Wyniki wyszukiwania - BazTech

Ograniczanie wyników

1 Polish Journal of Chemistry

1 2002

Powiadomienia systemowe

Sesja wygasła!

Znaleziono wyników: 1

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: analogues of EETI-II

Sortuj według:

Ogranicz wyniki do:

Analogues of Ecballium elaterium Trypsin Inhibitor II (EETI-II) with L-Cysteine Residues Substituted by L-Penicillamine (Pen) and L-Homocysteine (Hcy) in Positions 19, 21 and 27

Frąszczak P., Kaźmierczak K., Stawikowski M., Jaśkiewicz A., Kupryszewski G., Rolka K.

Polish Journal of Chemistry

2002

Vol. 76 / nr 10

1441-1446

Two analogues of peptidic trypsin inhibitor isolated from seeds of Ecballium elaterium (EETI-II): [Pen19,21,27] EETI-II (1) and [Hcy19,21,27] EETI-II (2) were synthesized by the solid-phase method using the Fmoc/But procedure. Their inhibitory activity was determined by the calculation of association equilibrium constants (Ka) with bovine _-trypsin. In comparison with the parent compound, both analogues showed reduced trypsin inhibitory activity more than 7 and 18 times, respectively. We postulate that the observed differences may reflect the role of disulfide bridges in the interaction of inhibitors with trypsin or the introduced modifications change the conformational equilibrium of the analogues synthesized towards conformation(s) less favorable for the interaction with the enzyme.