetyczną metodę oznaczania atenololu w substancji i w formie farmaceutycznej oraz we krwi i w moczu. Metoda jest oparta na utlenianiu atenololu nadmanganianem w środowisku alkalicznym. Wyniki otrzymane za pomocą proponowanej metody porównano, używając statystycznych testów Studenta (t) i Fishera (F), z wynikami otrzymanymi oficjalnie przyjętą metodą. Obie metody dały zgodne rezultaty. Nie zaobserwowano interferencji ze strony popularnych farmaceutycznych substancji pomocniczych.

7

Determination of atenolol in pure substance and pharmaceutical preparations applying first order derivative spectrophotometry

Khan I. U., Yaqoob N., Ahmad M.

Chemia Analityczna

|

2005

|

Vol. 50, No. 5

951-955

EN

The improved first order derivative spectrophotometric method for the determination of atenolol in pure substance and pharmaceutical preparations has been proposed. The first order derivative spectrum of the coloured complex of atenolol with phenolsulfothaline exhibited maximum absorbance at 558.4 nm. The proposed approach allowed one to eliminate spectral interferences. The measured analytical signal changed linearly within the analyte concentration range: from 0.05 to 0.4 mg mL-1. Coefficient of variation was 0.854%. Detection limit was found to be 0.05 mg mL-1.

PL

Zaproponowano ulepszoną metodę spektrofotometrii pochodnej do oznaczania atenololu w substancji farmaceutycznej i w postaci leku. Pierwsza pochodna widma barwnego kompleksu atenololu 7. fenolosulfotalinąwykazaia maksimum absopcji przy 558,4 nm. Proponowana metoda pozwala na wyeliminowanie interferencji spektralnych. Sygnał analityczny miał liniowy przebieg w zakresie stężeń od 0,05 do 0,4 mg L-1. Współczynnik zmienności wynosił 0,854%. Granicę wykrywalności określono na 0,05 mg L-1.