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Development of a highly sensitive HPLC method for the simultaneous determination of eight biogenic amines in aquatic products

Fu Qinyi, Zheng Hongwei, Han Xiangning, Cao Limin, Sui Jianxin

Acta Chromatographica

2021

Vol. 33, no. 4

378--386

A highly sensitive high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of eight biogenic amines in aquatic products. The biogenic amines in the sample were extracted with 5% trichloroacetic acid, derived with dansyl chloride (Dns-Cl) and quantified by a UV detector. The results showed that tryptamine (TRY), 2-phenethylamine (PHE), putrescine (PUT), cadaverine (CAD), histamine (HIS), tyramine (TPY), spermidine (SPD), and spermine (SPM) were effectively separated in 18 min in the range of 0.1–50 mg/kg with a good linear coefficient (r2 > 0.999). The detection limits (LODs) of the eight biogenic amines were 0.007–0.021 mg/kg while the limits of quantification (LOQs) were 0.024–0.069 mg/kg with the recoveries basically between 68 and 123%. The determination of eight biogenic amines in five commercial fermented aquatic products indicating that the developed method could be applied for the simultaneous detection of biogenic amines in multiple aquatic products.

Determination of formaldehyde in aquatic products by micellar electrokinetic capillary chromatography with 2,4-dinitrophenylhydrazine derivatization

Xu L. N, Gai F. Y, Mu G. F, Gao Y., Liu C. J., Luan F.

Acta Chromatographica

2012

Vol. 24, no. 4

519--528

Formaldehyde in aquatic products was determined by micellar electrokinetic capillary chromatography (MEKC) after derivatization with 2,4-dinitrophenylhydrazine. Separation was carried out at 25 °C and 25 kV, using a fused silica capillary (75 µ internal diameter; 50.5 cm effective length) and an ultraviolet detector set at 360 nm. The optimal background electrolyte was 20 mM sodium tetraborate and 20 mM sodium dodecyl sulfate at pH 9.0 with 3 s hydrodynamic injection at 30 mbar. Electrophoretic analysis took approximately 6.5 min. The correlation coefficient of the calibration curve was 0.999 over the concentration range 2.0–100.0 mg L-1 and the LOD and LOQ values were 0.57 and 1.89 µg mL-1, respectively. The recoveries were from 83.7% to 97.2% with steam distillation as the sample pretreatment method.