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1 Polish Journal of Chemistry

1 2008

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Application of Enzyme Labeled Antibodies for Immunosensors Based on Electrochem ical Detection

Kazimierczak B., Pijanowska D., Torbicz W.

Polish Journal of Chemistry

2008

Vol. 82, nr 6

1255-1263

In this paper, immunosensors based on electrochemical (potentiometric and amperometric) detection are presented. We developed ISFET type potentiometric immunosensor with urease as a label and a screen-printed amperometric immunosensor with alkaline phosphatase, utilizing ascorbic acid phosphate as a substrate. For the free alkaline phosphatase in concentration range 35-3500 pM, the response of amperometric sensors was at the level from 10 to 80 miA. It was stated that the potentiometric immunosensors can be applied for measurements of the to tal gamma-globulin concentration or for detection of gamma the selected IgG in concentration range 1.37 mg/L-4 g/L.