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1 2012

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Application of HPLC, Gel electrophoresis and size exclusion chromatography for separation and characterization of storage proteins of soybean and peanut

Bhushan R., Dixit S.

Acta Chromatographica

2012

Vol. 24, no. 3

323--349

Certain practical aspects in the separation of large biomolecules have been discussed with the chosen examples of storage proteins from two important crops, viz, soybean and peanut. Importance of seeds and storage proteins along with classification and nomenclature of plant proteins is briefly mentioned. The present paper describes an updated knowledge and status on structural features of glycinin (major storage protein of soybean) and arachin (major storage protein of peanut) with respect to their molecular species, subunit structure, and their association-dissociation behavior by using techniques of high-performance liquid chromatography (HPLC), electrophoresis and size exclusion chromatography (SEC) which supplemented each other.